Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells

2015 ◽  
pp. 295-310 ◽  
Author(s):  
Ayaka Yanagida ◽  
Hiromitsu Nakauchi ◽  
Akihide Kamiya
Keyword(s):  
Progenitor Cells ◽  
Ips Cells ◽  
Hepatic Progenitor Cells ◽  
In Vitro Expansion ◽  
Human Ips Cells

Hypothalamic contribution to pituitary functions is recapitulated in vitro using 3D-cultured human iPS cells

2020 ◽  
Author(s):  
Kasai T ◽  
Suga H ◽  
Sakakibara   ◽  
Ozone C ◽  
Matsumoto R ◽  
...  
Keyword(s):  
Ips Cells ◽  
Human Ips Cells

scholarly journals Bone Morphogenetic Protein 4 (BMP4) Enhances the Differentiation of Human Induced Pluripotent Stem Cells into Limbal Progenitor Cells

2021 ◽  
Vol 43 (3) ◽  
pp. 2124-2134
Author(s):  
Hyun Soo Lee ◽  
Jeewon Mok ◽  
Choun-Ki Joo
Keyword(s):  
Progenitor Cells ◽  
Dermal Fibroblast ◽  
Ips Cells ◽  
Human Dermal Fibroblast ◽  
Corneal Epithelial ◽  
Specific Medium ◽  
Morphogenetic Protein ◽  
Human Ips Cells ◽  
Limbal Stem Cell ◽  
Induced Pluripotent

Corneal epithelium maintains visual acuity and is regenerated by the proliferation and differentiation of limbal progenitor cells. Transplantation of human limbal progenitor cells could restore the integrity and functionality of the corneal surface in patients with limbal stem cell deficiency. However, multiple protocols are employed to differentiate human induced pluripotent stem (iPS) cells into corneal epithelium or limbal progenitor cells. The aim of this study was to optimize a protocol that uses bone morphogenetic protein 4 (BMP4) and limbal cell-specific medium. Human dermal fibroblast-derived iPS cells were differentiated into limbal progenitor cells using limbal cell-specific (PI) medium and varying doses (1, 10, and 50 ng/mL) and durations (1, 3, and 10 days) of BMP4 treatment. Differentiated human iPS cells were analyzed by real-time polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemical studies at 2 or 4 weeks after BMP4 treatment. Culturing human dermal fibroblast-derived iPS cells in limbal cell-specific medium and BMP4 gave rise to limbal progenitor and corneal epithelial-like cells. The optimal protocol of 10 ng/mL and three days of BMP4 treatment elicited significantly higher limbal progenitor marker (ABCG2, ∆Np63α) expression and less corneal epithelial cell marker (CK3, CK12) expression than the other combinations of BMP4 dose and duration. In conclusion, this study identified a successful reprogramming strategy to induce limbal progenitor cells from human iPS cells using limbal cell-specific medium and BMP4. Additionally, our experiments indicate that the optimal BMP4 dose and duration favor limbal progenitor cell differentiation over corneal epithelial cells and maintain the phenotype of limbal stem cells. These findings contribute to the development of therapies for limbal stem cell deficiency disorders.

In Vitro Expansion of a Multipotent Population of Human Neural Progenitor Cells

1999 ◽  
Vol 158 (2) ◽  
pp. 265-278 ◽  
Author(s):  
Melissa K. Carpenter ◽  
Xia Cui ◽  
Zhong-yi Hu ◽  
Jennifer Jackson ◽  
Sandy Sherman ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
In Vitro Expansion ◽  
Human Neural Progenitor Cells

In Vitro Differentiation of Human iPS Cells into Neural like Cells on a Biomimetic Polyurea

2016 ◽  
Vol 54 (1) ◽  
pp. 601-607 ◽  
Author(s):  
Elham Hoveizi ◽  
Somayeh Ebrahimi-Barough ◽  
Shima Tavakol ◽  
Khadije Sanamiri
Keyword(s):  
Ips Cells ◽  
In Vitro Differentiation ◽  
Human Ips Cells

1082 MYOFIBROBLASTS MODULATE THE TRANS-DIFFERENTIATION OF HEPATIC PROGENITOR CELLS IN VITRO

2011 ◽  
Vol 54 ◽  
pp. S428-S429
Author(s):  
E. Fairhall ◽  
K. Wallace ◽  
K. Charlton ◽  
M. Wright
Keyword(s):  
Progenitor Cells ◽  
Hepatic Progenitor Cells

In Vitro Insulin Production and Analysis of Pancreatic Transcription Factors in Induced Human Hepatic Progenitor Cells

2010 ◽  
Vol 12 (5) ◽  
pp. 373-378 ◽  
Author(s):  
Aleem A. Khan ◽  
Alaganuru Rajendraprasad ◽  
N. Parveen ◽  
Mahaboob V. Shaik ◽  
Santosh K. Tiwari ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Progenitor Cells ◽  
Hepatic Progenitor Cells ◽  
Insulin Production
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