Highly Aligned Polymer Nanofiber Structures: Fabrication and Applications in Tissue Engineering

2011 ◽  
pp. 171-212 ◽  
Author(s):  
Vince Beachley ◽  
Eleni Katsanevakis ◽  
Ning Zhang ◽  
Xuejun Wen
Keyword(s):  
Tissue Engineering ◽  
Polymer Nanofiber

Electrospinning 3D Scaffolds for use in Neural Tissue Engineering

2015 ◽  
Vol 1798 ◽  
Author(s):  
Rachel Martin ◽  
M. E. Mullins ◽  
F. Zhao ◽  
Zichen Qian
Keyword(s):  
Tissue Engineering ◽  
3D Structure ◽  
Neural Tissue ◽  
Axonal Growth ◽  
Growth Media ◽  
Neural Tissue Engineering ◽  
Growth Environment ◽  
Polymer Nanofiber

ABSTRACTPolymer nanofiber scaffolds for use in neural tissue engineering have been fabricated via electrospinning of poly-L-lactic acid (PLLA) directly onto a 3D printed support. Previously, the investigators have shown success in promoting the directed growth of neural axons on highly aligned PLLA substrates both in vitro and in vivo. However, one criticism of the earlier in vitro studies is that by spinning fibers on a flat, two-dimensional surface, the growth of the axons is restricted to one plane. Thus the axon-to-fiber attachment may not be the sole mechanism for aligning the growth of the axons along the fibers, and the channels between the fibers and the substrate could contribute to the results. Using 3D-printing, elevated or “bridge” spinning stages were made with supports at varying heights, allowing the fibers to be suspended 2 to 5 mm above the substrate surface in different configurations. This 3D structure promotes better access of in vitro cell cultures on the fibers to the growth media during incubation, reduces substrate effects, allows more degrees of freedom for axonal growth, and more closely simulates the growth environment found in vivo. Using these 3D stages, we have electrospun free-standing, highly-aligned pure PLLA fiber scaffolds. We are exploring spinning coaxial fibers with a PLLA sheath and a second core polymer. These coaxial fiber scaffold structures offer additional opportunities for in situ delivery of growth agents and/or electrical stimulation for improved axonal growth results.

scholarly journals Bare laser-synthesized plasmonic Au and TiN nanoparticles as functional additives to polymer nanofiber platforms for tissue engineering applications

2021 ◽  
Vol 2058 (1) ◽  
pp. 012002
Author(s):  
A Al-Kattan ◽  
A V Kabashin
Keyword(s):  
Tissue Engineering ◽  
Near Infrared ◽  
Laser Ablation In Liquid ◽  
Optical Biosensing ◽  
Functional Additives ◽  
Tin Nanoparticles ◽  
Polymer Nanofiber ◽  
Ligand Free ◽  
Synthesis Routes ◽  
Ultrashort Laser

Abstract Exhibiting strong optical absorption in the visible – near-infrared, plasmonic nanomaterials can be used as transducers in optical biosensing, contrast agents in bioimaging and synthesizers of photothermal therapy. Such functionalities promise their employment as functional elements in tissue engineering platforms, but such applications typically require ultraclean nanomaterials to minimize toxicity problems, which is not easy using conventional chemical synthesis routes. We recently demonstrated the possibility of fabricating ultraclean bare (ligand-free) plasmonic Au and TiN nanoparticles by ultrashort laser ablation in liquid ambient. Exempt of any toxic contaminants and exhibiting a series of imaging and therapeutic functionalities, these nanomaterials present promising objects for various biomedical applications. Here, we review our recent progress in the co-electrospinning of laser-synthesized Au and TiN nanoparticles with polymers to form functionalized matrices for tissue engineering.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Combination of MRI and 99mTc-NTP 15-5 Scintigraphy for the Assessment of Intervertebral Disk Degeneration and Tissue Engineering

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1319873-s-0032-1319873
Author(s):  
P. Colombier ◽  
J. Clouet ◽  
E. Miot-Noirault ◽  
A. Vidal ◽  
F. Cachin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Intervertebral Disk ◽  
Intervertebral Disk Degeneration ◽  
Disk Degeneration
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