scholarly journals Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

2014 ◽  
pp. 73-85 ◽  
Author(s):  
Stephen Meek ◽  
Linda Sutherland ◽  
Tom Burdon
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Embryonic Stem

scholarly journals Similar Population of CD133+ and DDX4+ VSEL-Like Stem Cells Sorted from Human Embryonic Stem Cell, Ovarian, and Ovarian Cancer Ascites Cell Cultures: The Real Embryonic Stem Cells?

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 706 ◽  
Author(s):  
Irma Virant-Klun ◽  
Petra Skerl ◽  
Srdjan Novakovic ◽  
Eda Vrtacnik-Bokal ◽  
Spela Smrkolj
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Recurrent Ovarian Cancer

A population of small stem cells with diameters of up to 5 μm resembling very small embryonic-like stem cells (VSELs) were sorted from human embryonic stem cell (hESC) cultures using magnetic-activated cell sorting (MACS) based on the expression of a stem-cell-related marker prominin-1 (CD133). These VSEL-like stem cells had nuclei that almost filled the whole cell volume and expressed stem-cell-related markers (CD133, SSEA-4) and markers of germinal lineage (DDX4/VASA, PRDM14). They were comparable to similar populations of small stem cells sorted from cell cultures of normal ovaries and were the predominant cells in ascites of recurrent ovarian cancer. The sorted populations of CD133+ VSEL-like stem cells were quiescent in vitro, except for ascites, and were highly activated after exposure to valproic acid and follicle-stimulating hormone (FSH), indicating a new tool to study these cells in vitro. These VSEL-like stem cells spontaneously formed clusters resembling tumour-like structures or grew into larger, oocyte-like cells and were differentiated in vitro into adipogenic, osteogenic and neural lineages after sorting. We propose the population of VSEL-like stem cells from hESC cultures as potential original embryonic stem cells, which are present in the human embryo, persist in adult human ovaries from the embryonic period of life and are involved in cancer manifestation.

scholarly journals PDGFRα + Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors

2017 ◽  
Vol 8 (2) ◽  
pp. 318-333 ◽  
Author(s):  
Antonio Lo Nigro ◽  
Anchel de Jaime-Soguero ◽  
Rita Khoueiry ◽  
Dong Seong Cho ◽  
Giorgia Maria Ferlazzo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Embryonic Stem ◽  
Primitive Endoderm

Derivation of goat embryonic stem cell-like cell lines from in vitro produced parthenogenetic blastocysts

2013 ◽  
Vol 113 (1) ◽  
pp. 145-153 ◽  
Author(s):  
Arun Kumar De ◽  
Shweta Garg ◽  
Dinesh Kumar Singhal ◽  
Hrudananda Malik ◽  
Ayan Mukherjee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem

In vitro recapitulation of the urea cycle using murine embryonic stem cell-derived in vitro liver model

Amino Acids ◽  
2013 ◽  
Vol 45 (6) ◽  
pp. 1343-1351 ◽  
Author(s):  
Miho Tamai ◽  
Mami Aoki ◽  
Akihito Nishimura ◽  
Koji Morishita ◽  
Yoh-ichi Tagawa
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Urea Cycle ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cell ◽  
Liver Model

scholarly journals Pituitary adenylyl cyclase-activating peptide counteracts hedgehog-dependent motor neuron production in mouse embryonic stem cell cultures

2011 ◽  
Vol 89 (9) ◽  
pp. 1363-1374 ◽  
Author(s):  
Megumi Hirose ◽  
Pawel Niewiadomski ◽  
Gary Tse ◽  
Gloria C. Chi ◽  
Hongmei Dong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Motor Neuron ◽  
Adenylyl Cyclase ◽  
Cell Cultures ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell

scholarly journals BCL-XL Mediates the Strong Selective Advantage of a 20q11.21 Amplification Commonly Found in Human Embryonic Stem Cell Cultures

2013 ◽  
Vol 1 (5) ◽  
pp. 379-386 ◽  
Author(s):  
Stuart Avery ◽  
Adam J. Hirst ◽  
Duncan Baker ◽  
Chin Yan Lim ◽  
Sharmini Alagaratnam ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Selective Advantage

In vitro assessing the risk of drug-induced cardiotoxicity by embryonic stem cell-based biosensor

2011 ◽  
Vol 155 (1) ◽  
pp. 214-219 ◽  
Author(s):  
Qingjun Liu ◽  
Hui Yu ◽  
Zhou Tan ◽  
Hua Cai ◽  
Weiwei Ye ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Drug Induced

In vitro differentiation of retinal ganglion-like cells from embryonic stem cell derived neural progenitors

2009 ◽  
Vol 380 (2) ◽  
pp. 230-235 ◽  
Author(s):  
Balusamy Jagatha ◽  
Mundackal S. Divya ◽  
Rajendran Sanalkumar ◽  
Chandrasekharan L. Indulekha ◽  
Sasidharan Vidyanand ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
Retinal Ganglion ◽  
In Vitro Differentiation
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