Cytological, Physiological and Biochemical Aspectsof Somatic Embryo Formation in Flax

2006 ◽  
pp. 235-245
Author(s):  
Anna Pret'ová ◽  
Jozef Šamaj ◽  
Bohuš Obert
Keyword(s):  
Somatic Embryo ◽  
Embryo Formation ◽  
Physiological And Biochemical

scholarly journals Pengaruh Sitokinin, Jenis Eksplan, dan Genotipe terhadap Embriogenesis Somatik Kakao

2016 ◽  
Vol 3 (2) ◽  
pp. 71
Author(s):  
Nur Ajijah ◽  
RR. Sri Hartati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Theobroma Cacao ◽  
Somatic Embryos ◽  
Interaction Effects ◽  
Embryo Formation ◽  
Primary Somatic Embryogenesis ◽  
Primary Somatic Embryos

<p><em>Information on the effect of cytokinins on cacao (</em>Theobroma cacao<em> L.) primary somatic embryogenesis and its interaction with explant types and genotypes is not yet known. This study aimed to evaluate the effect of cytokinins and its interaction with explant types and genotypes on cacao somatic embryogenesis. The study was conducted at tissue culture laboratory of IAARD, Bogor from April until December 2012 and October 2014 until February 2016. Three types of cytokinins i.e. kinetin (0.58, 1.16, and 2.32 </em><em>μ</em><em>M), thidiazuron (0.01, 0.02, and 0.04 </em><em>μ</em><em>M) and benzylaminopurine (0.55, 1.11, and 2.22 </em><em>μ</em><em>M) in combination with 9 </em><em>μ</em><em>M 2,4-D were tested for their effectiveness in inducing somatic embryogenesis from petals and staminoid explants of Cimanggu 1 genotype. Furthermore, three levels of kinetin (0.58, 1.16, and 2.32 </em><em>μ</em><em>M</em><em>) also in combination with 9 </em><em>μ</em><em>M 2,4-D were evaluated for their influences on the somatic embryogenesis from petals and staminoid explants of three cacao genotypes i.e. Sulawesi 02, ICCRI 04 and Cimanggu 3. The result demonstrated that 2.32 </em><em>μ</em><em>M kinetin and staminoids explant were more effective to induce cacao somatic embryogenesis of Cimanggu 1 genotype (7%, 0.23 embryos/explant). Additionally, there were interaction effects between the level of kinetin with explant types and genotype on the percentage of explants forming embryo at 12 weeks after culture. The highest percentage of somatic embryo formation was shown by ICCRI 04 genotype with the use of petals explant and a kinetin level of 1.16 </em><em>μ</em><em>M (31.85%), but not significantly different from the level of kinetin 2.23 </em><em>μ</em><em>M (25.55%). The formation of primary somatic embryos of cacao is largely determined by the type and level of cytokinins, type of explant, and genotype.</em></p>

Comparing carbohydrate status during norway spruce seed development and somatic embryo formation

2001 ◽  
Vol 37 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Monika Gösslová ◽  
Hana Svobodová ◽  
Helena Lipavská ◽  
Jana Albrechtová ◽  
Dick Vreugdenhil
Keyword(s):  
Somatic Embryo ◽  
Norway Spruce ◽  
Seed Development ◽  
Embryo Formation ◽  
Carbohydrate Status

scholarly journals Peningkatan Efisiensi Media Pembentukan Embrio Somatik Kakao Melalui Penggunaan Gula Pasir

2020 ◽  
Vol 7 (1) ◽  
pp. 29
Author(s):  
Nur Ajijah ◽  
Sri Hartati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
Low Cost ◽  
Block Design ◽  
Carbon Sources ◽  
Experimental Unit ◽  
Culture Methods ◽  
Average Percentage ◽  
Embryo Formation

<p><em>Availability of inexpensive and efficient tissue culture methods is needed in the development of mass propagation methods. The efficiency can be obtained through the use of low cost media components such as granulated sugar. The study aimed to determine the efficiency of cacao somatic embryo development medium using granulated sugar and its effect on somatic embryo formation. The study was conducted at Tissue Culture Laboratory for Superior Seed Development, IAARD, Bogor, from March to October 2016. Formation and maturation of somatic embryos was conducted using DKW medium without growth regulators. </em><em>The treatments tested were carbon sources in the media (laboratory grade sucrose; a mixture of sucrose and granulated sugar with a ratio of 3:1, 2:2 and 1:3; granulated sugar) and cacao genotypes (Sca 6 and ICCRI 4). The study used in a randomized complete block design in factorial 2 favtors with 8 replications, which consisted of 10 explants per experimental unit. The results showed that carbon sources and genotypes significantly affected somatic embryos formation, whereas the interaction effect was not significant. The use of granulated sugar 30 g/l produced approximately 35.3% of somatic embryo formation and 3.5 somatic embryos per explant, which is not significantly different from sucrose 30 g/l (31.1% and 4.1), whereas the mixture of sucrose and granulated sugar produced the lowest average percentage of somatic embryo formation and the number of somatic embryos per explant (20.3%, 18.8%, 17.8% and 1.5; 2.2; 2.8 respectively). The low-cost somatic embryogenesis method was successfully developed with an efficiency of 98.8% per liter of media.</em></p>

Recent Advances on Genetic and Physiological Bases of In Vitro Somatic Embryo Formation

2016 ◽  
pp. 47-85 ◽  
Author(s):  
Maria Maddalena Altamura ◽  
Federica Della Rovere ◽  
Laura Fattorini ◽  
Simone D’Angeli ◽  
Giuseppina Falasca
Keyword(s):  
Somatic Embryo ◽  
Embryo Formation ◽  
Recent Advances

Morphological and histological changes during somatic embryo formation from coconut plumule explants

2006 ◽  
Vol 42 (1) ◽  
pp. 19-25 ◽  
Author(s):  
L. Sáenz ◽  
A. Azpeitia ◽  
B. Chuc-Armendariz ◽  
J. L. Chan ◽  
J. -L. Verdeil ◽  
...  
Keyword(s):  
Somatic Embryo ◽  
Histological Changes ◽  
Embryo Formation
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