Storage Proteins and Peroxidase Activity During Zygotic and Somatic Embryogenesis of Firs (Abies sp.)

2006 ◽  
pp. 1-14
Author(s):  
A. Kormut'ák ◽  
B. Vooková
Keyword(s):  
Somatic Embryogenesis ◽  
Peroxidase Activity ◽  
Storage Proteins

Somatic embryogenesis from stamen filaments of Aesculus flava Sol. and peroxidase activity during the transition from friable to embryogenic callus

2019 ◽  
Vol 247 ◽  
pp. 362-372 ◽  
Author(s):  
Snežana Zdravković-Korać ◽  
Ljiljana Tubić ◽  
Nina Devrnja ◽  
Dušica Ćalić ◽  
Jelena Milojević ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Peroxidase Activity ◽  
Embryogenic Callus

scholarly journals Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species

Dendrobiology ◽  
2012 ◽  
Vol 69 ◽  
pp. 77-86 ◽  
Author(s):  
Teresa Hazubska-Przybył ◽  
Ewelina Ratajczak ◽  
Ewa Marzena Kalemba ◽  
Krystyna Bojarczuk
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Peroxidase Activity ◽  
Guaiacol Peroxidase ◽  
Induction Phase ◽  
Picea Species

Conifer zygotic embryogenesis, somatic embryogenesis, and seed germination: Biochemical and molecular advances

1994 ◽  
Vol 4 (4) ◽  
pp. 357-384 ◽  
Author(s):  
Santosh Misra
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Seed Germination ◽  
Storage Protein ◽  
Storage Proteins ◽  
Experimental System ◽  
Seed Storage Proteins ◽  
Zygotic Embryogenesis

AbstractThe development of techniques for somatic embryogenesis in conifers has led to rapid advances in the ability to culture conifer tissuein vitro.Somatic embryogenesis now provides a means to clonally propagate commercially valuable conifers of several species and offers anin vitroexperimental system for studying embryogenesis. As a result of these developments, the conifer zygotic and somatic embryo system has recently attracted the attention not only of the cell biologist but also of biochemists and molecular biologists. In this review I have attempted to consolidate the recent information on ultrastructure, biochemical and molecular aspects of conifer zygotic and somatic embryogenesis. In the first section, salient features of zygotic embryogenesis are highlighted followed by biochemical and ultrastructural characterization of development with reference to storage reserve deposition. In the second section, detailed characterization of seed storage proteins, changes in gene expression with emphasis on storage protein genes and Lea genes during zygotic and somatic embryogenesis are described. Effect of culture treatments such as abscisic acid, osmoticum and desiccation on storage protein gene expression in somatic embryos is discussed. Based on these studies, comparisons are presented between angiosperm and gymnosperm embryogeny regarding the unique morphological and developmental patterns of conifer embryogenesis. Finally, a brief discussion of recent progress in biochemical and molecular characterization of conifer seed germination is presented.

scholarly journals Ontogeny of Grape Somatic Embryos

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 587c-587
Author(s):  
D.J. Gray ◽  
K.A. Labeau ◽  
C.M. Benton
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Storage Proteins ◽  
Direct Somatic Embryogenesis ◽  
Zygotic Embryogenesis ◽  
Culture Vessel ◽  
Embryogenic Cell ◽  
Meristem Development

The development of grape somatic embryos (Vitis vinifera cv. Thompson Seedless) was studied using high-resolution light microscopy and scanning electron microscopy. Somatic embryos develop either from discrete embryogenic cell clusters (indirect somatic embryogenesis) or from previously formed somatic embryos (direct somatic embryogenesis). In both instances, embryo development begins when a small, isodiametric, densely cytoplasmic cell undergoes a series of organized divisions, which are identical to those observed during zygotic embryogenesis. Developing embryos pass through recognizable embryonic stages, remaining white and opaque through maturity. Upon germination, embryos begin to enlarge, become yellow, then green, and develop into morphologically correct plants. The cells of somatic embryos contain little starch, but abundant storage proteins. However, lipids comprise the primary storage compound. Some developmental abnormalities occur during embryogenesis, including overly enlarged hypocotyls and fewer or more than two cotyledons. In addition, relatively few somatic embryos grow into plants primarily due to inadequate shoot apical meristem development. These abnormalities are best attributed to inadequacies of the in vitro environment of medium in a culture vessel when compared to the in vivo environment of a seed.

scholarly journals PEROXIDASE ACTIVITY DIFFERS IN SWEET CORN GENOTYPES

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 434c-434
Author(s):  
J.K. Collins ◽  
C. Biles ◽  
E.V. Wann ◽  
P. Perkins-Veazie
Keyword(s):  
Peroxidase Activity ◽  
Sweet Corn ◽  
Storage Proteins ◽  
Storage Duration ◽  
Native Page ◽  
Banding Patterns ◽  
Peroxidase Isozyme ◽  
Peroxidase Isozymes ◽  
Total Peroxidase Activity ◽  
And Storage

Increased peroxidase activity is used to predict development of off-flavor in frozen sweet corn. However, peroxidase activity was not indicative of flavor changes in frozen supersweet (sh2) or sugar enhanced (sul/se) sweet corn genotypes. These results suggested an inactivation or absence of certain peroxidase isozymes. Frozen `Florida Staysweet' (sh2), `Merit' (sul), and `Bodacious' (sul/se) kernels were cut from cobs after 0 and 12 months of storage. Proteins extracted from acetone powders were separated by isoelectric focusing (IEF) and Native-PAGE. Banding patterns differed according to cultivar and storage duration. All cultivars contained a peroxidase isozyme having a molecular weight of 99 kD and pI of 4.5. The sul/se and su2 cultivars expressed an additional peroxidase band of 17.9 kD. An additional peroxidase isozyme (pI 5.0) appeared after 12 months of storage in the sul cultivar. This isozyme did not appear in sul/se or sh2 and is a possible marker for predicting off-flavor in corn. This isozyme may also catalyze off-flavor reactions in sul corn genotypes. Although changes in total peroxidase activity may not predict flavor loss in all genotypes, certain peroxidase isozymes may be useful in predicting and catalyzing off-flavor reactions in sul corn cultivars.

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