Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor

2021 ◽  
Author(s):  
Kamil Wierzchowski ◽  
Maciej Pilarek
2010 ◽  
pp. 795-801 ◽  
Author(s):  
J. E. Schouwenberg ◽  
T. Van der Velden-de Groot ◽  
G. Blueml

2018 ◽  
Vol 46 (4) ◽  
pp. 616-626 ◽  
Author(s):  
Frederic Wolf ◽  
Diana M. Rojas González ◽  
Ulrich Steinseifer ◽  
Markus Obdenbusch ◽  
Werner Herfs ◽  
...  

2011 ◽  
Vol 63 (4) ◽  
pp. 345-350 ◽  
Author(s):  
Kui Xie ◽  
Xiao-Wei Zhang ◽  
Li Huang ◽  
Yi-Ting Wang ◽  
Yun Lei ◽  
...  

Author(s):  
Tobias Steinwedel ◽  
Katharina Dahlmann ◽  
Dörte Solle ◽  
Thomas Scheper ◽  
Kenneth F. Reardon ◽  
...  

2010 ◽  
pp. 371-373
Author(s):  
Nico M.G. Oosterhuis ◽  
Pieter van der Heiden

2010 ◽  
Vol 10 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Christoph Ries ◽  
Gernot John ◽  
Corinne John ◽  
Regine Eibl ◽  
Dieter Eibl

2020 ◽  
Vol 43 (11) ◽  
pp. 1973-1985
Author(s):  
Kamil Wierzchowski ◽  
Iwona Grabowska ◽  
Maciej Pilarek

Abstract Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (ReL) and the kLa coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVETM 25 system, on reached values of the apparent maximal specific growth rate (μmax) and the specific yield of biomass (Y*X/S) has been identified. The values of ReL (i.e., 510–10,208), as well as kLa coefficient (i.e., 2.83–13.55 h−1), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μmax = 0.038 h−1 and Y*X/S = 25.64 × 108 cells gglc−1 have been noted for cultures independently performed at wave-induced agitation characterized by ReL equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.


2006 ◽  
Vol 31 (3) ◽  
pp. 363-368 ◽  
Author(s):  
I. Slivac ◽  
V. Gaurina Srček ◽  
K. RadoŠević ◽  
I. Kmetič ◽  
Z. Kniewald

Sign in / Sign up

Export Citation Format

Share Document