Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter

2016 ◽  
pp. 57-89 ◽  
Author(s):  
Leonid V. Bystrykh ◽  
Mirjam E. Belderbos
Keyword(s):  
Clonal Analysis ◽  
Cellular Barcoding

Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding

2013 ◽  
Vol 41 (8) ◽  
pp. S38
Author(s):  
Evgenia Verovskaya ◽  
Mathilde Broekhuis ◽  
Erik Zwart ◽  
Martha Ritsema ◽  
Ronald van Os ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Clonal Analysis ◽  
Hematopoietic Stem ◽  
Cellular Barcoding

Cellular barcoding tool for clonal analysis in the hematopoietic system

Blood ◽  
2010 ◽  
Vol 115 (13) ◽  
pp. 2610-2618 ◽  
Author(s):  
Alice Gerrits ◽  
Brad Dykstra ◽  
Olga J. Kalmykowa ◽  
Karin Klauke ◽  
Evgenia Verovskaya ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Integration Site ◽  
Retroviral Vectors ◽  
Clonal Analysis ◽  
Great Promise ◽  
Hematopoietic Stem ◽  
Site Analysis ◽  
Integration Site Analysis ◽  
Cellular Barcoding

Abstract Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro cell expansion, gene therapy, and cancer progression and treatment. A common approach to measure clonality of retrovirally transduced cells is to perform integration site analysis using Southern blotting or polymerase chain reaction–based methods. Although these methods are useful in principle, they generally provide a low-resolution, biased, and incomplete assessment of clonality. To overcome those limitations, we labeled retroviral vectors with random sequence tags or “barcodes.” On integration, each vector introduces a unique, identifiable, and heritable mark into the host cell genome, allowing the clonal progeny of each cell to be tracked over time. By coupling the barcoding method to a sequencing-based detection system, we could identify major and minor clones in 2 distinct cell culture systems in vitro and in a long-term transplantation setting. In addition, we demonstrate how clonal analysis can be complemented with transgene expression and integration site analysis. This cellular barcoding tool permits a simple, sensitive assessment of clonality and holds great promise for future gene therapy protocols in humans, and any other applications when clonal tracking is important.

Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding

Blood ◽  
2013 ◽  
Vol 122 (4) ◽  
pp. 523-532 ◽  
Author(s):  
Evgenia Verovskaya ◽  
Mathilde J. C. Broekhuis ◽  
Erik Zwart ◽  
Martha Ritsema ◽  
Ronald van Os ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Clonal Analysis ◽  
Hematopoietic Stem ◽  
Key Points ◽  
Cellular Barcoding

Key Points Quantitative clonal analysis demonstrates directional changes in contributions of stem cells to blood. The pool of aged hematopoietic stem cells is comprised of many, but small clones, while young stem cells are less numerous, but more potent.

Clonal analysis of multifocal low-grade MALT lymphomas

2001 ◽  
Vol 120 (5) ◽  
pp. A298-A298
Author(s):  
M IWANO ◽  
Y MATSUSHIMA ◽  
K OKAZAKI ◽  
T CHIBA
Keyword(s):  
Clonal Analysis ◽  
Low Grade

Cellular barcoding as a tool to investigate the clonal complexity and evolution of ALL

2015 ◽  
Vol 227 (03) ◽  
Author(s):  
AK Elder ◽  
O Heidenreich ◽  
HJ Vormoor
Keyword(s):  
Cellular Barcoding

Clonal Analysis of Sacrococcygeal “Teratomas”

1996 ◽  
Vol 16 (6) ◽  
pp. 865-875
Author(s):  
K. L. Sinnock ◽  
A. R. Perez-Atayde ◽  
K. A. Boynton ◽  
G. L. Mutter
Keyword(s):  
Clonal Analysis
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