Fluorescence Sensing and Imaging Using Pressure-Sensitive Paints and Temperature-Sensitive Paints

Author(s):  
Matthias I. J. Stich ◽  
Otto S. Wolfbeis
1997 ◽  
Author(s):  
M. Woodmansee ◽  
J. Dutton ◽  
M. Woodmansee ◽  
J. Dutton

1999 ◽  
Vol 65 (4) ◽  
pp. 1710-1720 ◽  
Author(s):  
Eric E. Allen ◽  
Daniel Facciotti ◽  
Douglas H. Bartlett

ABSTRACT There is considerable evidence correlating the production of increased proportions of membrane unsaturated fatty acids (UFAs) with bacterial growth at low temperatures or high pressures. In order to assess the importance of UFAs to microbial growth under these conditions, the effects of conditions altering UFA levels in the psychrotolerant piezophilic deep-sea bacterium Photobacterium profundum SS9 were investigated. The fatty acids produced byP. profundum SS9 grown at various temperatures and pressures were characterized, and differences in fatty acid composition as a function of phase growth, and between inner and outer membranes, were noted. P. profundum SS9 was found to exhibit enhanced proportions of both monounsaturated (MUFAs) and polyunsaturated (PUFAs) fatty acids when grown at a decreased temperature or elevated pressure. Treatment of cells with cerulenin inhibited MUFA but not PUFA synthesis and led to a decreased growth rate and yield at low temperature and high pressure. In addition, oleic acid-auxotrophic mutants were isolated. One of these mutants, strain EA3, was deficient in the production of MUFAs and was both low-temperature sensitive and high-pressure sensitive in the absence of exogenous 18:1 fatty acid. Another mutant, strain EA2, produced little MUFA but elevated levels of the PUFA species eicosapentaenoic acid (EPA; 20:5n-3). This mutant grew slowly but was not low-temperature sensitive or high-pressure sensitive. Finally, reverse genetics was employed to construct a mutant unable to produce EPA. This mutant, strain EA10, was also not low-temperature sensitive or high-pressure sensitive. The significance of these results to the understanding of the role of UFAs in growth under low-temperature or high-pressure conditions is discussed.


2000 ◽  
Vol 123 (4) ◽  
pp. 823-829 ◽  
Author(s):  
Kelly R. Navarra ◽  
Douglas C. Rabe ◽  
Sergey D. Fonov ◽  
Larry P. Goss ◽  
Chunill Hah

An innovative pressure-measurement technique that employs the tools of molecular spectroscopy has been widely investigated by the aerospace community. Measurements are made via oxygen-sensitive molecules attached to the surface of interest as a coating, or paint. The pressure-sensitive-paint (PSP) technique is now commonly used in stationary wind-tunnel tests; this paper presents the use of this technique in advanced turbomachinery applications. New pressure- and temperature-sensitive paints (P/TSPs) have been developed for application to a state-of-the-art transonic compressor where pressures up to 1.4 atm and surface temperatures to 90°C are expected for the suction surface of the first-stage rotor. PSP and TSP data images have been acquired from the suction surface of the first-stage rotor at 85 percent of the corrected design speed for the compressor near-stall condition. A comparison of experimental results with CFD calculations is discussed.


Author(s):  
Kil-Ju Moon ◽  
Hideo Mori ◽  
Yuichiro Ambe ◽  
Hiroaki Kawabata

Pressure sensitive paint (PSP) and temperature sensitive paint (TSP) are useful measurement tools in measurement of pressure and temperature distribution on surfaces in airflows, and application of PSP and TSP in relatively low speed flow fields is highly demanded. However, temperature dependence of PSP will be the factor of an error in the pressure measurement, and therefore the temperature compensation is needed to obtain highly precise pressure distribution. To solve this problem, we apply new combining method. This is made by stacking up a PSP layer and a TSP layer. PSP emission and TSP emission can be separated each other using optical filters. Using this combining method, temperature distribution obtained by the TSP can be used for the temperature compensation of the PSP. In this research we call this combining method as “dual-layer PSP/TSP”. We clarify that the sensitivity of the dual-layer PSP/TSP in low-speed flow fields against pressure and temperature is comparable to that of mono-layer PSP or TSP, showing the feasibility of the dual-layer PSP/TSP for the measurement in low-speed flow fields with the gauge pressure below 1kPa and temperature in the range of 10–20°C (283–293K). We also clarify that, using dual-layer PSP/TSP method, the accuracy of pressure data obtained by PSP increases by compensation of temperature which is obtained by TSP.


Author(s):  
Tomohiro Kameya ◽  
Yu Matsuda ◽  
Hiroki Yamaguchi ◽  
Yasuhiro Egami ◽  
Tomohide Niimi

There appears fluttering phenomena in a hard disk drive system with high-speed disks rotating inside a closed space, leading to degrade of reading and writing performance. The precise pressure distribution on the disk may improve the performance, but there has been no report because it is very hard to measure the surface pressure using conventional techniques, such as pressure taps. While pressure sensitive paint (PSP) seems to be suitable for the pressure measurement on the disk, we have to compensate its highly temperature-sensitive characteristics of PSP, because the temperature distribution on the disk is not assumed to be uniform. We employed PySO3H based PSP, which has small temperature sensitivity, and have obtained the pressure distribution on the disk rotated at various speeds (10000–20000 rpm) successfully. The result showed that the pressure is higher at the disk outside than at the center, and forms a concentric circle distribution. Moreover, we found that the pressure difference between the inner and outer region of the disk increases as a square of disk rotation speed.


Author(s):  
Kelly R. Navarra ◽  
Douglas C. Rabe ◽  
Larry P. Goss

An innovative pressure-measurement technique that employs the tools of molecular spectroscopy has been widely investigated by the aerospace community. Measurements are made via oxygen-sensitive molecules attached to the surface of interest as a coating, or paint. The pressure-sensitive-paint (PSP) technique is now commonly used in stationary wind-tunnel tests; this paper presents the use of this technique in advanced turbomachinery applications. New pressure- and temperature-sensitive paints (P/TSPs) have been developed for application to a state-of-the-art transonic compressor where pressures up to 1.4 atm and surface temperatures to 90°C are expected for the suction surface of the first-stage rotor. PSP and TSP data images have been acquired from the suction surface of the first-stage rotor at 85% of the corrected design speed for the compressor near-stall condition. A comparison to over-the-rotor pressure transducer measurements will be presented.


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