Investigation of Proteins in Living Bacteria with In-Cell NMR Experiments

2008 ◽  
pp. 203-214 ◽  
Author(s):  
Volker Dötsch
Keyword(s):  
Living Bacteria

scholarly journals Antibacterial Activity of N,O-Acylated Chitosan Derivative

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 107
Author(s):  
Agnieszka Piegat ◽  
Anna Żywicka ◽  
Agata Niemczyk ◽  
Agata Goszczyńska
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Chitosan Derivative ◽  
Modified Chitosan ◽  
Microdilution Method ◽  
Hydrophobically Modified ◽  
Mechanism Of Interaction ◽  
H Pylori ◽  
Living Bacteria ◽  
Antibacterial Potential

The antibacterial activity of N,O-acylated chitosan derivative with linoleic acid (CH_LA) was tested by disc and well diffusion, agar impregnation and microdilution methods against Staphylococcus aureus, Escherichia coli and Helicobacter pylori strains. Hydrophobically modified chitosan (HMC) was expected to exhibit enhanced antibacterial activity and specific mucin interactions. Although diffusion tests have not indicated the antibacterial potential of chitosan (CH) or CH_LA, the results of the microdilution method demonstrated that tested polymers significantly reduced the amount of living bacteria cells in different concentrations depending on the microorganism. Additionally, CH_LA was characterized by enhanced antibacterial activity compared to CH, which may suggest a different mechanism of interaction with S. aureus and H. pylori. Furthermore, the UV-VIS analysis revealed that the amphiphilic character of derivative led to strong CH_LA–mucin interactions. The study proved the high potential of CH_LA in antibacterial applications, especially for the gastrointestinal tract.

scholarly journals Circulating bacterial signature is linked to metabolic disease and shifts with metabolic alleviation after bariatric surgery

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Rima M. Chakaroun ◽  
Lucas Massier ◽  
Anna Heintz-Buschart ◽  
Nedal Said ◽  
Joerg Fallmann ◽  
...  
Keyword(s):  
Metabolic Disease ◽  
Bariatric Surgery ◽  
Weight Loss ◽  
Metabolic Health ◽  
Rrna Gene ◽  
Clinical Group ◽  
Analytical Control ◽  
Bacterial Dna ◽  
Blood Samples ◽  
Living Bacteria

Abstract Background The microbiome has emerged as an environmental factor contributing to obesity and type 2 diabetes (T2D). Increasing evidence suggests links between circulating bacterial components (i.e., bacterial DNA), cardiometabolic disease, and blunted response to metabolic interventions. In this aspect, thorough next-generation sequencing-based and contaminant-aware approaches are lacking. To address this, we tested whether bacterial DNA could be amplified in the blood of subjects with obesity and high metabolic risk under strict experimental and analytical control and whether a putative bacterial signature is related to metabolic improvement after bariatric surgery. Methods Subjects undergoing bariatric surgery were recruited into sex- and BMI-matched subgroups with (n = 24) or without T2D (n = 24). Bacterial DNA in the blood was quantified and prokaryotic 16S rRNA gene amplicons were sequenced. A contaminant-aware approach was applied to derive a compositional microbial signature from bacterial sequences in all subjects at baseline and at 3 and 12 months after surgery. We modeled associations between bacterial load and composition with host metabolic and anthropometric markers. We further tested whether compositional shifts were related to weight loss response and T2D remission. Lastly, bacteria were visualized in blood samples using catalyzed reporter deposition (CARD)-fluorescence in situ hybridization (FISH). Results The contaminant-aware blood bacterial signature was associated with metabolic health. Based on bacterial phyla and genera detected in the blood samples, a metabolic syndrome classification index score was derived and shown to robustly classify subjects along their actual clinical group. T2D was characterized by decreased bacterial richness and loss of genera associated with improved metabolic health. Weight loss and metabolic improvement following bariatric surgery were associated with an early and stable increase of these genera in parallel with improvements in key cardiometabolic risk parameters. CARD-FISH allowed the detection of living bacteria in blood samples in obesity. Conclusions We show that the circulating bacterial signature reflects metabolic disease and its improvement after bariatric surgery. Our work provides contaminant-aware evidence for the presence of living bacteria in the blood and suggests a putative crosstalk between components of the blood and metabolism in metabolic health regulation.

Label-Free Separation of Living Bacteria and Eukaryotic Cells by a Novel Microfluidic Dielectrophoresis Integrated Chip System

2021 ◽  
Author(s):  
Kyoichi Oshiro ◽  
Yoshikazu Wakizaka ◽  
Masayo Takano ◽  
Takayuki Itoi ◽  
Hiroki Ohge ◽  
...  
Keyword(s):  
Eukaryotic Cells ◽  
Label Free ◽  
Living Bacteria

Real-time activity assays of β-lactamases in living bacterial cells: application to the inhibition of antibiotic-resistant E. coli strains

2017 ◽  
Vol 13 (11) ◽  
pp. 2323-2327 ◽  
Author(s):  
Ying Ge ◽  
Ya-Jun Zhou ◽  
Ke-Wu Yang ◽  
Yi-Lin Zhang ◽  
Yang Xiang ◽  
...  
Keyword(s):  
Real Time ◽  
Biological Systems ◽  
Bacterial Cells ◽  
Antibiotic Resistant ◽  
Time Activity ◽  
E Coli ◽  
Living Bacteria

A UV-Vis approach is reported for activity assays and inhibition of β-lactamases in complex biological systems of living bacteria.

scholarly journals Label-Free Visualization of Carbapenemase Activity in Living Bacteria

2018 ◽  
Vol 57 (52) ◽  
pp. 17120-17124 ◽  
Author(s):  
Ye Zhang ◽  
Jin-E Lei ◽  
Yuan He ◽  
Jianhua Yang ◽  
Wenjing Wang ◽  
...  
Keyword(s):  
Label Free ◽  
Living Bacteria

scholarly journals Comparative Study on Antioxidant Capacity of Self-fermenting Enzyme and Commercial Enzyme

2021 ◽  
Vol 251 ◽  
pp. 02032
Author(s):  
Fenghong Liu ◽  
Xianhao Cheng ◽  
Jing Miu ◽  
Xiaotong Cui ◽  
Xiaojuan Gao ◽  
...  
Keyword(s):  
Free Radicals ◽  
Comparative Study ◽  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Antioxidant Ability ◽  
Commercial Enzyme ◽  
Visible Spectrophotometry ◽  
Uv Visible ◽  
Living Bacteria

In order to provide consumers with a more reasonable method of using Enzyme, UV-visible spectrophotometry was used to study the antioxidant ability of self-fermenting Enzyme and commercial Enzyme. By measuring the ability of fourteen kinds of self-fermenting Enzyme and three commercial Enzyme to scavenge DPPH free radicals, superoxide anion and hydroxyl radicals and the number of living bacteria, the following conclusions were drawn: (1) Commercial Enzyme has more properties than self-fermenting Enzyme of antioxidant capacity; (2) Compound fruit Enzyme has stronger antioxidant ability than single fruit Enzyme; (3) Self-fermenting Enzyme is not sterilized including more living bacteria; (4) Commercial Enzyme has a higher drinking value than self-fermenting Enzyme.

scholarly journals Opening a next-generation black box: ecological trends for hundreds of species-like taxa uncovered within a single bacterial >99% 16S rRNA operational taxonomic unit

2021 ◽  
Author(s):  
Martin Hahn ◽  
Andrea Huemer ◽  
Alexandra Pitt ◽  
Matthias Hoetzinger
Keyword(s):  
16S Rrna ◽  
Sequence Similarity ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Taxon Richness ◽  
Large Set ◽  
Free Living ◽  
Environmental Distribution ◽  
Living Bacteria

Current knowledge on environmental distribution and taxon richness of free-living bacteria is mainly based on cultivation-independent investigations employing 16S rRNA gene sequencing methods. Yet, 16S rRNA genes are evolutionarily rather conserved, resulting in limited taxonomic and ecological resolutions provided by this marker. We used a faster evolving protein-encoding marker to reveal ecological patterns hidden within a single OTU defined by >99% 16S rRNA sequence similarity. The studied taxon, subcluster PnecC of the genus Polynucleobacter, represents a ubiquitous group of planktonic freshwater bacteria with cosmopolitan distribution, which is very frequently detected by diversity surveys of freshwater systems. Based on genome taxonomy and a large set of genome sequences, a sequence similarity threshold for delineation of species-like taxa could be established. In total, 600 species-like taxa were detected in 99 freshwater habitats scattered across three regions representing a latitudinal range of 3400 km (42°N to 71°N) and a pH gradient of 4.2 to 8.6. Besides the unexpectedly high richness, the increased taxonomic resolution revealed structuring of Polynucleobacter communities by a couple of macroecological trends, which was previously only demonstrated for phylogenetically much broader groups of bacteria. A unexpected pattern was the almost complete compositional separation of Polynucleobacter communities of Ca-rich and Ca-poor habitats, which strongly resembled the vicariance of plant species on silicate and limestone soils. The presented new cultivation-independent approach opened a window to an incredible, previously unseen diversity, and enables investigations aiming on deeper understanding of how environmental conditions shape bacterial communities and drive evolution of free-living bacteria.

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