Fibre-Optic and Nanoparticle-Based Fluorescence Sensing Using Indicator Dyes: Pitfalls, Self- Referencing, Application, and Future Trends

2008 ◽  
pp. 347-372 ◽  
Author(s):  
Gerhard J. Mohr
Keyword(s):  
Future Trends ◽  
Fibre Optic ◽  
Fluorescence Sensing ◽  
Indicator Dyes

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

On the origin of the rising incidence of esophageal adenocarcinoma and prediction of future trends

2001 ◽  
Vol 120 (5) ◽  
pp. A30-A30
Author(s):  
J HELM ◽  
J BARTHEL ◽  
D COPPOLA ◽  
P LAZARUS ◽  
S LUTHER ◽  
...  
Keyword(s):  
Esophageal Adenocarcinoma ◽  
Future Trends ◽  
Rising Incidence

Families in Society: Current Research and Future Trends

1996 ◽  
Vol 41 (5) ◽  
pp. 466-467
Author(s):  
Lori A. Ingram
Keyword(s):  
Future Trends

Fibre-optic temperature sensor with wide temperature range characteristics

1987 ◽  
Vol 134 (5) ◽  
pp. 291 ◽  
Author(s):  
K.T.V. Grattan ◽  
J.D. Manwell ◽  
S.M.L. Sim ◽  
C.A. Willson
Keyword(s):  
Wide Temperature Range ◽  
Temperature Sensor ◽  
Fibre Optic ◽  
Temperature Range

Future trends in health monitoring of materials

2001 ◽  
Vol 89 ◽  
pp. 31-33
Author(s):  
L. Flandin ◽  
Y. Bréchet ◽  
J.Y. Cavaillé
Keyword(s):  
Health Monitoring ◽  
Future Trends
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