Abiotic and Biological Technologies for the Remediation of Phenylurea Herbicides in Soils

2021 ◽  
Author(s):  
Alba Lara-Moreno ◽  
Jaime Villaverde ◽  
Marina Rubio-Bellido ◽  
Fernando Madrid ◽  
Esmeralda Morillo
Keyword(s):  
Phenylurea Herbicides

scholarly journals A Novel Hydrolase Identified by Genomic-Proteomic Analysis of Phenylurea Herbicide Mineralization by Variovorax sp. Strain SRS16

2011 ◽  
Vol 77 (24) ◽  
pp. 8754-8764 ◽  
Author(s):  
Karolien Bers ◽  
Baptiste Leroy ◽  
Philip Breugelmans ◽  
Pieter Albers ◽  
Rob Lavigne ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Krebs Cycle ◽  
Divergent Evolution ◽  
Phenylurea Herbicides ◽  
Transcription Analysis ◽  
Content Type ◽  
First Report ◽  
Phenylurea Herbicide ◽  
Hydrolyzing Enzymes ◽  
Krebs Cycle Intermediates

ABSTRACTThe soil bacterial isolateVariovoraxsp. strain SRS16 mineralizes the phenylurea herbicide linuron. The proposed pathway initiates with hydrolysis of linuron to 3,4-dichloroaniline (DCA) andN,O-dimethylhydroxylamine, followed by conversion of DCA to Krebs cycle intermediates. Differential proteomic analysis showed a linuron-dependent upregulation of several enzymes that fit into this pathway, including an amidase (LibA), a multicomponent chloroaniline dioxygenase, and enzymes associated with a modified chlorocatecholortho-cleavage pathway. Purified LibA is a monomeric linuron hydrolase of ∼55 kDa with aKmand aVmaxfor linuron of 5.8 μM and 0.16 nmol min−1, respectively. This novel member of the amidase signature family is unrelated to phenylurea-hydrolyzing enzymes from Gram-positive bacteria and lacks activity toward other tested phenylurea herbicides. Orthologues oflibAare present in all other tested linuron-degradingVariovoraxstrains with the exception ofVariovoraxstrains WDL1 and PBS-H4, suggesting divergent evolution of the linuron catabolic pathway in differentVariovoraxstrains. The organization of the linuron degradation genes identified in the draft SRS16 genome sequence indicates that gene patchwork assembly is at the origin of the pathway. Transcription analysis suggests that a catabolic intermediate, rather than linuron itself, acts as effector in activation of the pathway. Our study provides the first report on the genetic organization of a bacterial pathway for complete mineralization of a phenylurea herbicide and the first report on a linuron hydrolase in Gram-negative bacteria.

The design of postemergence phenylurea herbicides using physiochemical parameters and structure-activity analyses

1983 ◽  
Vol 31 (2) ◽  
pp. 260-264 ◽  
Author(s):  
Barrington Cross ◽  
Peggy P. Hoffman ◽  
George T. Santora ◽  
David M. Spatz ◽  
Andrew R. Templeton
Keyword(s):  
Phenylurea Herbicides ◽  
Physiochemical Parameters ◽  
Structure Activity

Mixed Immunosorbent for Selective On-line Trace Enrichment and Liquid Chromatography of Phenylurea Herbicides in Environmental Waters

The Analyst ◽  
1997 ◽  
Vol 122 (10) ◽  
pp. 1113-1118 ◽  
Author(s):  
A. Martin-Esteban ◽  
P. Fernández ◽  
A. Martin-Esteban ◽  
D. Stevenson ◽  
C. Cámara
Keyword(s):  
Liquid Chromatography ◽  
Environmental Waters ◽  
Phenylurea Herbicides ◽  
Trace Enrichment ◽  
On Line ◽  
Line Trace

Extraction of eight triazine and phenylurea herbicides in yogurt by ionic liquid foaming-based solvent floatation

2012 ◽  
Vol 1222 ◽  
pp. 22-28 ◽  
Author(s):  
Na Li ◽  
Rui Zhang ◽  
Li Nian ◽  
Ruibing Ren ◽  
Yeqiang Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Phenylurea Herbicides

Alkaline and Neutral Hydrolysis of Four Phenylurea Herbicides

1996 ◽  
Vol 64 (2) ◽  
pp. 123-134 ◽  
Author(s):  
D. Sabali[ubar]nas ◽  
J. Ellington ◽  
R. Lekevičius
Keyword(s):  
Phenylurea Herbicides ◽  
Hydrolysis Of
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