CHO Glycosylation Mutants as Potential Host Cells to Produce Therapeutic Proteins with Enhanced Efficacy

The ecology of Chytridium deltanum and other fungus parasites on Oocystis spp.

Canadian Journal of Botany ◽

10.1139/b71-016 ◽

1971 ◽

Vol 49 (1) ◽

pp. 75-87 ◽

Cited By ~ 12

Author(s):

Margaret J. Masters

Keyword(s):

Field Studies ◽

Host Population ◽

Host Cells ◽

Aquatic Fungus ◽

Potential Host ◽

Large Numbers ◽

Synchronous Development

Consideration of the population curves of Oocystis crassa and O. lacustris in Lake Manitoba during the summers of 1966 and 1967, and July 1968, indicated that the aquatic fungus Chytridium deltanum was able in two instances to attack growing populations of these algae. This strongly suggested that the fungus was a parasite. However, in July 1965, in Cadham Bay, the fungus bloomed as the host population stopped growing and began to decline. Probably the host cells were slightly senescent at that time and thus more susceptible to fungus attack. Consideration of the composition of the fungus population showed that during one epidemic at least, large numbers of zoospores were released every 7 to 9 days. This suggested a nearly synchronous development of the fungus population. It was also observed that zoospores, able to encyst and successfully infect one host, were sometimes unable to attack another potential host present at the same time. In 1966 and 1967 the fungus appeared a few days after the water had reached 25C. Comparison of culture data for the algae and field studies indicated that Chytridium deltanum most commonly grew on the algae at temperatures above the optimum for the algae.

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High Voltage Electron Microscopy of Viral Inclusion Bodies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002117 ◽

1980 ◽

Vol 38 ◽

pp. 486-487 ◽

Cited By ~ 1

Author(s):

H.M. Mazzone ◽

W.F. Engler ◽

G. Wray ◽

A. Szirmae ◽

J. Conroy ◽

...

Keyword(s):

New York ◽

Environmental Protection Agency ◽

Inclusion Bodies ◽

Host Cells ◽

High Voltage Electron Microscopy ◽

Voltage Electron ◽

Viral Particles ◽

Pest Insects ◽

Viral Inclusion ◽

Insect Gut

Viral inclusion bodies isolated from infected pest insects are being evaluated by the U.S. Dept. of Agriculture as biological insecticides against their hosts. Our research on these inclusion bodies constitutes part of an effort to support their approval by the Environmental Protection Agency as insect control agents. The inclusion bodies in this study are polyhedral in shape and contain rod-shaped viral particles. When ingested by pest insects, the inclusion bodies are broken down in the insect gut and release the viral particles which infect and multiply in the nuclei of host cells. These viruses are termed nucleopolyhedrosis viruses (NPV) and are representatives of the baculoviruses (Wildy, P. 1971 IN J.L. Melnick, ed., Monographs in Virology, vol. 5, S.Karger, New York).

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