Bioanalytical Application of Peroxidase-Mimicking DNAzymes: Status and Challenges

2017 ◽  
pp. 59-84 ◽  
Author(s):  
J. Kosman ◽  
B. Juskowiak
Keyword(s):  
Bioanalytical Application

scholarly journals A novel quantification platform for point-of-care testing of circulating MicroRNAs based on allosteric spherical nanoprobe

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer (NSCLC), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC. Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield “signal on” fluorescence. In the manner of multipoint fluorescence detection, the target-bound allosteric spherical nanoprobe could provide high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method can distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

scholarly journals Rapid Determination of Isomeric Benzoylpaeoniflorin and Benzoylalbiflorin in Rat Plasma by LC-MS/MS Method

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Chuanqi Zhou ◽  
Xiaoke Wang
Keyword(s):  
Linear Response ◽  
Therapeutic Agent ◽  
Rapid Determination ◽  
Rat Plasma ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Bioanalytical Application ◽  
Tandem Mass Spectrometric

Benzoylpaeoniflorin (BP) is a potential therapeutic agent against oxidative stress related Alzheimer’s disease. In this study, a more rapid, selective, and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to determine BP in rat plasma distinguishing with a monoterpene isomer, benzoylalbiflorin (BA). The method showed a linear response from 1 to 1000 ng/mL (r>0.9950). The precision of the interday and intraday ranged from 2.03 to 12.48% and the accuracy values ranged from −8.00 to 10.33%. Each running of the method could be finished in 4 minutes. The LC-MS/MS method was validated for specificity, linearity, precision, accuracy, recovery, and stability and was found to be acceptable for bioanalytical application. Finally, this fully validated method was successfully applied to a pharmacokinetic study in rats following oral administration.

scholarly journals A Novel Quantification Platform for Point-of-care testing of Circulating MicroRNAs Based on Allosteric Spherical Nanoprobe

2020 ◽  
Author(s):  
Huiyan Tian ◽  
Changjing Yuan ◽  
Yu Liu ◽  
Zhi Li ◽  
Ke Xia ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Reaction Cross ◽  
Detection Sensitivity ◽  
Point Of Care Testing ◽  
Bioanalytical Application ◽  
Target Probe ◽  
Nsclc Patients

Abstract MiRNA-150, a gene regulator that has been revealed to be abnormal expression in non-small cell lung cancer ( NSCLC ), can be regarded as a serum indicator for diagnosis and monitoring of NSCLC . Herein, a new sort of nanoprobe, termed allosteric spherical nanoprobe, was first developed to sense miRNA-150. Compared with conventional hairpin, this new nanoprobe possesses more enrichment capacity and reaction cross section. Structurally, it consists of magnetic nanoparticles and dual-hairpin. In the absence of miRNA-150, the spherical nanoprobes form hairpin structure through DNA self-assembly, which could promote the Förster resonance energy transfer (FRET) of fluorophore (FAM) and quencher (BHQ1) nearby. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active “Y” structure which separated fluorophore and quencher to yield a “signal on” fluorescence. In the manner of multipoint fluorescence detection , the target-bound allosteric spherical nanoprobe could provide a high detection sensitivity with a linear range of 100 fM to 10 nM and a detection limit of 38 fM. More importantly, the proposed method could distinguish the expression of serum miRNA-150 among NSCLC patients and healthy people. Finally, we hoped that the potential bioanalytical application of this nanoprobe strategy will pave the way for point-of-care testing (POCT).

Interface between Nanophotonics and Biotechnology: How the Near-Field Can Boost Proteomics Based on LSPR Nano Sensor

2010 ◽  
Vol 139-141 ◽  
pp. 1554-1557 ◽  
Author(s):  
Xi Xi Huang ◽  
Zhong Cao ◽  
Yong Le Liu ◽  
Yi Min Dai ◽  
Ju Lan Zeng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Near Field ◽  
Visible Region ◽  
High Sensitivity ◽  
Nano Particles ◽  
Localized Surface Plasmon ◽  
Bioanalytical Application

An novel optical nano biosensor based on gold capped nano-particles for detecting binding events between ligands and receptor molecules as well as interactions among proteins without use of labels has been presented in this paper. The optical properties of nano-sized gold particles exhibiting pronounced adsorption in the visible region which called as localized surface plasmon resonance (LSPR) have been exploited, whose peak wavelengths depended exquisitely on the refractive index of the surrounding. In comparison with surface plasmon resonance (SPR) technology, the optical nano biosensor possessed high sensitivity, surprisingly low “bulk effect”, ease of preparation, and low-cost polymer based fabrication, which opened a promising bioanalytical application in practice.

Bioanalytical Application of the Ordered Mesoporous Carbon Modified Electrodes

2008 ◽  
Vol 20 (23) ◽  
pp. 2518-2525 ◽  
Author(s):  
Liande Zhu ◽  
Chunyuan Tian ◽  
Dongxu Yang ◽  
Xiaoyan Jiang ◽  
Ruilan Yang
Keyword(s):  
Mesoporous Carbon ◽  
Modified Electrodes ◽  
Ordered Mesoporous Carbon ◽  
Ordered Mesoporous ◽  
Bioanalytical Application

New Strategies for the Fabrication of Enzyme Electrodes

1995 ◽  
Vol 414 ◽  
Author(s):  
K. Shridhara Alva ◽  
Lynne A. Samuelsont ◽  
Jayant Kumar ◽  
Kenneth A. Marx ◽  
David L. Kaplan ◽  
...  
Keyword(s):  
Optical Properties ◽  
Horseradish Peroxidase ◽  
Electrochemical Properties ◽  
Enzyme Electrodes ◽  
Electrochemical Copolymerization ◽  
Chemical Coupling ◽  
Bioanalytical Application ◽  
Glass Electrodes ◽  
Polymerization Conditions ◽  
New Strategies

AbstractThe electrochemical properties of o-dianisidine have been studied in both aqueous and organic solvents. This aryl diamine has been electrochemically polymerized on ITO coated glass electrodes and it was determined that the optical properties of these films are influenced by the polymerization conditions. The use of this polymer film as substrate for chemical coupling of biomaterials has been demonstrated with the light harvesting protein, phycoerythrin. Fabrication and bioanalytical application of enzyme electrodes by electrochemical copolymerization of odianisidine with the enzymes glucose oxidase and horseradish peroxidase, modified with odianisidineis described.

Fabrication of Gold Cap-Shaped Nano-Particle Films and Sensing Device for Bioanalytical Application

2010 ◽  
Vol 97-101 ◽  
pp. 2188-2191
Author(s):  
Zhong Cao ◽  
Yong Le Liu ◽  
Su Xi Wu ◽  
Fu Chun Gong ◽  
Shu Long ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Hybridization ◽  
Gold Surface ◽  
Nano Particles ◽  
Nano Particle ◽  
Silica Nanospheres ◽  
Sensing System ◽  
Bioanalytical Application ◽  
Hybridization Reaction ◽  
Carboxymethyl Dextran

The nano biosensors based on gold cap-shaped nano-particles like polystyrene and silica nanospheres have been successfully prepared on the substrates of disposable plate (96 wells) and miniplate (72 wells). The sensing film of carboxymethyl dextran layer was modified on the gold surface of the cap-shaped nano-particles. The corresponding micro-device with four channel of optical sensing system has been well constructed and applied to detection of anti-avidin D protein and biotinylated DNA molecules. The DNA hybridization reaction has also been real-time and parallel monitored in four channels, showing that the device possessed a nice reproducibility, which opens a promising bioanalytical application in practice.

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