Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons

2015 ◽  
pp. 111-131 ◽  
Author(s):  
Shin-Il Kim ◽  
Fabian Oceguera-Yanez ◽  
Chiho Sakurai ◽  
Masato Nakagawa ◽  
Shinya Yamanaka ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Ips Cells ◽  
Human Ips Cells

Hypothalamic contribution to pituitary functions is recapitulated in vitro using 3D-cultured human iPS cells

2020 ◽  
Author(s):  
Kasai T ◽  
Suga H ◽  
Sakakibara   ◽  
Ozone C ◽  
Matsumoto R ◽  
...  
Keyword(s):  
Ips Cells ◽  
Human Ips Cells

scholarly journals Genetically Matched Human iPS Cells Reveal that Propensity for Cartilage and Bone Differentiation Differs with Clones, not Cell Type of Origin

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53771 ◽  
Author(s):  
Akira Nasu ◽  
Makoto Ikeya ◽  
Takuya Yamamoto ◽  
Akira Watanabe ◽  
Yonghui Jin ◽  
...  
Keyword(s):  
Ips Cells ◽  
Cell Type ◽  
Bone Differentiation ◽  
Human Ips Cells

scholarly journals Protein-based human iPS cells efficiently generate functional dopamine neurons and can treat a rat model of Parkinson disease

2011 ◽  
Vol 121 (6) ◽  
pp. 2326-2335 ◽  
Author(s):  
Yong-Hee Rhee ◽  
Ji-Yun Ko ◽  
Mi-Yoon Chang ◽  
Sang-Hoon Yi ◽  
Dohoon Kim ◽  
...  
Keyword(s):  
Parkinson Disease ◽  
Rat Model ◽  
Ips Cells ◽  
Dopamine Neurons ◽  
Generate Functional ◽  
Human Ips Cells

scholarly journals Bone Morphogenetic Protein 4 (BMP4) Enhances the Differentiation of Human Induced Pluripotent Stem Cells into Limbal Progenitor Cells

2021 ◽  
Vol 43 (3) ◽  
pp. 2124-2134
Author(s):  
Hyun Soo Lee ◽  
Jeewon Mok ◽  
Choun-Ki Joo
Keyword(s):  
Progenitor Cells ◽  
Dermal Fibroblast ◽  
Ips Cells ◽  
Human Dermal Fibroblast ◽  
Corneal Epithelial ◽  
Specific Medium ◽  
Morphogenetic Protein ◽  
Human Ips Cells ◽  
Limbal Stem Cell ◽  
Induced Pluripotent

Corneal epithelium maintains visual acuity and is regenerated by the proliferation and differentiation of limbal progenitor cells. Transplantation of human limbal progenitor cells could restore the integrity and functionality of the corneal surface in patients with limbal stem cell deficiency. However, multiple protocols are employed to differentiate human induced pluripotent stem (iPS) cells into corneal epithelium or limbal progenitor cells. The aim of this study was to optimize a protocol that uses bone morphogenetic protein 4 (BMP4) and limbal cell-specific medium. Human dermal fibroblast-derived iPS cells were differentiated into limbal progenitor cells using limbal cell-specific (PI) medium and varying doses (1, 10, and 50 ng/mL) and durations (1, 3, and 10 days) of BMP4 treatment. Differentiated human iPS cells were analyzed by real-time polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemical studies at 2 or 4 weeks after BMP4 treatment. Culturing human dermal fibroblast-derived iPS cells in limbal cell-specific medium and BMP4 gave rise to limbal progenitor and corneal epithelial-like cells. The optimal protocol of 10 ng/mL and three days of BMP4 treatment elicited significantly higher limbal progenitor marker (ABCG2, ∆Np63α) expression and less corneal epithelial cell marker (CK3, CK12) expression than the other combinations of BMP4 dose and duration. In conclusion, this study identified a successful reprogramming strategy to induce limbal progenitor cells from human iPS cells using limbal cell-specific medium and BMP4. Additionally, our experiments indicate that the optimal BMP4 dose and duration favor limbal progenitor cell differentiation over corneal epithelial cells and maintain the phenotype of limbal stem cells. These findings contribute to the development of therapies for limbal stem cell deficiency disorders.

scholarly journals Use of a Sendai virus-based vector for effcient transduction of pinniped fbroblasts

2019 ◽  
Vol 22 (8) ◽  
pp. 1020-1025 ◽  
Author(s):  
V. R. Beklemisheva ◽  
A. G. Menzorov
Keyword(s):  
Transgene Expression ◽  
Viral Vectors ◽  
Lentiviral Vector ◽  
Sendai Virus ◽  
Homo Sapiens ◽  
Fluorescent Proteins ◽  
American Mink ◽  
Ips Cells ◽  
Canis Lupus Familiaris ◽  
Induced Pluripotent

Generation of induced pluripotent stem (iPS) cells expanded possibilities of pluripotency and early development studies. Generation of order Carnivora iPS cells from dog (Canis lupus familiaris), snow leopard (Panthera uncia), and American mink (Neovison vison) was previously reported. The aim of the current study was to examine conditions of pinniped fbroblast reprogramming. Pinnipeds are representatives of the suborder Caniformia sharing conservative genomes. There are several ways to deliver reprogramming transcription factors: RNA, proteins, plasmids, viral vectors etc. The most effective delivery systems for mouse and human cells are based on viral vectors. We compared a lentiviral vector which integrates into the genome and a Sendai virus­based vector, CytoTune EmGFP Sendai Fluorescence Reporter. The main advantage of Sendai virus­based vectors is that they do not integrate into the genome. We performed delivery of genetic constructions carrying fluorescent proteins to fbroblasts of seven Pinnipeds: northern fur seal (Callorhinus ursinus), Steller sea lion (Eumetopias jubatus), walrus (Odobenus rosmarus), bearded seal (Erignathus barbatus), Baikal seal (Pusa sibirica), ringed seal (Phoca hispida), and spotted seal (Phoca largha). We also transduced American mink (N. vison), human (Homo sapiens), and mouse (Mus musculus) fbroblasts as a control. We showed that the Sendai virus­based transduction system provides transgene expression one­two orders of magnitude higher than the lentiviral system at a comparable multiplicity of infection. Also, transgene expression after Sendai virus­based transduction is quite stable and changes only slightly at day four compared to day two. These data allow us to suggest that Sendai virus­based vectors are preferable for generation of Pinniped iPS cells.

Generation and application of human iPS cells

2009 ◽  
Vol 54 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Chun Cui ◽  
LingJun Rao ◽  
LinZhao Cheng ◽  
Lei Xiao
Keyword(s):  
Ips Cells ◽  
Human Ips Cells
Sign in / Sign up

Export Citation Format

Share Document