Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)

2015 ◽  
pp. 97-110 ◽  
Author(s):  
Lucas V. Greder ◽  
Jason Post ◽  
James R. Dutton
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Lineage Tracing ◽  
Oct4 Expression ◽  
Induced Pluripotent

scholarly journals Biological importance of OCT transcription factors in reprogramming and development

2021 ◽  
Author(s):  
Kee-Pyo Kim ◽  
Dong Wook Han ◽  
Johnny Kim ◽  
Hans R. Schöler
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Ectopic Expression ◽  
Donor Cell ◽  
Structural Components ◽  
Oct4 Expression ◽  
Reprogramming Factors ◽  
Induced Pluripotent

AbstractEctopic expression of Oct4, Sox2, Klf4 and c-Myc can reprogram somatic cells into induced pluripotent stem cells (iPSCs). Attempts to identify genes or chemicals that can functionally replace each of these four reprogramming factors have revealed that exogenous Oct4 is not necessary for reprogramming under certain conditions or in the presence of alternative factors that can regulate endogenous Oct4 expression. For example, polycistronic expression of Sox2, Klf4 and c-Myc can elicit reprogramming by activating endogenous Oct4 expression indirectly. Experiments in which the reprogramming competence of all other Oct family members tested and also in different species have led to the decisive conclusion that Oct proteins display different reprogramming competences and species-dependent reprogramming activity despite their profound sequence conservation. We discuss the roles of the structural components of Oct proteins in reprogramming and how donor cell epigenomes endow Oct proteins with different reprogramming competences.

scholarly journals Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses

2015 ◽  
Vol 72 (23) ◽  
pp. 4671-4680 ◽  
Author(s):  
Laura Hildebrand ◽  
Petra Seemann ◽  
Andreas Kurtz ◽  
Jochen Hecht ◽  
Jörg Contzen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Lineage Tracing ◽  
Cell Targeting ◽  
Induced Pluripotent

scholarly journals Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Andreas Reimer ◽  
Aliaksei Vasilevich ◽  
Frits Hulshof ◽  
Priyalakshmi Viswanathan ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Oct4 Expression ◽  
Induced Pluripotent

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent

Derivation and Characterization of human Induced Pluripotent Stem Cells

Acta Naturae ◽  
2009 ◽  
Vol 1 (2) ◽  
pp. 91-92 ◽  
Author(s):  
M V Shutova ◽  
A N Bogomazova ◽  
M A Lagarkova ◽  
S L Kiselev
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Derivation of bovine induced pluripotent stem cells by a transposon approach

2014 ◽  
Author(s):  
Talluri Thirumala Rao ◽  
Dharmendra Kumar ◽  
Silke Glage ◽  
Wiebke Garrels ◽  
Katharina Debowski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document