MALDI Imaging Combined with Laser Microdissection-Based Microproteomics for Protein Identification: Application to Intratumor Heterogeneity Studies

2017 ◽  
pp. 297-312 ◽  
Author(s):  
Rémi Longuespée ◽  
Deborah Alberts ◽  
Dominique Baiwir ◽  
Gabriel Mazzucchelli ◽  
Nicolas Smargiasso ◽  
...  
Keyword(s):  
Protein Identification ◽  
Laser Microdissection ◽  
Intratumor Heterogeneity ◽  
Maldi Imaging

Abstract LB-187: MALDI imaging-guided microproteomics workflow for intratumor heterogeneity studies

2016 ◽  
Author(s):  
Deborah Alberts ◽  
Rémi Longuespée ◽  
Charles Pottier ◽  
Nicolas Smargiassio ◽  
Gabriel Mazzucchelli ◽  
...  
Keyword(s):  
Intratumor Heterogeneity ◽  
Maldi Imaging

scholarly journals Improved Reproducibility of Protein Detection in Neuromelanin-containing Human Substantia Nigra Tissue enriched by Laser Microdissection

2020 ◽  
Author(s):  
Steffen Kösters ◽  
Mandy Rettel ◽  
Britta Eggers ◽  
Peter Riederer ◽  
Manfred Gerlach ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Brain Tissue ◽  
Protein Identification ◽  
Laser Microdissection ◽  
Protein Detection ◽  
Protein Quantification ◽  
Human Brain Tissue ◽  
Limited Sample ◽  
Tissue Samples ◽  
Proteomics Research

Abstract Background: Processing of post-mortem human brain tissue samples like Neuromelanin-containing substantia nigra is a challenging task in proteomics research in the context of neurodegenerative diseases such as Parkinson’s disease, due to the availability of only limited sample amounts. Therefore, a highly efficient lysis and digestion approach is needed to guarantee high reproducibility and a good quantification performance. We conducted a systematic study in order to compare qualitatively and quantitatively six different sample preparation protocols for substantia nigra brain tissue collected by laser microdissection.Results: LC-MS/MS analysis revealed that the combination of formic acid and in-solution digestion with trypsin leads to an enhanced peptide and protein identification as well as a robust and reliable protein quantification with a very good reproducibility.Conclusion: We recommend this protocol for future studies of human substantia nigra tissue as well as NM granules in the context of neurodegenerative disorders like Parkinson syndrome.

scholarly journals Improved Reproducibility of Protein Detection in Neuromelanin-containing Human Substantia Nigra Tissue enriched by Laser Microdissection

2020 ◽  
Author(s):  
Steffen Kösters ◽  
Mandy Rettel ◽  
Britta Eggers ◽  
Peter Riederer ◽  
Manfred Gerlach ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Brain Tissue ◽  
Protein Identification ◽  
Laser Microdissection ◽  
Protein Detection ◽  
Protein Quantification ◽  
Human Brain Tissue ◽  
Limited Sample ◽  
Tissue Samples ◽  
Proteomics Research

Abstract Background: Processing of post-mortem human brain tissue samples like Neuromelanin-containing substantia nigra is a challenging task in proteomics research in the context of neurodegenerative diseases such as Parkinson’s disease, due to the availability of only limited sample amounts. Therefore, a highly efficient lysis and digestion approach is needed to guarantee high reproducibility and a good quantification performance. We conducted a systematic study in order to compare qualitatively and quantitatively six different sample preparation protocols for substantia nigra brain tissue collected by laser microdissection.Results: LC-MS/MS analysis revealed that the combination of formic acid and in-solution digestion with trypsin leads to an enhanced peptide and protein identification as well as a robust and reliable protein quantification with a very good reproducibility.Conclusion: We recommend this protocol for future studies of human substantia nigra tissue as well as NM granules in the context of neurodegenerative disorders like Parkinson syndrome.

MALDI-imaging: a tool to study lipid distribution in liver tissue during regeneration

2010 ◽  
Vol 48 (01) ◽  
Author(s):  
S Zellmer ◽  
A Veloso ◽  
E Astigarraga ◽  
JA Fernández ◽  
R Gebhardt
Keyword(s):  
Liver Tissue ◽  
Maldi Imaging ◽  
Lipid Distribution

Deep proteomic characterization of fiber types of murine muscles by laser microdissection and mass spectrometry

2019 ◽  
Author(s):  
B Eggers ◽  
K Schork ◽  
R Schröder ◽  
CS Clemen ◽  
C Berwanger ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Microdissection ◽  
Fiber Types
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