Light-scattering in Turbid Fluids: Scattering Intensity and Amplitude of the Auto-correlation Function

Author(s):  
J. Köser ◽  
F. Kuhnen ◽  
D. Saracsan ◽  
W. Schröer
2019 ◽  
Author(s):  
Dmitry D. Postnov ◽  
Jianbo Tang ◽  
Sefik Evren Erdener ◽  
Kıvılcım Kılıç ◽  
David A. Boas

ABSTRACTUtilizing a high-speed camera and recording back-scattered laser light at more than 20,000 frames per second, we introduce the first wide-field dynamic laser speckle imaging (DLSI) in which we are able to quantify the laser speckleintensity temporal auto-correlation function g2(τ) for every pixel individually to obtain a quantitative image of the dynamics of the light scattering particles in the sample. The ability to directly and quantitatively measure the intensity auto-correlation function allows us to solve the problem of how to quantitatively interpret data measured by laser speckle contrast imaging (LSCI), multi-exposure laser speckle imaging (MESI) and laser Doppler flowmetry (LDF). The intensity auto-correlation function is related to the field temporal auto-correlation function g1(τ), which has been quantitatively related to the dynamics of the light scattering particles including flowing red blood cells. The form of g1(τ) depends on the amount of light scattering (i.e. single or multiple scattering) and the type of particle motion (i.e. ordered or unordered). Although these forms of the field correlation functions have been established for over 30 years, there is no agreement nor experimental support on what scattering and motion regimes are relevant for the varied biomedical applications. We thus apply DLSI to image cerebral blood flow in mouse through a cranial window and show that the generally accepted form of g1(τ), is applicable only to visible surface vessels of a specific size (20 – 200μm). We demonstrate that for flow in smaller vessels and in parenchymal regions that the proper g1(τ) form corresponds with multiple scattering light and unordered motion which was never considered to be relevant for these techniques. We show that the wrong assumption for the field auto-correlation model results in a severe underestimation of flow changes when measuring blood flow changes during ischemic stroke. Finally, we describe how DLSI can be integrated with other laser speckle methods to guide model selection, or how it can be used by itself as a quantitative blood flow imaging technique.


1990 ◽  
Vol 55 (4) ◽  
pp. 1022-1032 ◽  
Author(s):  
Čestmír Koňák ◽  
Jaromír Jakeš ◽  
František Petráš ◽  
Marie Kárská ◽  
Jan Peřina

The effect of multiple light scattering on a homodyne intensity auto-correlation function and photocount distribution of scattered light has been studied. The analysis of quasielastic light scattering data has shown that even a small contribution (several percent) of multiple light scattering to the total scattered light intensity can be distinguished and identified by the Laplace transform inversion of the corresponding intensity auto-correlation function. The photocount distribution reflects the coherence time changes of the scattered light only.


2015 ◽  
Vol 14 (1) ◽  
Author(s):  
I Nyoman Pramaita ◽  
I G.A.G.K. Diafari ◽  
DNKP Negara ◽  
Agus Dharma

In this paper, the authors propose the design of a new orthogonal small set Kasami code sequence generated using combination of non-orthogonal m-sequence and small set Kasami code sequence. The authors demonstrate that the proposed code sequence has comparable auto-correlation function (ACF), cross- correlation function (CCF), peak cross-correlation values with that of the existing orthogonal small set Kasami code sequence. Though the proposed code sequence has less code sequence sets than that of the existing orthogonal small set Kasami code sequence, the proposed code sequence possesses one more numbers of members in each code sequence set. The members of the same code set of the proposed code sequence are orthogonal to each other.


Author(s):  
Tilo Schwalger

AbstractNoise in spiking neurons is commonly modeled by a noisy input current or by generating output spikes stochastically with a voltage-dependent hazard rate (“escape noise”). While input noise lends itself to modeling biophysical noise processes, the phenomenological escape noise is mathematically more tractable. Using the level-crossing theory for differentiable Gaussian processes, we derive an approximate mapping between colored input noise and escape noise in leaky integrate-and-fire neurons. This mapping requires the first-passage-time (FPT) density of an overdamped Brownian particle driven by colored noise with respect to an arbitrarily moving boundary. Starting from the Wiener–Rice series for the FPT density, we apply the second-order decoupling approximation of Stratonovich to the case of moving boundaries and derive a simplified hazard-rate representation that is local in time and numerically efficient. This simplification requires the calculation of the non-stationary auto-correlation function of the level-crossing process: For exponentially correlated input noise (Ornstein–Uhlenbeck process), we obtain an exact formula for the zero-lag auto-correlation as a function of noise parameters, mean membrane potential and its speed, as well as an exponential approximation of the full auto-correlation function. The theory well predicts the FPT and interspike interval densities as well as the population activities obtained from simulations with colored input noise and time-dependent stimulus or boundary. The agreement with simulations is strongly enhanced across the sub- and suprathreshold firing regime compared to a first-order decoupling approximation that neglects correlations between level crossings. The second-order approximation also improves upon a previously proposed theory in the subthreshold regime. Depending on a simplicity-accuracy trade-off, all considered approximations represent useful mappings from colored input noise to escape noise, enabling progress in the theory of neuronal population dynamics.


Microscopy ◽  
2019 ◽  
Vol 68 (5) ◽  
pp. 395-412
Author(s):  
Shigeto Isakozawa ◽  
Misuzu Baba ◽  
Junpei Amano ◽  
Shohei Sakamoto ◽  
Norio Baba

Abstract The spot auto-focusing (AF) method with a unique high-definition auto-correlation function (HD-ACF) proposed in the previous paper is improved and is now applicable to general specimens at a wide range of magnifications. According to the definition where the AF is defocused to obtain the highest resolution, the proposed method achieves the sharpest HD-ACF profile in the AF spot image. The relationship where the sharpest HD-ACF profile gives the highest resolution is theoretically explained, and practical AF examples for different specimens and magnifications are experimentally demonstrated. Specimens include a yeast cell thin section at 10-k magnification, a standard grating replica used as a ruler at 50-k, a crystal lattice of graphitized carbon at 400-k and a 60°-tilted thin section (yeast cell) at 10-k. Different procedures are prepared to actively identify the defocus position that gives the sharpest HD-ACF profile. Every AF result demonstrates the highest-resolution image.


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