scholarly journals Meiotic Recombination in Schizosaccharomyces pombe: A Paradigm for Genetic and Molecular Analysis

2007 ◽  
pp. 195-230 ◽  
Author(s):  
Gareth Cromie ◽  
Gerald R. Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Molecular Analysis ◽  
Meiotic Recombination

scholarly journals Active and inactive transplacement of the M26 recombination hotspot in Schizosaccharomyces pombe.

Genetics ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 33-48
Author(s):  
J B Virgin ◽  
J Metzger ◽  
G R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Plasmid Dna ◽  
Context Effect ◽  
Intragenic Recombination ◽  
Multicopy Plasmid ◽  
Chromosomal Locus ◽  
Sequence Element ◽  
Recombination Hotspot ◽  
Recombination Hotspots

Abstract The ade6-M26 mutation of the fission yeast Schizosaccharomyces pombe creates a meiotic recombination hotspot that elevates ade6 intragenic recombination approximately 10-15-fold. A heptanucleotide sequence including the M26 point mutation is required but not sufficient for hotspot activity. We studied the effects of plasmid and chromosomal context on M26 hotspot activity. The M26 hotspot was inactive on a multicopy plasmid containing M26 embedded within 3.0 or 5.9 kb of ade6 DNA. Random S. pombe genomic fragments totaling approximately 7 Mb did not activate the M26 hotspot on a plasmid. M26 hotspot activity was maintained when 3.0-, 4.4-, and 5.9-kb ade6-M26 DNA fragments, with various amounts of non-S. pombe plasmid DNA, were integrated at the ura4 chromosomal locus, but only in certain configurations relative to the ura4 gene and the cointegrated plasmid DNA. Several integrations created new M26-independent recombination hotspots. In all cases the non-ade6 DNA was located > 1 kb from the M26 site, and in some cases > 2 kb. Because the chromosomal context effect was transmitted over large distances, and did not appear to be mediated by a single discrete DNA sequence element, we infer that the local chromatin structure has a pronounced effect on M26 hotspot activity.

Region‐specific meiotic recombination in Schizosaccharomyces pombe : the rec11 gene

1997 ◽  
Vol 23 (5) ◽  
pp. 869-878 ◽  
Author(s):  
Ywan Feng Li ◽  
Masayuki Numata ◽  
Wayne P. Wahls ◽  
Gerald R. Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination

Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast

1993 ◽  
Vol 23 (4) ◽  
pp. 295-304 ◽  
Author(s):  
Doug Pittman ◽  
Wei Lu ◽  
Robert E. Malone
Keyword(s):  
Molecular Analysis ◽  
Meiotic Recombination ◽  
Recombination Gene

scholarly journals Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.

Genetics ◽  
1988 ◽  
Vol 119 (3) ◽  
pp. 491-497
Author(s):  
A S Ponticelli ◽  
E P Sena ◽  
G R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Base Pair ◽  
Initiation Site ◽  
Mitotic Recombination ◽  
Physical Analysis ◽  
Nucleotide Change ◽  
Recombination Hotspot ◽  
Base Pair Mismatch

Abstract The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G----T nucleotide change, that M26 stimulated recombination within ade6 but not at other distinct loci, and that M26 stimulated meiotic but not mitotic recombination. In addition, M26 stimulated recombination within ade6 when M26 is homozygous; this result demonstrates that a base-pair mismatch at the M26 site was not required for the stimulation. These results are consistent with the ade6-M26 mutation creating a meiotic recombination initiation site.

Sequence requirement of the ade6-4095 meiotic recombination hotspot in Schizosaccharomyces pombe

Genetica ◽  
2017 ◽  
Vol 146 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Steven J. Foulis ◽  
Kyle R. Fowler ◽  
Walter W. Steiner
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Recombination Hotspot

scholarly journals Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe

2005 ◽  
Vol 48 (5) ◽  
pp. 323-333 ◽  
Author(s):  
Eveline Doll ◽  
Monika Molnar ◽  
Yasushi Hiraoka ◽  
Jürg Kohli
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Recombination Gene

scholarly journals Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe

Chromosoma ◽  
2006 ◽  
Vol 115 (4) ◽  
pp. 330-340 ◽  
Author(s):  
Alexander Lorenz ◽  
Anna Estreicher ◽  
Jürg Kohli ◽  
Josef Loidl
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Recombination Proteins ◽  
Linear Elements

scholarly journals Five RecA-like Proteins of Schizosaccharomyces pombe Are Involved in Meiotic Recombination

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1031-1043
Author(s):  
A L Grishchuk ◽  
J Kohli
Keyword(s):  
Escherichia Coli ◽  
Schizosaccharomyces Pombe ◽  
Meiotic Recombination ◽  
Sequence Similarity ◽  
Intragenic Recombination ◽  
Wild Type ◽  
Spore Viability ◽  
Viability Analysis ◽  
Recombination Protein ◽  
Recombination Gene

Abstract The genome of Schizosaccharomyces pombe contains five genes that code for proteins with sequence similarity to the Escherichia coli recombination protein RecA: rad51+, rhp55+, rhp57+, rlp1+, and dmc1+. We analyzed the effect of deletion of each of these genes on meiotic recombination and viability of spores. Meiotic recombination levels were different from wild type in all recA-related mutants in several genetic intervals, suggesting that all five RecA homologs of S. pombe are required for normal levels of meiotic recombination. Spore viability was reduced in rad51, rhp55, and rhp57 mutants, but not in rlp1 and dmc1. It is argued that reduction of crossover is not the only cause for the observed reduction of spore viability. Analysis of double and triple mutants revealed that Rad51 and Dmc1 play major and partially overlapping roles in meiotic recombination, while Rhp55, Rhp57, and Rlp1 play accessory roles. Remarkably, deletion of Rlp1 decreases the frequency of intergenic recombination (crossovers), but increases intragenic recombination (gene conversion). On the basis of our results, we present a model for the involvement of five RecA-like proteins of S. pombe in meiotic recombination and discuss their respective roles.

scholarly journals Nonrandom Homolog Segregation at Meiosis I in Schizosaccharomyces pombe Mutants Lacking Recombination

Genetics ◽  
2003 ◽  
Vol 163 (3) ◽  
pp. 857-874 ◽  
Author(s):  
Luther Davis ◽  
Gerald R Smith
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Sister Chromatid ◽  
Meiotic Recombination ◽  
Meiotic Division ◽  
General Feature ◽  
Homologous Chromosomes ◽  
Chromatid Cohesion ◽  
Homozygous Diploid ◽  
Reductional Division ◽  
Meiosis I

Abstract Physical connection between homologous chromosomes is normally required for their proper segregation to opposite poles at the first meiotic division (MI). This connection is generally provided by the combination of reciprocal recombination and sister-chromatid cohesion. In the absence of meiotic recombination, homologs are predicted to segregate randomly at MI. Here we demonstrate that in rec12 mutants of the fission yeast Schizosaccharomyces pombe, which are devoid of meiosis-induced recombination, homologs segregate to opposite poles at MI 63% of the time. Residual, Rec12-independent recombination appears insufficient to account for the observed nonrandom homolog segregation. Dyad asci are frequently produced by rec12 mutants. More than half of these dyad asci contain two viable homozygous-diploid spores, the products of a single reductional division. This set of phenotypes is shared by other S. pombe mutants that lack meiotic recombination, suggesting that nonrandom MI segregation and dyad formation are a general feature of meiosis in the absence of recombination and are not peculiar to rec12 mutants. Rec8, a meiosis-specific sister-chromatid cohesin, is required for the segregation phenotypes displayed by rec12 mutants. We propose that S. pombe possesses a system independent of recombination that promotes homolog segregation and discuss possible mechanisms.

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