scholarly journals Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix

2014 ◽  
pp. 39-60 ◽  
Author(s):  
Andres Sanz-Garcia ◽  
Miodrag Stojkovic ◽  
Carmen Escobedo-Lucea
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0129855 ◽  
Author(s):  
Chiemi Takenaka ◽  
Hiroshi Miyajima ◽  
Yusuke Yoda ◽  
Hideo Imazato ◽  
Takako Yamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Pluripotent Stem Cells ◽  
Defined Medium ◽  
Human Pluripotent Stem Cells ◽  
Controlled Growth

Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix

Biomaterials ◽  
2018 ◽  
Vol 150 ◽  
pp. 100-111 ◽  
Author(s):  
In Gul Kim ◽  
Chang-Hyun Gil ◽  
Joseph Seo ◽  
Soon-Jung Park ◽  
Ramesh Subbiah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Superresolution architecture of cornerstone focal adhesions in human pluripotent stem cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Aki Stubb ◽  
Camilo Guzmán ◽  
Elisa Närvä ◽  
Jesse Aaron ◽  
Teng-Leong Chew ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Focal Adhesions ◽  
Structural Features ◽  
Human Pluripotent Stem Cells ◽  
Actin Ring ◽  
Localisation Microscopy

Abstract While it is clear that key transcriptional programmes are important for maintaining pluripotency, the requirement for cell adhesion to the extracellular matrix remains poorly defined. Human pluripotent stem cells (hPSCs) form colonies encircled by an actin ring and large stable cornerstone focal adhesions (FA). Using superresolution two-colour interferometric photo-activated localisation microscopy, we examine the three-dimensional architecture of cornerstone adhesions and report vertical lamination of FA proteins with three main structural features distinct from previously studied focal adhesions: 1) integrin β5 and talin are present at high density, at the edges of cornerstone FA, adjacent to a vertical kank-rich protein wall, 2) vinculin localises higher than previously reported, displaying a head-above-tail orientation, and 3) surprisingly, actin and α-actinin are present in two discrete z-layers. Finally, we report that depletion of kanks diminishes FA patterning, and actin organisation within the colony, indicating a role for kanks in hPSC colony architecture.

scholarly journals Extracellular Matrix Promotes Highly Efficient Cardiac Differentiation of Human Pluripotent Stem Cells

2012 ◽  
Vol 111 (9) ◽  
pp. 1125-1136 ◽  
Author(s):  
Jianhua Zhang ◽  
Matthew Klos ◽  
Gisela F. Wilson ◽  
Amanda M. Herman ◽  
Xiaojun Lian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cardiac Differentiation ◽  
Highly Efficient
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