Neurotensin and Neuromedin N Are Differentially Processed from a Common Precursor by Prohormone Convertases in Tissues and Cell Lines

2009 ◽  
pp. 63-84 ◽  
Author(s):  
Patrick Kitabgi
Keyword(s):  
Cell Lines ◽  
Prohormone Convertases ◽  
Common Precursor ◽  
Neuromedin N

Proneurotensin/neuromedin N secreted from small cell lung carcinoma cell lines as a potential tumor marker

2008 ◽  
Vol 2 (12) ◽  
pp. 1620-1627 ◽  
Author(s):  
Shun-ichiro Ogura ◽  
Kumi Kaneko ◽  
Shoji Miyajima ◽  
Keiichi Ohshima ◽  
Ken Yamaguchi ◽  
...  
Keyword(s):  
Tumor Marker ◽  
Cell Lines ◽  
Lung Carcinoma ◽  
Carcinoma Cell ◽  
Small Cell Lung Carcinoma ◽  
Small Cell ◽  
Small Cell Lung ◽  
Cell Lung Carcinoma ◽  
Carcinoma Cell Lines ◽  
Neuromedin N

Immunological detection of prohormone convertases in two different proglucagon processing cell lines

FEBS Letters ◽  
1994 ◽  
Vol 344 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Philippe Blache ◽  
Dung Le-Nguyen ◽  
Catherine Boegner-Lemoine ◽  
Anne Cohen-Solal ◽  
Dominique Bataille ◽  
...  
Keyword(s):  
Cell Lines ◽  
Prohormone Convertases ◽  
Immunological Detection

Pro-neurotensin/Neuromedin N Expression and Processing in Human Colon Cancer Cell Lines

1998 ◽  
Vol 246 (1) ◽  
pp. 155-159 ◽  
Author(s):  
Carole Rovère ◽  
Pierre Barbero ◽  
Jean-José Maoret ◽  
Marc Laburthe ◽  
Patrick Kitabgi
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines ◽  
Neuromedin N

Marked variations of the relative distributions of neurotensin and neuromedin N in micropunched rat brain areas suggest differential processing of their common precursor

1991 ◽  
Vol 124 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Patrick Kitabgi ◽  
Yoshinori Masuo ◽  
Arnaud Nicot ◽  
Anne Berod ◽  
Jean-Claude Cuber ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Areas ◽  
Differential Processing ◽  
Common Precursor ◽  
Neuromedin N

DNA methylation contributes to expression of the human neurotensin/neuromedin N gene

1998 ◽  
Vol 274 (3) ◽  
pp. G535-G543 ◽  
Author(s):  
Zizheng Dong ◽  
Xiaofu Wang ◽  
Qingzheng Zhao ◽  
Courtney M. Townsend ◽  
B. Mark Evers
Keyword(s):  
Dna Methylation ◽  
Cell Lines ◽  
N Gene ◽  
Hepatoma Cell Line ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Gut Hormone ◽  
Liver Cancers ◽  
G2 Cells ◽  
Neuromedin N

The gut and liver share a common embryological origin. The gene encoding the gut hormone neurotensin/neuromedin N (NT/N) is expressed in the adult small bowel, and NT/N is transiently expressed in the fetal liver, suppressed in the adult liver, and reexpressed in certain liver cancers. In our present study, we found that the NT/N gene was expressed at high levels in the human hepatoma cell line Hep 3B but was not expressed in Hep G2 cells. To further determine the mechanisms regulating NT/N expression, we performed Southern blotting and gene cloning techniques. Neither alteration nor mutation of the NT/N gene was responsible for this differential NT/N expression pattern. Human NT/N promoter constructs were transfected into either Hep 3B or Hep G2. Both cell lines supported NT/N transcription, indicating that the absence of NT/N expression in Hep G2 cells was due to mechanisms other than the absence of positive transcription factors. The role of DNA methylation was next assessed. Methylation of NT/N promoter constructs in vitro resulted in a 67-fold reduction in promoter activity, whereas treatment with the demethylating agent 5-azacytidine induced NT/N expression in Hep G2 cells, thus suggesting that DNA methylation plays a role in the expression of the gut endocrine gene NT/N. Defining the mechanisms regulating NT/N expression in these hepatic-derived cell lines will provide not only a better understanding of cell-specific and developmental regulation of a gut endocrine gene but also possible insight into liver cell lineage patterns and the derivation of certain hepatocellular cancers.

(EB) Virus: Latency and Expression in Transplanted and Cultured Human Cells

1971 ◽  
Vol 29 ◽  
pp. 368-369
Author(s):  
B. G. Uzman ◽  
M. M. Kasac ◽  
H. Saito ◽  
A. Krishan
Keyword(s):  
Cell Lines ◽  
Primary Cultures ◽  
Virus Particles ◽  
Barr Virus ◽  
Virus Latency ◽  
Virus Surveillance ◽  
Cultured Human Cells ◽  
Epstein Barr ◽  
Serial Transplantation ◽  
Tubular Arrays

In conjunction with the cultivation and transplantation of cells from human tumors by the Programs of Microbiology and Immunogenetics, virus surveillance by electron microscopy has been routinely employed. Of particular interest in this regard have been 3 cell lines cultured from lymph nodes or spleen of 2 patients with Hodgkin's disease and 1 patient with Letterer-Siwe's disease. Each of these cell lines when transplanted in Syrian hamster neonates conditioned with anti-lymphocyte serum grew as serially transplantable tumors; from such transplants of the 3 cell lines cell cultures were retrieved.Herpes type virus particles (Figs. 1, 2, 3) were found in the primary cultures of all three lines, in frozen thawed aliquots of same, and in cultures retrieved from their tumors growing by serial transplantation in hamsters. No virus was detected in sections of 25 of the serially transplanted tumors. However, in 10 such tumors there were repeated instances of tubular arrays in the cisternae of the endoplasmic reticulum (Fig. 4). On serologic examination the herpes virus was shown to be the Epstein-Barr virus.

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