Role of the Pico-Nano-Second Temporal Dimension in STED Microscopy

2016 ◽  
pp. 311-329 ◽  
Author(s):  
Luca Lanzanò ◽  
Lorenzo Scipioni ◽  
Marco Castello ◽  
Paolo Bianchini ◽  
Giuseppe Vicidomini ◽  
...  
Keyword(s):  
Temporal Dimension ◽  
Sted Microscopy

scholarly journals Subcellular orchestration of alpha-synuclein variants in Parkinson’s disease brains revealed by 3D multicolor STED microscopy

2018 ◽  
Author(s):  
Tim E. Moors ◽  
Christina A. Maat ◽  
Daniel Niedieker ◽  
Daniel Mona ◽  
Dennis Petersen ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Lewy Bodies ◽  
Distribution Patterns ◽  
Alpha Synuclein ◽  
Label Free ◽  
Post Translational Modifications ◽  
Sted Microscopy ◽  
C Terminus

AbstractPost-translational modifications of alpha-synuclein (aSyn), particularly phosphorylation at Serine 129 (Ser129-p) and truncation of its C-terminus (CTT), have been implicated in Parkinson’s disease (PD) pathology. To gain more insight in the relevance of Ser129-p and CTT aSyn under physiological and pathological conditions, we investigated their subcellular distribution patterns in normal aged and PD brains using highly-selective antibodies in combination with 3D multicolor STED microscopy. We show that CTT aSyn localizes in mitochondria in PD patients and controls, whereas the organization of Ser129-p in a cytoplasmic network is strongly associated with pathology. Nigral Lewy bodies show an onion skin-like architecture, with a structured framework of Ser129-p aSyn and neurofilaments encapsulating CTT aSyn in their core, which displayed high content of proteins and lipids by label-free CARS microscopy. The subcellular phenotypes of antibody-labeled pathology identified in this study provide evidence for a crucial role of Ser129-p aSyn in Lewy body formation.

Eating and ageing in rural Australia: applying temporal perspectives from phenomenology to uncover meanings in older adults’ experiences

2015 ◽  
Vol 37 (4) ◽  
pp. 753-776 ◽  
Author(s):  
ALEXANDRA CLARE KING ◽  
PETER ORPIN ◽  
JESSICA WOODROFFE ◽  
KIM BOYER
Keyword(s):  
Older Adults ◽  
Temporal Dimension ◽  
Four Dimensions ◽  
Doctoral Research ◽  
Health And Wellbeing ◽  
The Past ◽  
Vivid Memories ◽  
Depth Interviews ◽  
Rural Australia

ABSTRACTNutritious and enjoyable eating experiences are important for the health and wellbeing of older adults. Social gerontology has usefully engaged with the role of time in older adults’ eating lives, considering how routines and other temporal patterns shape experiences of food, meals and eating. Building on this foundation, the paper details one set of findings from qualitative doctoral research into older adults’ experiences of food, meals and eating. Informed by phenomenological ethnography, it engages with one of four dimensions of the human lifeworld – the temporal dimension. The research involved repeated in-depth interviews, walking interviews and observation with 21 participants aged 72–90 years, living in rural Tasmania, Australia. The temporal elements of older adults’ experiences are detailed in terms of the past, present and future. The findings show that older adults have vivid memories of eating in uncertain and austere times, and these experiences have informed their food values and behaviours into old age. In the present, older adults employ several strategies for living and eating well. Simultaneously, they are oriented towards their uncertain eating futures. These findings reveal the implicit meanings in older adults’ temporal experiences of food, meals and eating, highlighting the importance of understanding older adults’ lifeworlds, and their orientation towards the future, for developing effective responses to concerns about food and eating in this age group.

The Extent of Our Abilities: The Presence, Salience, and Sociality of Affordances

2017 ◽  
Author(s):  
John Z. Elias
Keyword(s):  
Social Interaction ◽  
Human Beings ◽  
Temporal Scales ◽  
Temporal Perspective ◽  
Temporal Dimension ◽  
In Virtue Of

We, in virtue of our sociability and plasticity, are especially open to altering and developing our capacities and abilities, thereby expanding the scope of available affordances. The distinctively dynamic and extensive nature of abilities for human beings, however, raises questions concerning the ontology of affordances, given their relativity to abilities, their being relative to abilities. These questions are particularly pressing since much of the power of the concept comes from the claim that affordances are real, that they exist in some sense. Resolution of these issues, I suggest, involves taking the temporal dimension of abilities and affordances seriously, particularly in terms of interaction across multiple temporal scales. Such a temporal perspective encompasses the modulating role of motivation, as well as questions concerning the presence and salience of affordances. I end by addressing abilities as they extend into, and are extended by, social interaction and coordination, and introduce the notion of joint affordances specifically, in contrast to the sociality of affordances more generally.

Inevitability, contingency, and the epistemic significance of time

2020 ◽  
pp. 0961463X2095167
Author(s):  
Libor Benda
Keyword(s):  
Scientific Practice ◽  
Temporal Structure ◽  
Scientific Work ◽  
Scientific Development ◽  
Temporal Dimension ◽  
Theory Of Practice ◽  
Academic Time ◽  
The Impact ◽  
Epistemic Role

A considerable attention has been given recently to the analysis of the temporal dimension(s) of science and the impact of the changes therein on scientific work. One of the questions that has emerged from the rapidly growing discussion is whether and (if so) how these changes affect not only the general structural aspects of scientific practice but also the very content of scientific knowledge. In this study, I critically examine these epistemological considerations in the available body of work on scientific temporality and argue that while there has been significant progress in our understanding of the manifold temporal layers of scientific practice, the analysis of their epistemic impact has remained rather limited in certain aspects. In particular, whereas the recent studies of academic time successfully overcome the binary perspective of “fast versus slow” academia, their considerations of the epistemic role of scientific temporality in particular seem nevertheless still couched in similarly binary terms. Against this background, the study explores—in a deliberately speculative fashion—how the available investigations into the temporal structure of science can be progressively utilized and further developed so as to enable an even more complex, nonbinary understanding of the manifold ways in which scientific practice is affected by its temporal conditions. Drawing on the contingentism/inevitabilism debate in the contemporary philosophy of science, as well as on Andrew Pickering’s “mangle” theory of practice, I develop a tentative argument that the temporal structure of scientific work should be perceived as affecting not merely the speed of scientific development—whether negatively or positively—but more importantly also its direction.

scholarly journals On a Certain Research Gap in Big Data Mining for Customer Insights

2021 ◽  
Vol 11 (15) ◽  
pp. 6993
Author(s):  
Maria Mach-Król ◽  
Bartłomiej Hadasik
Keyword(s):  
Data Mining ◽  
Big Data ◽  
Big Data Analytics ◽  
Customer Behavior ◽  
Temporal Dimension ◽  
Big Data Mining ◽  
Analysis And Synthesis ◽  
Research Gap ◽  
Key Phrases

The main purpose of this paper is to provide a theoretically grounded discussion on big data mining for customer insights, as well as to identify and describe a research gap due to the shortcomings in the use of the temporal approach in big data analyzes in scientific literature sources. This article adopts two research methods. The first method is the systematic search in bibliographic repositories aimed at identifying the concepts of big data mining for customer insights. This method has been conducted in four steps: search, selection, analysis, and synthesis. The second research method is the bibliographic verification of the obtained results. The verification consisted of querying the Scopus database with previously identified key phrases and then performing trend analysis on the revealed Scopus results. The main contributions of this study are: (1) to organize knowledge on the role of advanced big data analytics (BDA), mainly big data mining in understanding customer behavior; (2) to indicate the importance of the temporal dimension of customer behavior; and (3) to identify an interesting research gap: mining of temporal big data for a complete picture of customers.

scholarly journals Retrospective economic judgments predict individual-level changes in vote preference in the US

2021 ◽  
Vol 9 (1) ◽  
pp. 272-289
Author(s):  
Hui Bai ◽  
Christopher M. Federico
Keyword(s):  
Economic Voting ◽  
Reciprocal Effects ◽  
Temporal Dimension ◽  
Individual Level ◽  
Political Preferences ◽  
The Us ◽  
Vote Preference ◽  
Level Shifts ◽  
Over Time

While many studies have investigated what predicts citizens’ vote preferences, less is known about what predicts change in citizens’ vote preferences over time. This paper focuses on the role of judgments about national economy in the recent past (i.e., “sociotropic economic retrospections”). Two longitudinal studies show that sociotropic economic retrospections (along with partisanship, ideology, and whether incumbent is running for re-election) at a given time point predict within-person changes in vote choice over time. Furthermore, cross-lagged panel analyses found that sociotropic economic retrospections and political preferences may have reciprocal effects on each other. Together, these results illustrate the temporal dimension of economic voting by suggesting that sociotropic economic retrospections not only predict votes at single points in time, but also individual-level shifts in vote preference over time. As such, the association between sociotropic economic retrospections and vote preference is more dynamic than past literature suggests.

Integrating Aesthetic and Politics: The Mawlid Celebration in the Alhambra

2018 ◽  
Author(s):  
Olga Bush
Keyword(s):  
Comparative Analysis ◽  
Aesthetic Experience ◽  
The Political ◽  
Temporal Dimension ◽  
The Hours ◽  
The Relationship

The book closes with a study of the only extant Nasrid account of the Alhambra, Ibn al-Khaṭïb's text on the mawlid, the commemoration of the birth of the Prophet. The chapter begins with a comparative analysis of mawlid celebrations in other medieval Muslim courts focusing on the role of processions and threshold spaces, such as discussed in chapter 2. Ibn al-Khaṭïb also describes a royal tent, now lost, but refrained here, in light of chapter 4, as a case of textile architecture. The key to the analysis of the text, then, is the inter-medial relationship between the temporary tent and the permanent buildings as a ceremonial setting. This temporal dimension was thematized in the "poems of the hours" recited to measure the time of the event, elucidated here in connection with the poetic figures studied in chapter 3; as an instance of the relationship between static and kinetic elements introduced in chapter 1 ; and, further, with respect to the political and ideological dimensions of the ceremonial. Ibn al-Khaṭïb's account thus testifies to the inter-medial conception of space: an integrated aesthetic experience of architecture, poetry and textiles in the court ceremonial of the Alhambra.

scholarly journals Exploring the Release of Toxic Oligomers from α-Synuclein Fibrils with Antibodies and STED Microscopy

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 431
Author(s):  
Alessandra Bigi ◽  
Emilio Ermini ◽  
Serene W. Chen ◽  
Roberta Cascella ◽  
Cristina Cecchi
Keyword(s):  
Conformational Changes ◽  
De Novo ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Phospholipid Bilayer ◽  
Sted Microscopy ◽  
Cell Dysfunction ◽  
Intrinsically Disordered ◽  
Neuronal Membranes

α-Synuclein (αS) is an intrinsically disordered and highly dynamic protein involved in dopamine release at presynaptic terminals. The abnormal aggregation of αS as mature fibrils into intraneuronal inclusion bodies is directly linked to Parkinson’s disease. Increasing experimental evidence suggests that soluble oligomers formed early during the aggregation process are the most cytotoxic forms of αS. This study investigated the uptake by neuronal cells of pathologically relevant αS oligomers and fibrils exploiting a range of conformation-sensitive antibodies, and the super-resolution stimulated emission depletion (STED) microscopy. We found that prefibrillar oligomers promptly penetrate neuronal membranes, thus resulting in cell dysfunction. By contrast, fibril docking to the phospholipid bilayer is accompanied by αS conformational changes with a progressive release of A11-reactive oligomers, which can enter into the neurons and trigger cell impairment. Our data provide important evidence on the role of αS fibrils as a source of harmful oligomers, which resemble the intermediate conformers formed de novo during aggregation, underling the dynamic and reversible nature of protein aggregates responsible for α-synucleinopathies.

Constructing threat through quotes and historical analogies in the Czech and the US “Ukraine Discourse”

2019 ◽  
Vol 18 (6) ◽  
pp. 870-892
Author(s):  
Martina Berrocal
Keyword(s):  
Emotional Response ◽  
Political Discourse ◽  
Theoretical Framework ◽  
Temporal Dimension ◽  
Parliamentary Debates ◽  
Intended Action ◽  
The Us ◽  
Discursive Representations ◽  
The Way

Abstract Conflicts and their discursive representations involve, apart from the spacio-temporal dimension, also the socio-ideological and axiological positions. These prompt the desired emotional response from the audience in a form of authorization for the intended action. All these dimensions are mainly construed by presenting series of assertions by creating the dichotomy self-other and by triggering implicatures that contribute to the preferred interpretations of the presented representations. This paper aims to examine the role of quotes and historical analogies triggered by quotes in discourse, concretely, it focuses on the way the Ukrainian conflict is proximized in the US and the Czech political discourse, namely in the parliamentary debates and governmental statements (November 2013–December 2014). The theoretical framework applied is the proximization approach (Cap 2008, 2010, 2013, 2014, 2017) which is complemented by the studies that explore the pragmatic functions of quoting in discourse.

scholarly journals Crafting Emotions: The valence of time in narratives about the future of Europe in the Council of Europe (1949)

2021 ◽  
Vol 17 (4) ◽  
Author(s):  
Martijn Kool ◽  
Trineke Palm
Keyword(s):  
European Integration ◽  
Narrative Approach ◽  
Council Of Europe ◽  
Temporal Dimension ◽  
Historical Approach ◽  
Policy Ideas ◽  
The Future ◽  
Set Up

How are emotional narratives used to mobilise support for or opposition against policy ideas about the institutional set-up of European integration? This article systematically examines the first General Debate of the Consultative Assembly of the Council of Europe in 1949, which featured as a laboratory for the rise and demise of various blueprints for European integration. This article makes a threefold contribution. First, it introduces a narrative approach that combines the valence of emotions with their temporal dimension. Second, it demonstrates how these emotionally charged narratives of hope, redemption, fear and sacrifice provide the affective glue of an emerging (transnational) emotional community that cuts through nationality and political colour. Third, taking a historical approach this article points at the need to historicise the role of emotions in European integration.

Sign in / Sign up

Export Citation Format

Share Document