Specific Adsorption of Annexin A1 on Solid Supported Membranes: A Model Study

2006 ◽  
pp. 281-302 ◽  
Author(s):  
Claudia Steinem ◽  
Andreas Janshoff
Keyword(s):  
Specific Adsorption ◽  
Annexin A1 ◽  
Supported Membranes ◽  
Model Study ◽  
Solid Supported Membranes

scholarly journals Formation of irreversibly bound annexin A1 protein domains on POPC/POPS solid supported membranes

2008 ◽  
Vol 1778 (7-8) ◽  
pp. 1601-1610 ◽  
Author(s):  
Simon Faiß ◽  
Katja Kastl ◽  
Andreas Janshoff ◽  
Claudia Steinem
Keyword(s):  
Protein Domains ◽  
Annexin A1 ◽  
Supported Membranes ◽  
Solid Supported Membranes

Kinetics and Thermodynamics of Annexin A1 Binding to Solid-Supported Membranes:  A QCM Study†

Biochemistry ◽  
2002 ◽  
Vol 41 (31) ◽  
pp. 10087-10094 ◽  
Author(s):  
Katja Kastl ◽  
Michaela Ross ◽  
Volker Gerke ◽  
Claudia Steinem
Keyword(s):  
Annexin A1 ◽  
Supported Membranes ◽  
Kinetics And Thermodynamics ◽  
Solid Supported Membranes

Phosphorylation of C-terminal polycystin-2 influences the interaction with PIGEA14: A QCM study based on solid supported membranes

2013 ◽  
Vol 437 (4) ◽  
pp. 532-537 ◽  
Author(s):  
Daniela Morick ◽  
Michaela Schatz ◽  
Raphael Hubrich ◽  
Helen Hoffmeister ◽  
Anya Krefft ◽  
...  
Keyword(s):  
Supported Membranes ◽  
Solid Supported Membranes

scholarly journals Measuring Ion Channels on Solid Supported Membranes

2009 ◽  
Vol 97 (1) ◽  
pp. 388-396 ◽  
Author(s):  
Patrick Schulz ◽  
Benjamin Dueck ◽  
Alexandre Mourot ◽  
Lina Hatahet ◽  
Klaus Fendler
Keyword(s):  
Ion Channels ◽  
Supported Membranes ◽  
Solid Supported Membranes

Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

2011 ◽  
Vol 64 (1) ◽  
pp. 31 ◽  
Author(s):  
Olga Gaiko ◽  
Ingo Janausch ◽  
Sven Geibel ◽  
Henning Vollert ◽  
Petra Arndt ◽  
...  
Keyword(s):  
Cell Lines ◽  
Organic Cation ◽  
Chinese Hamster ◽  
Organic Cation Transporter ◽  
Supported Membranes ◽  
Cho Cell ◽  
Drug Candidates ◽  
Substrate Affinities ◽  
Solid Supported Membranes

An electrophysiological assay platform based on solid supported membranes (SSM) for the organic cation transporter (OCT) is presented. Stable Chinese hamster ovary (CHO) cell lines overexpressing the human (hOCT2) and rat transporters (rOCT2) were generated and validated. Membrane preparations from the cell lines were investigated using SSM-based electrophysiology. Baculovirus transfected insect cells (HighFive and Mimic Sf9) were also tested with the same assay but yielded less than optimal results. The assays were validated by the determination of substrate affinities and inhibition by standard inhibitors. The study demonstrates the suitability of the SSM-based electrophysiological OCT assay for rapid and automatic screening of drug candidates.

Transporter Assays Using Solid Supported Membranes: A Novel Screening Platform for Drug Discovery

2006 ◽  
Vol 4 (5) ◽  
pp. 575-582 ◽  
Author(s):  
Bela Kelety ◽  
Kerstin Diekert ◽  
Joanna Tobien ◽  
Natalie Watzke ◽  
Wolfgang Dörner ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Supported Membranes ◽  
Screening Platform ◽  
Solid Supported Membranes
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