Design, Synthesis, and Biological Application of Fluorescent Sensor Molecules for Cellular Imaging

2009 ◽  
pp. 63-78 ◽  
Author(s):  
Kazuya Kikuchi
Keyword(s):  
Fluorescent Sensor ◽  
Cellular Imaging ◽  
Biological Application ◽  
Design Synthesis ◽  
Sensor Molecules

Design, synthesis, biological evaluation and cellular imaging of imidazo[4,5-b]pyridine derivatives as potent and selective TAM inhibitors

2018 ◽  
Vol 26 (20) ◽  
pp. 5510-5530 ◽  
Author(s):  
Tom Baladi ◽  
Jessy Aziz ◽  
Florent Dufour ◽  
Valentina Abet ◽  
Véronique Stoven ◽  
...  
Keyword(s):  
Biological Evaluation ◽  
Cellular Imaging ◽  
Pyridine Derivatives ◽  
Design Synthesis

Design, synthesis, and evaluation of an activity-based probe for cellular imaging of monoamine oxidases

2011 ◽  
Vol 21 (11) ◽  
pp. 3858-3862 ◽  
Author(s):  
Wei Shen ◽  
Shaobo Long ◽  
Shian Yu ◽  
Weiwei Chen ◽  
Qing Zhu
Keyword(s):  
Cellular Imaging ◽  
Design Synthesis ◽  
Monoamine Oxidases

scholarly journals An Activity-Based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells

2020 ◽  
Author(s):  
Regina A. Baglia ◽  
Kira Mills ◽  
Koushambi Mitra ◽  
Jasmine Tutol ◽  
Darby Ball ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Biological Application ◽  
Intact Cells ◽  
Phenol Sulfotransferase ◽  
Endogenous Metabolites ◽  
Direct Readout ◽  
Inorganic Sulfate

<p>The biological activation and incorporation of inorganic sulfate proceeds via a process known as sulfurylation. Transfer of a sulfuryl moiety from the activated sulfate donor, 3’-phosphoadenosine-5’-phosphosulfate (PAPS), to hydroxy-containing substrates by human phenol sulfotransferases (SULT1 family) alters substrate solubility and charge to affect the metabolism of endogenous metabolites, xenobiotics, and drugs. Current methods to monitor SULT1 activity in living cells primarily rely on radiolabeling and/or cell extractions, but these methods do not provide a direct readout of enzyme activity with a dynamic, temporally resolved spatial map in live, intact cells. To fill this gap, here, we present the development, computational modeling, <i>in vitro</i> enzymology, and biological application of Sulfotransferase Sensor-3, STS-3, an activity-based fluorescent sensor for SULT1A1, the most widely expressed and promiscuous SULT1 isoform. </p>

scholarly journals An Activity-Based Fluorescent Sensor for the Detection of the Phenol Sulfotransferase SULT1A1 in Living Cells

2020 ◽  
Author(s):  
Regina A. Baglia ◽  
Kira Mills ◽  
Koushambi Mitra ◽  
Jasmine Tutol ◽  
Darby Ball ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Biological Application ◽  
Intact Cells ◽  
Phenol Sulfotransferase ◽  
Endogenous Metabolites ◽  
Direct Readout ◽  
Inorganic Sulfate

<p>The biological activation and incorporation of inorganic sulfate proceeds via a process known as sulfurylation. Transfer of a sulfuryl moiety from the activated sulfate donor, 3’-phosphoadenosine-5’-phosphosulfate (PAPS), to hydroxy-containing substrates by human phenol sulfotransferases (SULT1 family) alters substrate solubility and charge to affect the metabolism of endogenous metabolites, xenobiotics, and drugs. Current methods to monitor SULT1 activity in living cells primarily rely on radiolabeling and/or cell extractions, but these methods do not provide a direct readout of enzyme activity with a dynamic, temporally resolved spatial map in live, intact cells. To fill this gap, here, we present the development, computational modeling, <i>in vitro</i> enzymology, and biological application of Sulfotransferase Sensor-3, STS-3, an activity-based fluorescent sensor for SULT1A1, the most widely expressed and promiscuous SULT1 isoform. </p>

Synthesis and in vitro assessment of a bifunctional closomer probe for fluorine (19F) magnetic resonance and optical bimodal cellular imaging

2014 ◽  
Vol 50 (43) ◽  
pp. 5793-5795 ◽  
Author(s):  
Lalit N. Goswami ◽  
Aslam A. Khan ◽  
Satish S. Jalisatgi ◽  
M. Frederick Hawthorne
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Fluorescence Detection ◽  
Cellular Imaging ◽  
Resonance Spectroscopy ◽  
Design Synthesis ◽  
Imaging Probe ◽  
In Vitro Assessment

The design, synthesis and in vitro assessment of a closo-borane based bifunctional imaging probe for dual fluorine (19F) magnetic resonance spectroscopy (19F-MRS) and fluorescence detection is reported.

scholarly journals Design, Synthesis, and Biological Application of Novel Photoaffinity Probes of Dihydropyridine Derivatives, BAY R3401

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2394
Author(s):  
Liying Zhang ◽  
Zhiwei Yan ◽  
Youde Wang ◽  
Chengjun Song ◽  
Guangxin Miao
Keyword(s):  
Hepg2 Cells ◽  
Human Liver ◽  
Molecular Mechanisms ◽  
Biological Application ◽  
Design Synthesis ◽  
Target Proteins ◽  
Promising Tool ◽  
Photoaffinity Probes ◽  
Ic50 Values ◽  
Dihydropyridine Derivatives

To explore the molecular mechanisms of BAY R3401, four types of novel photoaffinity probes bearing different secondary tags were synthesized. Their potency for glycogenolysis was evaluated in primary human liver HL-7702 cells and HepG2 cells. Probe 2d showed the best activity in primary human liver HL-7702 cells and HepG2 cells, with IC50 values of 4.45 μM and 28.49 μM, respectively. Likewise, probe 5d showed IC50 values of 6.46 μM in primary human liver HL-7702 cells and 15.29 μM in HepG2 cells, respectively. Photoaffinity labeling experiments were also performed and protein bands larger than 170 kDa were specifically tagged by probe 2d. The results suggest that the synthesized probe 2d might be a very promising tool for the isolation of the target proteins of BAY R3401.

Optically active crown ether-based fluorescent sensor molecules: A mini-review

Chirality ◽  
2018 ◽  
Vol 31 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Ildikó Móczár ◽  
Péter Huszthy
Keyword(s):  
Crown Ether ◽  
Fluorescent Sensor ◽  
Optically Active ◽  
Sensor Molecules
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