Knee Cartilage Regeneration with Umbilical Cord Mesenchymal Stem Cells Embedded in Collagen Scaffold Using Dry Arthroscopy Technique

2017 ◽  
pp. 113-122 ◽  
Author(s):  
B. Sadlik ◽  
G. Jaroslawski ◽  
D. Gladysz ◽  
M. Puszkarz ◽  
M. Markowska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Collagen Scaffold ◽  
Cartilage Regeneration ◽  
Knee Cartilage

Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells

2015 ◽  
Vol 15 (11) ◽  
pp. 1541-1552 ◽  
Author(s):  
Michail E Klontzas ◽  
Eustathios I Kenanidis ◽  
Manolis Heliotis ◽  
Eleftherios Tsiridis ◽  
Athanasios Mantalaris
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Cartilage Regeneration ◽  
And Cartilage

scholarly journals New trends for knee cartilage regeneration: from cell-free scaffolds to mesenchymal stem cells

2012 ◽  
Vol 5 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Elizaveta Kon ◽  
Giuseppe Filardo ◽  
Alice Roffi ◽  
Luca Andriolo ◽  
Maurilio Marcacci
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Regeneration ◽  
Knee Cartilage

scholarly journals Articular chondrocyte derived exosomes promote cartilage differentiation of human umbilical cord mesenchymal stem cells by activation of autophagy

2020 ◽  
Author(s):  
Ke Ma ◽  
Bo Zhu ◽  
Zetao Wang ◽  
Peian Cai ◽  
Mingwei He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Phenotypic Expression ◽  
Cartilage Regeneration ◽  
Human Umbilical Cord ◽  
Articular Chondrocyte

Abstract Background Umbilical cord mesenchymal stem cells (HUCMSCs)-based therapies were previously predicated in cartilage regeneration due to the chondrogenic potential of MSCs. However, chondrogenic differentiation of HUMSCs is limited by administration of growth factors like TGF-β that may cause cartilage hypertrophy. It has been reported the exosomes could modulate phenotypic expression of stem cells. However, the role of human chondrogenic derived exosomes (C-EXO) in chondrogenic differentiation of HUCMSCs has not been reported. Results In this study, we successfully isolated chondrocyte-derived exosomes (C-EXO) from human multi-finger cartilage and found that C-EXO efficiently promoted the proliferation and chondrogenic differentiation of HUCMSCs, evidenced by highly expressed aggrecan (ACAN), COL2A and SOX-9. Also, the expression of the fibrotic marker, COL1A and hypertrophic marker, COL10, was significantly lower than that induced by TGF-β. In vivo, stimulation of C-EXO accelerated HUCMSCs-mediated cartilage repair in rabbit models. Furthermore, C-EXO led to increasing autophagosomes during the process of chondrogenic differentiation, indicating that C-EXO promoted cartilage regeneration might be through the activation of autophagy. Conclusions This study suggests that C-EXO has an essential role in fostering chondrogenic differentiation and proliferation of HUCMSCs, which may be a stable supply for articular cartilage repair.

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