scholarly journals 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering

2017 ◽  
pp. 173-180 ◽  
Author(s):  
Shrestha Priyadarsini ◽  
Sarah E. Nicholas ◽  
Dimitrios Karamichos
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue

Use of Polycaprolactone in Corneal Tissue Engineering: A Review

2021 ◽  
pp. 102402
Author(s):  
Amin Orash Mahmoud Salehi ◽  
Saeed Heidari Keshel ◽  
Farshid Sefat ◽  
Lobat Tayebi
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue

scholarly journals Significance of Crosslinking Approaches in the Development of Next Generation Hydrogels for Corneal Tissue Engineering

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 319
Author(s):  
Promita Bhattacharjee ◽  
Mark Ahearne
Keyword(s):  
Tissue Engineering ◽  
Chemical Structure ◽  
Biochemical Properties ◽  
Corneal Tissue ◽  
Future Prospects ◽  
Chemical Additives ◽  
Injectable Hydrogels ◽  
Corneal Regeneration ◽  
Key Factor ◽  
Fuchs Endothelial Dystrophy

Medical conditions such as trachoma, keratoconus and Fuchs endothelial dystrophy can damage the cornea, leading to visual deterioration and blindness and necessitating a cornea transplant. Due to the shortage of donor corneas, hydrogels have been investigated as potential corneal replacements. A key factor that influences the physical and biochemical properties of these hydrogels is how they are crosslinked. In this paper, an overview is provided of different crosslinking techniques and crosslinking chemical additives that have been applied to hydrogels for the purposes of corneal tissue engineering, drug delivery or corneal repair. Factors that influence the success of a crosslinker are considered that include material composition, dosage, fabrication method, immunogenicity and toxicity. Different crosslinking techniques that have been used to develop injectable hydrogels for corneal regeneration are summarized. The limitations and future prospects of crosslinking strategies for use in corneal tissue engineering are discussed. It is demonstrated that the choice of crosslinking technique has a significant influence on the biocompatibility, mechanical properties and chemical structure of hydrogels that may be suitable for corneal tissue engineering and regenerative applications.

Characterization of a Novel Collagen Scaffold for Corneal Tissue Engineering

2016 ◽  
Vol 22 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Jie Zhang ◽  
Aran M.G. Sisley ◽  
Alexander J. Anderson ◽  
Andrew J. Taberner ◽  
Charles N.J. McGhee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Collagen Scaffold ◽  
Corneal Tissue

scholarly journals High-Resolution Bioprinting of Recombinant Human Collagen Type III

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2973
Author(s):  
Rory Gibney ◽  
Jennifer Patterson ◽  
Eleonora Ferraris
Keyword(s):  
Tissue Engineering ◽  
High Resolution ◽  
Collagen Type ◽  
Thin Layers ◽  
Corneal Tissue ◽  
Collagen Type Iii ◽  
Type Iii ◽  
Pure Collagen ◽  
Human Collagen ◽  
Visible Wavelengths

The development of commercial collagen inks for extrusion-based bioprinting has increased the amount of research on pure collagen bioprinting, i.e., collagen inks not mixed with gelatin, alginate, or other more common biomaterial inks. New printing techniques have also improved the resolution achievable with pure collagen bioprinting. However, the resultant collagen constructs still appear too weak to replicate dense collagenous tissues, such as the cornea. This work aims to demonstrate the first reported case of bioprinted recombinant collagen films with suitable optical and mechanical properties for corneal tissue engineering. The printing technology used, aerosol jet® printing (AJP), is a high-resolution printing method normally used to deposit conductive inks for electronic printing. In this work, AJP was employed to deposit recombinant human collagen type III (RHCIII) in overlapping continuous lines of 60 µm to form thin layers. Layers were repeated up to 764 times to result in a construct that was considered a few hundred microns thick when swollen. Samples were subsequently neutralised and crosslinked using EDC:NHS crosslinking. Nanoindentation and absorbance measurements were conducted, and the results show that the AJP-deposited RHCIII samples possess suitable mechanical and optical properties for corneal tissue engineering: an average effective elastic modulus of 506 ± 173 kPa and transparency ≥87% at all visible wavelengths. Circular dichroism showed that there was some loss of helicity of the collagen due to aerosolisation. SDS-PAGE and pepsin digestion were used to show that while some collagen is degraded due to aerosolisation, it remains an inaccessible substrate for pepsin cleavage.

scholarly journals Biomimetic vs. Direct Approach to Deposit Hydroxyapatite on the Surface of Low Melting Point Polymers for Tissue Engineering

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2162
Author(s):  
Andri K. Riau ◽  
Subbu S. Venkatraman ◽  
Jodhbir S. Mehta
Keyword(s):  
Tissue Engineering ◽  
Surrounding Tissue ◽  
Processing Temperature ◽  
Corneal Tissue ◽  
Cellular Functions ◽  
Advantages And Disadvantages ◽  
Phase Coating ◽  
Polymeric Substrates ◽  
Immobilization Techniques ◽  
High Processing

Polymers are widely used in many applications in the field of biomedical engineering. Among eclectic selections of polymers, those with low melting temperature (Tm < 200 °C), such as poly(methyl methacrylate), poly(lactic-co-glycolic acid), or polyethylene, are often used in bone, dental, maxillofacial, and corneal tissue engineering as substrates or scaffolds. These polymers, however, are bioinert, have a lack of reactive surface functional groups, and have poor wettability, affecting their ability to promote cellular functions and biointegration with the surrounding tissue. Improving the biointegration can be achieved by depositing hydroxyapatite (HAp) on the polymeric substrates. Conventional thermal spray and vapor phase coating, including the Food and Drug Administration (FDA)-approved plasma spray technique, is not suitable for application on the low Tm polymers due to the high processing temperature, reaching more than 1000 °C. Two non-thermal HAp coating approaches have been described in the literature, namely, the biomimetic deposition and direct nanoparticle immobilization techniques. In the current review, we elaborate on the unique features of each technique, followed by discussing the advantages and disadvantages of each technique to help readers decide on which method is more suitable for their intended applications. Finally, the future perspectives of the non-thermal HAp coating are given in the conclusion.

Nanofibrous scaffolds for corneal tissue engineering

2013 ◽  
Vol 91 ◽  
pp. 0-0
Author(s):  
T FUCHSLUGER ◽  
T BAHNERS ◽  
M CZUGALA ◽  
J GUTMANN ◽  
S SALEHI
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue ◽  
Nanofibrous Scaffolds

scholarly journals Decellularized Human Stromal Lenticules Combine with Corneal Epithelial-Like Cells: A New Resource for Corneal Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Shuai Qin ◽  
Shuai Zheng ◽  
Bing Qi ◽  
Rui Guo ◽  
Guanghui Hou
Keyword(s):  
Tissue Engineering ◽  
Induction Medium ◽  
Nuclear Materials ◽  
Corneal Tissue ◽  
Corneal Epithelial ◽  
Human Ipscs ◽  
Immunological Rejection ◽  
Lenticule Extraction ◽  
Induced Pluripotent

The lack of donor corneal tissue or the immunological rejection remains a challenge for individuals with limbal stem cell deficiency (LSCD) who are treated with keratoplasty. Numerous lenticules which were extracted by small incision lenticule extraction (SMILE) appear to be useful materials for keratoplasty. In order to reduce the incidence of allograft rejection, lenticules would be decellularized. Lenticules which were treated with liquid nitrogen and nucleases had no cellular and nuclear materials remained. Human induced pluripotent stem cells (iPSCs) can be generated from the patient who requires keratoplasty, offering an autologous alternative and eliminating the risk of graft rejection. We found that BMP-4, RA, N-2 supplement, hEGF, B27, decellularized human stromal lenticules, conditioned medium, or induction medium promoted the differentiation of human iPSCs with high purity. The results showed that human iPSCs cultured for 4 days in differentiation medium A, 14 days in condition medium, and 1 week in induction medium on decellularized human stromal lenticules developed markedly higher expression of the markers P63, CK3, and CK12 than did those in the other methods. The level of gene expression of the epithelial and pluripotency markers and analysis by scanning electron microscopy and immunohistochemistry also showed successful differentiation. After inducing differentiation in vitro, corneal epithelial-like cells were induced. In the study, we investigated the possibility of a new resource for corneal tissue engineering.

Synthetic neoglycopolymer-recombinant human collagen hybrids as biomimetic crosslinking agents in corneal tissue engineering

Biomaterials ◽  
2009 ◽  
Vol 30 (29) ◽  
pp. 5403-5408 ◽  
Author(s):  
Kimberley Merrett ◽  
Wenguang Liu ◽  
Debbie Mitra ◽  
Kenneth D. Camm ◽  
Christopher R. McLaughlin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Corneal Tissue ◽  
Human Collagen ◽  
Crosslinking Agents
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