scholarly journals Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media

2019 ◽  
pp. 275-291 ◽  
Author(s):  
Dani Do Hyang Lee ◽  
Alina Petris ◽  
Robert E. Hynds ◽  
Christopher O’Callaghan
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Culture Media ◽  
Liquid Interface ◽  
Epithelial Cell Differentiation ◽  
Ciliated Epithelial Cell ◽  
Air Liquid Interface

scholarly journals Role of foxj1 and estrogen receptor alpha in ciliated epithelial cell differentiation of the neonatal oviduct

2004 ◽  
Vol 32 (3) ◽  
pp. 615-625 ◽  
Author(s):  
A Okada ◽  
Y Ohta ◽  
SL Brody ◽  
H Watanabe ◽  
A Krust ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Cell Differentiation ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Critical Role ◽  
Proliferating Cells ◽  
Epithelial Cell Differentiation ◽  
Ciliated Epithelial Cell ◽  
Ciliated Epithelial Cells ◽  
Deficient Mice

Estrogen regulates proliferation and differentiation of epithelial cells in the mammalian oviduct, but pathways for cell-specific differentiation are not well understood. In the epithelial cells of the developing rat oviduct, we found estrogen receptor (ER) alpha is expressed at birth and persists in all cells through neonatal day (ND) 7 when ciliated cells appear. To determine a specific function of ER and foxj1, a transcription factor known to have fundamental roles in ciliogenesis in the lung, in differentiation of the ciliated epithelial cells, we treated newborn rats from ND 0 to 5 with estradiol-17beta (E2) with and without a selective ER antagonist. E2 enhanced the number of proliferating cells and accelerated the process of epithelial cell differentiation resulting in ciliogenesis by ND 5, and co-treatment with an ER antagonist inhibited these changes. Foxj1 was expressed only in the infundibulum and ampulla (INF/AMP). That expression preceded the appearance of cilia and was induced by E2. Cilia were absent in oviducts of foxj1-deficient mice, indicating that foxj1 plays a critical role in oviductal ciliogenesis. However, we found the presence of cilia in the ERalpha-deficient mouse oviduct. The widespread expression of ERalpha in oviductal epithelium, but restriction of cilia to the INF/AMP regions, and importantly, the presence of cilia in the ERalpha-deficient mice, suggested ER signaling is not essential for ciliated epithelial cell differentiation. These observations demonstrate that, although E2 stimulates the differentiation process of ciliated epithelial cells, foxj1 is directly required for epithelial cell ciliogenesis of the neonatal oviduct.

YAP Regulates NFI/KLF5 Transcriptional and Epigenetic Networks Directing Alveolar Epithelial Cell Differentiation

2020 ◽  
Author(s):  
Jason J. Gokey ◽  
John Snowball ◽  
Anusha Sridharan ◽  
Parvathi Sudha ◽  
Joseph A. Kitzmiller ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Alveolar Epithelial Cell ◽  
Alveolar Epithelial ◽  
Epithelial Cell Differentiation

Late gestational hyperprolactinemia accelerates mammary epithelial cell differentiation that leads to increased milk yield1

2013 ◽  
Vol 91 (3) ◽  
pp. 1102-1111 ◽  
Author(s):  
M. K. VanKlompenberg ◽  
R. Manjarin ◽  
J. F. Trott ◽  
H. F. McMicking ◽  
R. C. Hovey
Keyword(s):  
Cell Differentiation ◽  
Epithelial Cell ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Differentiation
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