Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

2017 ◽  
pp. 25-33 ◽  
Author(s):  
Gabriel L. Converse ◽  
Dandan Li ◽  
Eric E. Buse ◽  
Richard A. Hopkins ◽  
Omar S. Aljitawi
Keyword(s):  
Tissue Engineering ◽  
Wharton’S Jelly ◽  
Engineering Applications ◽  
Wharton's Jelly

scholarly journals Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172098 ◽  
Author(s):  
Sushma Jadalannagari ◽  
Gabriel Converse ◽  
Christopher McFall ◽  
Eric Buse ◽  
Michael Filla ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Engineering Applications ◽  
Wharton's Jelly

Wharton’s jelly-derived mesenchymal cells as a new source for the generation of microtissues for tissue engineering applications

2018 ◽  
Vol 150 (4) ◽  
pp. 379-393 ◽  
Author(s):  
D. Durand-Herrera ◽  
F. Campos ◽  
B. D. Jaimes-Parra ◽  
J. D. Sánchez-López ◽  
R. Fernández-Valadés ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mesenchymal Cells ◽  
Wharton’S Jelly ◽  
Engineering Applications ◽  
Wharton's Jelly

Vitrified Wharton’s jelly tissue as a biomaterial for multiple tissue engineering applications

2019 ◽  
Vol 36 (2) ◽  
pp. 139-142 ◽  
Author(s):  
Panagiotis Mallis ◽  
Dimitra Boulari ◽  
Panagiota Chachlaki ◽  
Catherine Stavropoulos Giokas ◽  
Efstathios Michalopoulos
Keyword(s):  
Tissue Engineering ◽  
Wharton’S Jelly ◽  
Engineering Applications ◽  
Wharton's Jelly

Spidroin Striped Micropattern Promotes Chondrogenic Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Anggraini Barlian ◽  
Dinda Hani’ah Arum Saputri ◽  
Adriel Hernando ◽  
Ekavianty Prajatelistia ◽  
Hutomo Tanoto
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Spider Silk ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Wharton's Jelly

Abstract Cartilage tissue engineering, particularly micropattern, can influence the biophysical properties of mesenchymal stem cells (MSCs) leading to chondrogenesis. In this research, human Wharton’s jelly MSCs (hWJ-MSCs) were grown on a striped micropattern containing spider silk protein (spidroin) from Argiope appensa. This research aims to direct hWJ-MSCs chondrogenesis using micropattern made of spidroin bioink as opposed to fibronectin that often used as the gold standard. Cells were cultured on striped micropattern of 500 µm and 1000 µm width sizes without chondrogenic differentiation medium for 21 days. The immunocytochemistry result showed that spidroin contains RGD sequences and facilitates cell adhesion via integrin β1. Chondrogenesis was observed through the expression of glycosaminoglycan, type II collagen, and SOX9. The result on glycosaminoglycan content proved that 1000 µm was the optimal width to support chondrogenesis. Spidroin micropattern induced significantly higher expression of SOX9 mRNA on day-21 and SOX9 protein was located inside the nucleus starting from day-7. COL2A1 mRNA of spidroin micropattern groups was downregulated on day-21 and collagen type II protein was detected starting from day-14. These results showed that spidroin micropattern enhances chondrogenic markers while maintains long-term upregulation of SOX9, and therefore has the potential as a new method for cartilage tissue engineering.

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