scholarly journals Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells

2017 ◽  
pp. 283-290 ◽  
Author(s):  
Yoni Baert ◽  
Charlotte Rombaut ◽  
Ellen Goossens
Keyword(s):  
Testicular Cells

Restraint stress of male mice triggers apoptosis in spermatozoa and spermatogenic cells via activating the TNF-α system

Zygote ◽  
2020 ◽  
Vol 28 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Jie Zhang ◽  
De-Ling Kong ◽  
Bin Xiao ◽  
Hong-Jie Yuan ◽  
Qiao-Qiao Kong ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Restraint Stress ◽  
Semen Quality ◽  
Sperm Quality ◽  
Fertilization Rate ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Testicular Cells ◽  
Tnf Α

SummaryStudies have indicated that psychological stress impairs human fertility and that various stressors can induce apoptosis of testicular cells. However, the mechanisms by which psychological stress on males reduces semen quality and stressors induce apoptosis in testicular cells are largely unclear. Using a psychological (restraint) stress mouse model, we tested whether male psychological stress triggers apoptosis of spermatozoa and spermatogenic cells through activating tumour necrosis factor (TNF)-α signalling. Wild-type or TNF-α−/− male mice were restrained for 48 h before examination for apoptosis and expression of TNF-α and TNF receptor 1 (TNFR1) in spermatozoa, epididymis, seminiferous tubules and spermatogenic cells. The results showed that male restraint significantly decreased fertilization rate and mitochondrial membrane potential, while increasing levels of malondialdehyde, active caspase-3, TNF-α and TNFR1 in spermatozoa. Male restraint also increased apoptosis and expression of TNF-α and TNFR1 in caudae epididymides, seminiferous tubules and spermatogenic cells. Sperm quality was also significantly impaired when spermatozoa were recovered 35 days after male restraint. The restraint-induced damage to spermatozoa, epididymis and seminiferous tubules was significantly ameliorated in TNF-α−/− mice. Furthermore, incubation with soluble TNF-α significantly reduced sperm motility and fertilizing potential. Taken together, the results demonstrated that male psychological stress induces apoptosis in spermatozoa and spermatogenic cells through activating the TNF-α system and that the stress-induced apoptosis in spermatogenic cells can be translated into impaired quality in future spermatozoa.

scholarly journals Fighting Bisphenol A-Induced Male Infertility: The Power of Antioxidants

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 289
Author(s):  
Joana Santiago ◽  
Joana V. Silva ◽  
Manuel A. S. Santos ◽  
Margarida Fardilha
Keyword(s):  
Male Infertility ◽  
Bisphenol A ◽  
Sperm Concentration ◽  
In Vivo Studies ◽  
Sperm Dna Damage ◽  
Testicular Cells ◽  
Low Concentrations ◽  
Sperm Dna ◽  
Male Reproductive Health

Bisphenol A (BPA), a well-known endocrine disruptor present in epoxy resins and polycarbonate plastics, negatively disturbs the male reproductive system affecting male fertility. In vivo studies showed that BPA exposure has deleterious effects on spermatogenesis by disturbing the hypothalamic–pituitary–gonadal axis and inducing oxidative stress in testis. This compound seems to disrupt hormone signalling even at low concentrations, modifying the levels of inhibin B, oestradiol, and testosterone. The adverse effects on seminal parameters are mainly supported by studies based on urinary BPA concentration, showing a negative association between BPA levels and sperm concentration, motility, and sperm DNA damage. Recent studies explored potential approaches to treat or prevent BPA-induced testicular toxicity and male infertility. Since the effect of BPA on testicular cells and spermatozoa is associated with an increased production of reactive oxygen species, most of the pharmacological approaches are based on the use of natural or synthetic antioxidants. In this review, we briefly describe the effects of BPA on male reproductive health and discuss the use of antioxidants to prevent or revert the BPA-induced toxicity and infertility in men.

Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Zygote ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 432-435
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Gabriela Esteves Duarte ◽  
José Antonio Visintin ◽  
Mayra Elena Ortiz D’Ávila Assumpção
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Heat Stress ◽  
Glutathione Peroxidase ◽  
Cell Types ◽  
Treated Group ◽  
Oxidative Balance ◽  
Testicular Cells

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

Histone acetylation plays an important role in MC-LR-induced apoptosis and cycle disorder in SD rat testicular cells

Chemosphere ◽  
2020 ◽  
Vol 241 ◽  
pp. 125073 ◽  
Author(s):  
Yueqin Wang ◽  
Haohao Liu ◽  
Xiaohui Liu ◽  
Xiaofeng Zhang ◽  
Jinxia Wu ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Testicular Cells ◽  
Sd Rat ◽  
Induced Apoptosis

Localization of mouse type 2 Alu sequence in schistosomes

Parasitology ◽  
1999 ◽  
Vol 119 (3) ◽  
pp. 315-321 ◽  
Author(s):  
A. IMASE ◽  
T. KUMAGAI ◽  
H. OHMAE ◽  
Y. IRIE ◽  
Y. IWAMURA
Keyword(s):  
Dna Sequences ◽  
Mouse Genome ◽  
Testicular Cells ◽  
Alu Sequence ◽  
Vitelline Cells ◽  
Highly Repetitive Dna ◽  
Hybridization Band ◽  
Polymerase Chain

Localization of the type 2 Alu sequence (B2), a highly repetitive DNA sequence in the mouse genome, was examined by in situ polymerase chain reaction (in situ PCR) in schistosomes. The signals to the B2 sequence were detected in the cytoplasm of the tegumental membrane and in the nuclei of the mesenchymal, testicular, ovarian and vitelline cells of 8- week Schistosoma japonicum. In contrast, it was difficult to detect any signals of this sequence in 8-week S. mansoni, whereas in 24-week male S. mansoni the signals were observed in the cytoplasm of the tegumental tubercles and in the nuclei of the mesenchymal and testicular cells. On the other hand, in 24-week female S. mansoni the signals were found in the nuclei of the mesenchymal, ovarian and vitelline cells but not found in the tegument. On the contrary, no hybridization band of the B2 sequence was detected in the amplified DNA of 3-week schistosomula of either species. These observations proved that the host DNA sequences existed in restricted schistosome cells and were accumulated in the schistosome body during their development.

scholarly journals Germ cell-induced immune suppression in mice. Effect of inoculation of syngeneic spermatozoa on cell-mediated immune responses.

1982 ◽  
Vol 155 (6) ◽  
pp. 1719-1729 ◽  
Author(s):  
U Hurtenbach ◽  
G M Shearer
Keyword(s):  
Germ Cells ◽  
Immune Suppression ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Seminiferous Tubules ◽  
Immune Functions ◽  
Cytotoxic Lymphocyte ◽  
Testicular Cells ◽  
Killer Cell Activity

Spleen cells from mice injected intravenously with syngeneic male germ cells exhibited reduced immune functions as determined by natural killer cell activity, mixed lymphocyte reactivity and cytotoxic lymphocyte (CTL) function. The decrease in CTL responses to trinitrophenyl-modified self (TNP-self) was detected as early as 4 d after sperm injection and was observed to H-2 alloantigens 3 wk after injection. Radiosensitive suppressor T cells were found to suppress the CTL response to TNP-self. Suppression lasted for a period of at least 7 wk after a single inoculation of the germ cells. Some variability in immune suppression capability was observed using different preparations of germ cells which are not yet completely understood. Sperm were more effective in inducing suppression than testicular cells derived from the seminiferous tubules. Furthermore, sperm from older animals were more effective than those from younger mice. These findings are discussed with respect to possible regulatory influences of germ cells on the immune system when the blood-testes barrier is broken.

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