HVEM Grid: Experiences in Constructing an Electron Microscopy Grid

2006 ◽  
pp. 1159-1162 ◽  
Author(s):  
Hyuck Han ◽  
Hyungsoo Jung ◽  
Heon Y. Yeom ◽  
Hee S. Kweon ◽  
Jysoo Lee
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopy Grid

Tailoring surface acoustic wave atomisation for cryo-electron microscopy sample preparation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1378-1385 ◽  
Author(s):  
Dariush Ashtiani ◽  
Alex de Marco ◽  
Adrian Neild
Keyword(s):  
Electron Microscopy ◽  
Fluid Flow ◽  
Sample Preparation ◽  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Flow Rate ◽  
Fluid Flow Rate ◽  
Cryo Electron Microscopy ◽  
Electron Microscopy Grid

Surface acoustic wave (SAW) atomisation is investigated in the context of cryo electron microscopy grid preparation. Here, the primary requirements are a reproducible and narrow plume of droplets delivering a low fluid flow rate.

scholarly journals Development of a deep learning-based method to identify “good” regions of a cryo-electron microscopy grid

2020 ◽  
Vol 12 (2) ◽  
pp. 349-354 ◽  
Author(s):  
Yuichi Yokoyama ◽  
Tohru Terada ◽  
Kentaro Shimizu ◽  
Kouki Nishikawa ◽  
Daisuke Kozai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Deep Learning ◽  
Cryo Electron Microscopy ◽  
Electron Microscopy Grid

Two-dimensional crystallization of proteins on lipid monolayers at the air–water interface and transfer to an electron microscopy grid

1999 ◽  
Vol 196 (2-4) ◽  
pp. 456-470 ◽  
Author(s):  
Alain Brisson ◽  
Wilma Bergsma-Schutter ◽  
Frank Oling ◽  
Olivier Lambert ◽  
Ilya Reviakine
Keyword(s):  
Electron Microscopy ◽  
Lipid Monolayers ◽  
Water Interface ◽  
Two Dimensional ◽  
Air Water Interface ◽  
Electron Microscopy Grid

scholarly journals Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

2017 ◽  
Vol 197 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Stefan A. Arnold ◽  
Stefan Albiez ◽  
Andrej Bieri ◽  
Anastasia Syntychaki ◽  
Ricardo Adaixo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Single Cell ◽  
Cell Extracts ◽  
Cryo Electron Microscopy ◽  
Electron Microscopy Grid

scholarly journals Cryotop and development of vitrified immature bovine oocytes

2011 ◽  
Vol 63 (1) ◽  
pp. 67-73 ◽  
Author(s):  
H Hajarian ◽  
H Wahid ◽  
Y Rosnina ◽  
M Daliri ◽  
M Dashtizad ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Polar Body ◽  
Cumulus Cells ◽  
Control Group ◽  
Significant Difference ◽  
Developmental Rates ◽  
Electron Microscopy Grid ◽  
The Mean ◽  
Polar Body Extrusion ◽  
Bovine Oocytes

The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes using OPS and EMG showed 26 and 32%; and 35 and 46% of PB and MII rates, respectively. The highest survivability resulted from Cryotop and EMG groups and no significant difference was found between them. Vitrified oocytes using Cryotop had the highest cleavage and blastocyst rates. All of the mean rates for vitrified immature oocytes were significantly lower than that of control group (P<0.05). The results of this study showed the superiority of Cryotop device for vitrification of immature bovine oocytes

scholarly journals A Cryo-EM Grid Preparation Device for Time-Resolved Structural Studies

2019 ◽  
Author(s):  
Dimitrios Kontziampasis ◽  
David P. Klebl ◽  
Matthew G. Iadanza ◽  
Charlotte A. Scarff ◽  
Florian Kopf ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Structural Biology ◽  
Conformational Changes ◽  
Structural Studies ◽  
Protein Conformations ◽  
Time Resolved ◽  
Rapid Mixing ◽  
Cryo Electron Microscopy ◽  
Electron Microscopy Grid ◽  
Functional Mechanisms

AbstractStructural biology generally provides static snapshots of protein conformations that can inform on the functional mechanisms of biological systems. Time-resolved structural biology provides a means to visualise, at near-atomic resolution, the dynamic conformational changes that macromolecules undergo as they function. Recent advances in the resolution obtainable by electron microscopy (EM) and the broad range of samples that can be studied makes it ideally suited to time-resolved studies. Here we describe a cryo-electron microscopy grid preparation device that permits rapid mixing, voltage assisted spraying, and vitrification of samples. We show that the device produces grids of sufficient ice quality to enable data collection from single grids that results in a sub 4 Å reconstruction. Rapid mixing can be achieved by blot and spray or mix and spray approaches with a delay of ~10 ms, providing greater temporal resolution than previously reported approaches.

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