IR Spectroscopic Techniques to Study Isolated Biomolecules

2014 ◽  
pp. 1-42 ◽  
Author(s):  
Anouk M. Rijs ◽  
Jos Oomens
Keyword(s):  
Spectroscopic Techniques ◽  
Ir Spectroscopic

scholarly journals Synthesis characterization and antibacterial studies of 4-aminoantipyrine schiff’s bases

2017 ◽  
Vol 2 (01) ◽  
pp. 8-14 ◽  
Author(s):  
K Sunand ◽  
K Vinay Kumar ◽  
K Ashwini ◽  
P Suresh Kumar ◽  
S Vishnu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Schiff Bases ◽  
Vital Role ◽  
Spectroscopic Techniques ◽  
Schiff’S Bases ◽  
Pharmacological Activities ◽  
Schiff's Bases ◽  
Ir Spectroscopic

Aim: To synthesize and evaluate 4-aminoantipyrine related schiff’s bases as antibacterial agents. Objective: To synthesize, purify, characterize and evaluate 4-aminoantipyrine. Method: Schiff bases derived from 4-aminoantipyrine play a vital role in biological and pharmacological activities. Knowing the importance of 4-aminoatipyrine schiff bases and their analogues wide varieties of bioactivities like analgesic, antiviral, antipyretic, anti-rheumatic, antimicrobial and anti-inflammatory activities have been widely studied. 4-aminoantipyrine compounds C1 (anisaldehyde), C2 (p-hydroxybenzaldehyde) and C3(vanillin) were prepared by condensation between 4-amino antipyrine and substituted aromatic benzaldehydes. The products were purified by recrystallization by using ethanol, characterized by IR spectroscopy. The N-H stretching in 4-aminoantipyrine is shown at 3430 cm-1 and -3325 cm-1. The -HC=N- stretching is observed in the range of 1508-1504 cm-1 The –OCH3 stretching is found at 1888 cm-1. 4-amino antipyrine related schiff’s bases evaluated their activity as antimicrobials in-vitro by spread plate method against E.coli. Schiff bases have potent antibacterial activity with gram negative bacteria E.coli. Results: Synthesis and characterization of a schiff bases derived from substituted benzaldehydes and 4-aminoantipyrine was evaluated and characterized with the IR spectroscopic techniques and schiff bases have shown potent antibacterial activity against E.Coli.

Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial

2021 ◽  
pp. 5297-5304
Author(s):  
Rini Hamsidi ◽  
Wahyuni Wahyuni ◽  
Adryan Fristiohady ◽  
Muhammad Hajrul Malaka ◽  
Idin Sahidin ◽  
...  
Keyword(s):  
Antimalarial Activity ◽  
Carthamus Tinctorius ◽  
Spectroscopic Techniques ◽  
1H And 13C Nmr ◽  
Marker Compound ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Spectroscopic

Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in the production of antimalarial drugs. The purpose of this study was to isolate and identify the steroid compounds in the safflower and determine its antimalarial activity in vitro. The isolation process was conducted through extraction and chromatography methods. Then, the characterization of the isolated compounds was conducted through spectroscopic techniques which include Fourier Transform Infrared Spectroscopy (FT-IR), NMR 1-D (1H and 13C-NMR), and NMR 2-D (HMQC, HMBC, and H-H COZY) as well as comparing data with the existing literatures. In addition, the tests conducted were with variations of isolate concentrations (10, 1, 0.1, 0.01, and 0.001 μg/mL) against 3D7 strain of Plasmodium falciparum. Based on the FT-IR spectroscopic data, the steroid compounds isolated from safflowers might be stigmasterols. In addition, the isolates had -OH functional group in the region of 3431 cm-1, C-O in the region of 1053 cm-1, and Csp3-H in regions of 2960, 2934, and 2865 cm-1. The NMR 1-D data showed presence of 29 carbon atoms, while the protons were 48 in number. Furthermore, the IC50 value of the compound was 34.03 μg/mL with a percentage inhibition of 43.92% against the growth of P. falciparum. Therefore, it was classified as inactive agent in inhibiting the growth of malaria parasites, however, it could be used as a marker compound in C. tinctorius Linn extract.

scholarly journals Conversion of fisheries waste as magnetic hydroxyapatite bionanocomposite for the removal of heavy metals from groundwater

2017 ◽  
Vol 18 (4) ◽  
pp. 1406-1419
Author(s):  
F. Elmi ◽  
R. Chenarian Nakhaei ◽  
H. Alinezhad
Keyword(s):  
Heavy Metals ◽  
Freundlich Isotherm ◽  
Spectroscopic Techniques ◽  
Order Kinetic ◽  
Fish Scale ◽  
X Ray Diffraction ◽  
X Ray ◽  
Removal Of Heavy Metals ◽  
Order Kinetic Model ◽  
Ir Spectroscopic

Abstract This study is the first report of its type demonstrating the synthesis of mHAP on the basis of magnetic functionalization with nHAP, which were synthesized using Rutilus frisii kutum fish scale as a benign fishery waste by-product. The mHAP was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray diffraction (EDX), and Fourier transform infrared (FT-IR) spectroscopic techniques. The XRD pattern confirmed the formation of a single-phase nHAP without any extra steady phases. It was also found that the pseudo-second-order kinetic model gave a satisfactory fit to the experimental data (R2 = 0.99). The maximum removal percentages of Cu and Zn ions in optimal conditions (adsorbent dosage at 0.1 g, 30 min contact time at 25 ± 1 °C and pH = 5 ± 0.1) by mHAP were 97.1% and 93.8%, respectively. Results also demonstrated that mHAP could be recycled for up to five cycles in the case of copper and zinc. The Langmuir isotherm was proved to have a better correlation compared with that of the Freundlich isotherm. The thermodynamic parameters indicated that it was a spontaneously endothermic reaction. In conclusion, mHAP could be regarded as a powerful candidate for efficient biosorbent, capable of adsorbing heavy metals from aqueous solutions.

scholarly journals Crystal Structure and Antitumor Activity of the Novel Zwitterionic Complex of tri-n-Butyltin(IV) with 2-Thiobarbituric Acid

2008 ◽  
Vol 2008 ◽  
pp. 1-5 ◽  
Author(s):  
Vasilios I. Balas ◽  
Sotiris K. Hadjikakou ◽  
Nick Hadjiliadis ◽  
Nikolaos Kourkoumelis ◽  
Mark E. Light ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Thiobarbituric Acid ◽  
Wistar Rat ◽  
In Vitro Cytotoxicity ◽  
Spectroscopic Techniques ◽  
X Ray Diffraction ◽  
Polycyclic Aromatic ◽  
Zwitterionic Complex ◽  
Ir Spectroscopic

A novel tri-n-butyl(IV) derivative of 2-thiobarbituric acid (HTBA) of formula[(n-Bu)3Sn(TBA)⋅H2O](1) has been synthesized and characterized by elemental analysis and119Sn-NMR and FT-IR spectroscopic techniques. The crystal structure of complex1has been determined by single crystal X-ray diffraction analysis at 120(2) K. The geometry around Sn(IV) is trigonal bipyramidal. Threen-butyl groups and one oxygen atom from a deprotonated 2-thiobarbituric ligand are bonded to the metal center. The geometry is completed with one oxygen from a water molecule. Compound1exhibits potent, in vitro, cytotoxicity against sarcoma cancer cells (mesenchymal tissue) from the Wistar rat, polycyclic aromatic hydrocarbons (PAH, benzo[a]pyrene) carcinogenesis. In addition, the inhibition caused by1, in the rate of lipoxygenase (LOX) catalyzed oxidation reaction of linoleic acid to hyperoxolinoleic acid, has been also kinetically and theoretically studied. The results are compared to that of cisplatin.

Initial stages of propane activation over Zn/MFI catalyst studied by in situ NMR and IR spectroscopic techniques

2006 ◽  
Vol 238 (1) ◽  
pp. 122-133 ◽  
Author(s):  
Y KOLYAGIN ◽  
V ORDOMSKY ◽  
Y KHIMYAK ◽  
A REBROV ◽  
F FAJULA ◽  
...  
Keyword(s):  
Spectroscopic Techniques ◽  
Propane Activation ◽  
Ir Spectroscopic ◽  
In Situ Nmr

scholarly journals Synthesis of substituted pyridine based sulphonamides as an antidiabetic agent

2021 ◽  
Vol 12 (3) ◽  
pp. 279-283
Author(s):  
Gautam Sadawarte ◽  
Samadhan Jagatap ◽  
Mukesh Patil ◽  
Vasant Jagrut ◽  
Jamatsing Darbarsing Rajput
Keyword(s):  
Heterocyclic Compounds ◽  
Biological Activities ◽  
Research Work ◽  
Synthetic Methods ◽  
Alpha Amylase ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Antidiabetic Agent ◽  
Biological Functions ◽  
Ir Spectroscopic

This research work describes the synthesis of a new series of heterocyclic compounds, namely sulfonamide derivatives. Sulfonamides are a diverse class of organic compounds having significant and potent biological activities. Diverse synthetic methods have been engaged to build up its various derivatives for different biological functions. In this study, the production of novel pyridine-based heterocyclic compounds having sulfonamide moieties has been elaborated. The obtained sulfonamide-based pyridine scaffold was used to investigate their alpha-amylase inhibition activity. The structures of freshly prepared compounds were described using 1H NMR, 13C NMR, and IR spectroscopic techniques. The molecular docking of sulfonamides performed against porcine pancreatic alpha-amylase using PDB file 1LP was used for generation of grid. All the new synthesized compounds were shown notable anti-diabetic activity.

Photocatalytic Degradation of Methylene Blue Dye over Copper(II) Complex Nanoparticle Catalyst.

2021 ◽  
pp. 138-142
Author(s):  
Hassan Wafi Garba ◽  
Abubakar Garba Ashiru ◽  
Rooshan Watanpal ◽  
Mohammed Bello ◽  
Kasimu Abubakar ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Single Phase ◽  
Uv Light ◽  
Blue Dye ◽  
Spectroscopic Techniques ◽  
Methylene Blue Dye ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Physicochemical And Structural Properties ◽  
Nanoparticle Catalyst

Abstract—A novel copper(II) complex nanoparticles catalyst was synthesized via precipitation and calcination. The catalyst was applied for the degradation of methylene blue under UV light irradiation. The catalyst was characterized for its physicochemical and structural properties by XRD, SEM, TEM and FT-IR spectroscopic techniques. XRD studies revealed that the particles were monoclinic single phase crystalline structure, the morphology of the nanostructure was confirmed by SEM while the TEM studies revealed that the particles were FCC. FTIR spectra showed the presence of diverse vibrational functional groups. Photolysis of the methylene blue dye indicates no degradation after 1 hour reaction, while the addition of the copper(II) complex nanoparticles catalyst resulted in the decolouration of the dye by ~94%. The efficiency of the catalyst was attributed to the nanoparticle’s morphology.

Structural Identification of Products from the Chloromethylation of Salicylaldehyde

Synlett ◽  
2018 ◽  
Vol 30 (01) ◽  
pp. 44-48 ◽  
Author(s):  
Muhammad Bala ◽  
Ebrahim Kadwa ◽  
Holger Friedrich
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
High Resolution Mass Spectrometry ◽  
Structural Identification ◽  
Spectroscopic Techniques ◽  
X Ray Diffraction ◽  
X Ray ◽  
Multinuclear Nmr Spectroscopy ◽  
Ir Spectroscopic ◽  
Resolution Mass

In the functionalization of salicylaldehyde to give 5-(chloromethyl)salicylaldehyde, two byproducts [5-(hydroxymethyl)salicylaldehyde and 5,5′-methylenebis(salicylaldehyde)] were also isolated. ­Detailed characterizations and structural analyses of all three products by single-crystal X-ray diffraction, multinuclear NMR spectroscopy, high-resolution mass spectrometry, and IR spectroscopic techniques are presented and discussed. A strategy is presented for the preferential isolation of the two byproducts through column chromatography.

scholarly journals Synthesis of Selective Bioactive Pyridylpyridones: in silico Studies and Biological Evaluations

2020 ◽  
Vol 32 (5) ◽  
pp. 985-994
Author(s):  
Suresh Ramasamy ◽  
Singanan Ponnuchamy ◽  
Sivakumar Ponnusamy
Keyword(s):  
Molecular Docking ◽  
Molecular Electrostatic Potential ◽  
Docking Studies ◽  
Spectroscopic Techniques ◽  
Biological Applications ◽  
In Silico Studies ◽  
Potential Mapping ◽  
Ft Ir ◽  
Additional Support ◽  
Ir Spectroscopic

Twenty three substituted pyridylpyridones were designed and performed for molecular docking studies against α-amylase enzyme. The top three hit molecules were synthesized and characterized by 1H NMR, 13C NMR, ESI-mass and FT-IR spectroscopic techniques. Experimental biological applications were studied for these compounds. The DFT calculations were executed for the hit compounds. In addition, molecular electrostatic potential mapping was also executed for additional support.

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