Inhibition of Urease Enzyme Production and some Other Virulence Factors Expression in Proteus mirabilis by N-Acetyl Cysteine and Dipropyl Disulphide

Proteus mirabilisis a common human pathogen causing recurrent or persistent urinary tract infections (UTIs). The underlying mechanisms forP. mirabilisto establish UTIs are not fully elucidated. In this study, we showed that loss of the sigma factor E (RpoE), mediating extracytoplasmic stress responses, decreased fimbria expression, survival in macrophages, cell invasion, and colonization in mice but increased the interleukin-8 (IL-8) expression of urothelial cells and swarming motility. This is the first study to demonstrate that RpoE modulated expression of MR/P fimbriae by regulatingmrpI, a gene encoding a recombinase controlling the orientation of MR/P fimbria promoter. By real-time reverse transcription-PCR, we found that the IL-8 mRNA amount of urothelial cells was induced significantly by lipopolysaccharides extracted fromrpoEmutant but not from the wild type. These RpoE-associated virulence factors should be coordinately expressed to enhance the fitness ofP. mirabilisin the host, including the avoidance of immune attacks. Accordingly,rpoEmutant-infected mice displayed more immune cell infiltration in bladders and kidneys during early stages of infection, and therpoEmutant had a dramatically impaired ability of colonization. Moreover, it is noteworthy that urea (the major component in urine) and polymyxin B (a cationic antimicrobial peptide) can induce expression ofrpoEby the reporter assay, suggesting that RpoE might be activated in the urinary tract. Altogether, our results indicate that RpoE is important in sensing environmental cues of the urinary tract and subsequently triggering the expression of virulence factors, which are associated with the fitness ofP. mirabilis, to build up a UTI.

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Genotypic and phenotypic profiles of virulence factors and antimicrobial resistance of Proteus mirabilis isolated from chicken carcasses: potential zoonotic risk

Brazilian Journal of Microbiology ◽

10.1007/s42770-019-00086-2 ◽

2019 ◽

Vol 50 (3) ◽

pp. 685-694 ◽

Cited By ~ 4

Author(s):

Matheus Silva Sanches ◽

Ana Angelita Sampaio Baptista ◽

Marielen de Souza ◽

Maísa Fabiana Menck-Costa ◽

Vanessa Lumi Koga ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Virulence Factors ◽

Proteus Mirabilis ◽

Zoonotic Risk

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Proteus Mirabilis Virulence Factors: Review

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2021.13.01.169 ◽

2020 ◽

Vol 13 (01) ◽

Keyword(s):

Virulence Factors ◽

Proteus Mirabilis

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Potential virulence factors of Proteus bacilli

Microbiology and Molecular Biology Reviews ◽

10.1128/mmbr.61.1.65-89.1997 ◽

1997 ◽

Vol 61 (1) ◽

pp. 65-89

Author(s):

A Rózalski ◽

Z Sidorczyk ◽

K Kotełko

Keyword(s):

Amino Acid ◽

Outer Membrane ◽

Virulence Factors ◽

Human Body ◽

Proteus Mirabilis ◽

Outer Membrane Proteins ◽

Immunoglobulin A ◽

Pathogenic Microorganisms ◽

Ecological Role ◽

Opportunistic Pathogens

The object of this review is the genus Proteus, which contains bacteria considered now to belong to the opportunistic pathogens. Widely distributed in nature (in soil, water, and sewage), Proteus species play a significant ecological role. When present in the niches of higher macroorganisms, these species are able to evoke pathological events in different regions of the human body. The invaders (Proteus mirabilis, P. vulgaris, and P. penneri) have numerous factors including fimbriae, flagella, outer membrane proteins, lipopolysaccharide, capsule antigen, urease, immunoglobulin A proteases, hemolysins, amino acid deaminases, and, finally, the most characteristic attribute of Proteus, swarming growth, enabling them to colonize and survive in higher organisms. All these features and factors are described and commented on in detail. The questions important for future investigation of these facultatively pathogenic microorganisms are also discussed.

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Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice

Microbiology ◽

10.1099/mic.0.28846-0 ◽

2006 ◽

Vol 152 (7) ◽

pp. 2149-2157 ◽

Cited By ~ 19

Author(s):

Vanessa Sosa ◽

Geraldine Schlapp ◽

Pablo Zunino

Keyword(s):

Urinary Tract ◽

Virulence Factors ◽

Proteus Mirabilis ◽

Protein Patterns ◽

Wide Range ◽

Urease Production ◽

Tract Infections ◽

Different Origins

Proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (UTIs). In this study, a collection of P. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. Isolates were characterized in vitro, assessing several virulence properties that had been previously associated with P. mirabilis uropathogenicity. Swarming motility, urease production, growth in urine, outer-membrane protein patterns, ability to grow in the presence of different iron sources, haemolysin and haemagglutinin production, and the presence and expression of diverse fimbrial genes, were analysed. In order to evaluate the infectivity of the different isolates, the experimental ascending UTI model in mice was used. Additionally, the Dienes test and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay were performed to assess the genetic diversity of the isolates. The results of the present study did not show any correlation between distribution of the diverse potential urovirulence factors and isolate source. No significant correlation was observed between infectivity and the origin of the isolates, since they all similarly colonized the urinary tract of the challenged mice. Finally, all isolates showed unique ERIC-PCR patterns, indicating that the isolates were genetically diverse. The results obtained in this study suggest that the source of P. mirabilis strains cannot be correlated with pathogenic attributes, and that the distribution of virulence factors between isolates of different origins may correspond to the opportunistic nature of the organism.

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Effect of Thiophenone Compound on Motility and Caseinase Production of Aeromonas hydrophila

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2021.v05.i02.p01 ◽

2021 ◽

Vol 5 (2) ◽

pp. 40

Author(s):

Ayu Ashari Margareth Sinaga ◽

Pande Gde Sasmita Julyantotro ◽

Dewa Ayu Angga Pebriani

Keyword(s):

Enzyme Activity ◽

Virulence Factors ◽

Intercellular Communication ◽

Communication System ◽

Aeromonas Hydrophila ◽

Enzyme Production ◽

Pathogenic Bacteria ◽

Average Diameter ◽

Bacterial Virulence ◽

Fisheries Science

Motility and caseinase production are part of bacterial virulence factors which are regulated by a bacterial intercellular communication system, often called quorum sensing (QS). This study aims to determine the effect of the QS inhibitor compound thiophenone in reducing those virulence factors. This research was conducted at the Laboratory of Fisheries Science, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to March 2020. This study tested 3 different treatments with 3 repetitions for each treatment. Treatment A as control (without the addition of thiophenone), treatment B (addition of 5 ?M thiophenone), and C treatment (addition of 10 ?M thiophenone). The results showed that thiophenone compounds can inhibit the QS system of Aeromonas hydrophila. It can be seen from the significantly reduced (P<0.05) motility and caseinase enzyme activity of A. hydrophila compared to without addition of thiophenone compounds. The average diameter of the caseinase enzyme production produced in treatment A at 22 hours was 21.40±0.36 mm, in treatment B was 19.70±0.2 mm and in treatment, C was 17.87±0.05 mm. Whereas in motility, the resulting average diameter in treatment A at 22 hours was 6.57±0.61 mm, in treatment B was 5.67±0.35 mm and in treatment, C was 5.10±0.6 mm. These results indicate that the QS thiophenone inhibitor compound can reduce virulence factors, namely motility and caseinase production from pathogenic bacteria A. hydrophila. Treatment C can decrease virulence factors compared to treatment A and B.

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