scholarly journals Non-rigid Registration of 3D Multi-channel Microscopy Images of Cell Nuclei

Author(s):  
Siwei Yang ◽  
Daniela Köhler ◽  
Kathrin Teller ◽  
Thomas Cremer ◽  
Patricia Le Baccon ◽  
...  
Author(s):  
Siwei Yang ◽  
Daniela Köhler ◽  
Kathrin Teller ◽  
Thomas Cremer ◽  
Roland Eils ◽  
...  

2016 ◽  
Vol 23 (6) ◽  
pp. 1490-1497 ◽  
Author(s):  
Ian Robinson ◽  
Yang Yang ◽  
Fucai Zhang ◽  
Christophe Lynch ◽  
Mohammed Yusuf ◽  
...  

Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.


2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Stephan Wienert ◽  
Daniel Heim ◽  
Kai Saeger ◽  
Albrecht Stenzinger ◽  
Michael Beil ◽  
...  

2011 ◽  
Author(s):  
Kevin S. Lorenz ◽  
Paul Salama ◽  
Kenneth W. Dunn ◽  
Edward J. Delp

2020 ◽  
Vol 75 (1) ◽  
pp. 147-162
Author(s):  
MarKjoe Olunna Uba ◽  
Karol Mikula ◽  
Zuzana Krivá ◽  
Hanh Nguyen ◽  
Thierry Savy ◽  
...  

AbstractIn this work, we focused on 3D image segmentation where the segmented surface is reconstructed by the use of 3D digital image information and information from thresholded 3D image in a local neighbourhood. To this end, we applied a mathematical model based on the level set formulation and numerical method which is based on the so-called reduced diamond cell approach. The segmentation approach is based on surface evolution governed by a nonlinear PDE, the modified subjective surface equation. This is done by defining the input to the edge detector function as the weighted sum of norm of presmoothed 3D image and norm of presmoothed thresholded 3D image in a local neighbourhood. For the numerical discretization, we used a semi-implicit finite volume scheme. The method was applied to real data representing 3D microscopy images of cell nuclei within the zebrafish pectoral fin.


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