Cartilage Engineering from Mesenchymal Stem Cells

2010 ◽  
pp. 163-200 ◽  
Author(s):  
C. Goepfert ◽  
A. Slobodianski ◽  
A. F. Schilling ◽  
P. Adamietz ◽  
R. Pörtner
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Engineering

scholarly journals Differentiation of human adult mesenchymal stem cells in chondrocytes for cartilage engineering

2012 ◽  
Vol 20 ◽  
pp. S278 ◽  
Author(s):  
D. Ollitrault ◽  
F. Legendre ◽  
T. Gomez-Leduc ◽  
M. Hervieu ◽  
M. Bouyoucef ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Engineering ◽  
Human Adult ◽  
Adult Mesenchymal Stem Cells

scholarly journals Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering Using Human Mesenchymal Stem Cells and Bioreactor

2008 ◽  
Vol 149 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Sasa Janjanin ◽  
Wan-Ju Li ◽  
Meredith T. Morgan ◽  
Rabie M. Shanti ◽  
Rocky S. Tuan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cartilage Engineering ◽  
Nanofiber Scaffold

scholarly journals Magnetic Nanoparticles and Magnetic Field Exposure Enhances Chondrogenesis of Human Adipose Derived Mesenchymal Stem Cells But Not of Wharton Jelly Mesenchymal Stem Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Luminita Labusca ◽  
Dumitru-Daniel Herea ◽  
Anca Emanuela Minuti ◽  
Cristina Stavila ◽  
Camelia Danceanu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Nanoparticles ◽  
Cellular Senescence ◽  
Field Exposure ◽  
Differentiation Potential ◽  
Cartilage Engineering

Purpose: Iron oxide based magnetic nanoparticles (MNP) are versatile tools in biology and medicine. Adipose derived mesenchymal stem cells (ADSC) and Wharton Jelly mesenchymal stem cells (WJMSC) are currently tested in different strategies for regenerative regenerative medicine (RM) purposes. Their superiority compared to other mesenchymal stem cell consists in larger availability, and superior proliferative and differentiation potential. Magnetic field (MF) exposure of MNP-loaded ADSC has been proposed as a method to deliver mechanical stimulation for increasing conversion to musculoskeletal lineages. In this study, we investigated comparatively chondrogenic conversion of ADSC-MNP and WJMSC with or without MF exposure in order to identify the most appropriate cell source and differentiation protocol for future cartilage engineering strategies.Methods: Human primary ADSC and WJMSC from various donors were loaded with proprietary uncoated MNP. The in vitro effect on proliferation and cellular senescence (beta galactosidase assay) in long term culture was assessed. In vitro chondrogenic differentiation in pellet culture system, with or without MF exposure, was assessed using pellet histology (Safranin O staining) as well as quantitative evaluation of glycosaminoglycan (GAG) deposition per cell.Results: ADSC-MNP complexes displayed superior proliferative capability and decreased senescence after long term (28 days) culture in vitro compared to non-loaded ADSC and to WJMSC-MNP. Significant increase in chondrogenesis conversion in terms of GAG/cell ratio could be observed in ADSC-MNP. MF exposure increased glycosaminoglycan deposition in MNP-loaded ADSC, but not in WJMSC.Conclusion: ADSC-MNP display decreased cellular senescence and superior chondrogenic capability in vitro compared to non-loaded cells as well as to WJMSC-MNP. MF exposure further increases ADSC-MNP chondrogenesis in ADSC, but not in WJMSC. Loading ADSC with MNP can derive a successful procedure for obtaining improved chondrogenesis in ADSC. Further in vivo studies are needed to confirm the utility of ADSC-MNP complexes for cartilage engineering.

Chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells in type I collagen-hydrogel for cartilage engineering

Injury ◽  
2013 ◽  
Vol 44 (4) ◽  
pp. 540-549 ◽  
Author(s):  
Xuebin Chen ◽  
Fang Zhang ◽  
Xinhong He ◽  
Yinsheng Xu ◽  
Zhiping Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Chondrogenic Differentiation ◽  
Type I Collagen ◽  
Type I ◽  
Cartilage Engineering ◽  
Collagen Hydrogel

scholarly journals Characterization and use of equine bone marrow mesenchymal stem cells in horse cartilage engineering

2014 ◽  
Vol 22 ◽  
pp. S489
Author(s):  
T. Branly ◽  
M. Hervieu ◽  
S. Jacquet ◽  
L. Bertoni ◽  
T. Gomez-Leduc ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cartilage Engineering ◽  
Marrow Mesenchymal Stem Cells ◽  
Equine Bone

scholarly journals Adult mesenchymal stem cells for bone and cartilage engineering: effect of scaffold materials

10.4081/1208 ◽  
2009 ◽  
Vol 52 (3) ◽  
pp. 169 ◽  
Author(s):  
A Gigante ◽  
S Manzotti ◽  
C Bevilacqua ◽  
M Orciani ◽  
R Di Primio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Engineering ◽  
Adult Mesenchymal Stem Cells ◽  
Scaffold Materials ◽  
And Cartilage

Mesenchymal stem cells for cartilage engineering

2012 ◽  
Vol 22 (1-3) ◽  
pp. 69-80 ◽  
Author(s):  
C. Huselstein ◽  
Y. Li ◽  
X. He
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Engineering
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