Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intramuscular and Systemic Delivery

2013 ◽  
pp. 177-193 ◽  
Author(s):  
Boris Odintsov ◽  
Ju Lan Chun ◽  
Suzanne E. Berry
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Fluorescent Microscopy ◽  
Superparamagnetic Iron Oxide ◽  
Whole Body ◽  
Systemic Delivery ◽  
Whole Body Mri

scholarly journals Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 484
Author(s):  
Yue Gao ◽  
Anna Jablonska ◽  
Chengyan Chu ◽  
Piotr Walczak ◽  
Miroslaw Janowski
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Extracellular Vesicles ◽  
Superparamagnetic Iron Oxide ◽  
Cell Labeling ◽  
Oxide Nanoparticles ◽  
Stem Cell Delivery ◽  
Cellular Labeling

Rapidly ageing populations are beset by tissue wear and damage. Stem cell-based regenerative medicine is considered a solution. Years of research point to two important aspects: (1) the use of cellular imaging to achieve sufficient precision of therapeutic intervention, and the fact that (2) many therapeutic actions are executed through extracellular vesicles (EV), released by stem cells. Therefore, there is an urgent need to interrogate cellular labels in the context of EV release. We studied clinically applicable cellular labels: superparamagnetic iron oxide nanoparticles (SPION), and radionuclide detectable by two main imaging modalities: MRI and PET. We have demonstrated effective stem cell labeling using both labels. Then, we obtained EVs from cell cultures and tested for the presence of cellular labels. We did not find either magnetic or radioactive labels in EVs. Therefore, we report that stem cells do not lose labels in released EVs, which indicates the reliability of stem cell magnetic and radioactive labeling, and that there is no interference of labels with EV content. In conclusion, we observed that direct cellular labeling seems to be an attractive approach to monitoring stem cell delivery, and that, importantly, labels neither locate in EVs nor affect their basic properties.

Controllable labelling of stem cells with a novel superparamagnetic iron oxide–loaded cationic nanovesicle for MR imaging

2012 ◽  
Vol 22 (11) ◽  
pp. 2328-2337 ◽  
Author(s):  
Ruo Mi Guo ◽  
Nuo Cao ◽  
Fang Zhang ◽  
Yi Ru Wang ◽  
Xue Hua Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Mr Imaging ◽  
Superparamagnetic Iron Oxide

Superparamagnetic iron oxide labeling of neural stem cells and 4.7T MRI tracking in vivo and in vitro

2007 ◽  
Vol 27 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Wenzhen Zhu ◽  
Xiang Li ◽  
Zhouping Tang ◽  
Suiqiang Zhu ◽  
Jianpin Qi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Neural Stem Cells ◽  
Superparamagnetic Iron Oxide
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