scholarly journals A Minimal Genome Simian Foamy Virus Type 1 Vector System with Efficient Gene Transfer

Virology ◽  
2002 ◽  
Vol 302 (2) ◽  
pp. 236-244 ◽  
Author(s):  
Jeonghae Park ◽  
Peter E. Nadeau ◽  
Ayalew Mergia
Keyword(s):  
Gene Transfer ◽  
Virus Type ◽  
Foamy Virus ◽  
Vector System ◽  
Simian Foamy Virus ◽  
Minimal Genome ◽  
Efficient Gene

scholarly journals cis-Acting Sequences Required for Simian Foamy Virus Type 1 Vectors

1998 ◽  
Vol 72 (4) ◽  
pp. 3451-3454 ◽  
Author(s):  
Min Wu ◽  
Soumya Chari ◽  
Tina Yanchis ◽  
Ayalew Mergia
Keyword(s):  
Gene Transfer ◽  
Virus Type ◽  
Foamy Virus ◽  
Gene Promoter ◽  
Early Gene ◽  
Leader Region ◽  
Simian Foamy Virus ◽  
Cis Acting ◽  
Vector Construction

ABSTRACT We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of a deletion mutation in the leader region between the 5′ long terminal repeat and the start of the gag gene at position 1659 to 1694 completely abrogated gene transfer by the SFV-1 vector. Deletion of 39 nucleotides from position 1692 to 1731 in the leader region resulted in a significant reduction in the transducing-particle titer. Furthermore, we have identified a second cis-acting element located at the 3′ end of the pol gene between position 6486 and 6975 to be critical for SFV-1 vector transduction. These results identify the two important cis-acting elements required for SFV-1 vector construction, and the finding of a cis-acting element in the pol gene is unique among retroviruses.

scholarly journals Packaging Cell Lines for Simian Foamy Virus Type 1 Vectors

1999 ◽  
Vol 73 (5) ◽  
pp. 4498-4501 ◽  
Author(s):  
Min Wu ◽  
Ayalew Mergia
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Virus Type ◽  
Foamy Virus ◽  
Vector System ◽  
Simian Foamy Virus ◽  
Packaging Cell Line ◽  
Packaging Cell ◽  
Foamy Viruses

ABSTRACT Foamy viruses are nonpathogenic retroviruses that offer several unique opportunities for gene transfer in various cell types from different species. We have previously demonstrated the utility of simian foamy virus type 1 (SFV-1) as a vector system by transient expression assay (M. Wu et al., J. Virol. 72:3451–3454, 1998). In this report, we describe the first stable packaging cell lines for foamy virus vectors based on SFV-1. We developed two packaging cell lines in which the helper DNA is placed under the control of either a constitutive cytomegalovirus (CMV) immediate-early gene or inducible tetracycline promoter for expression. Although the constitutive packaging expressing cell line had a higher copy number of packaging DNA, the inducible packaging cell line produced four times more vector particles. This result suggested that the structural gene products in the constitutively expressing packaging cell line were expressed at a level that is not toxic to the cells, and thus vector production was reduced. The SFV-1 vector in the presence of vesicular stomatitis virus envelope protein G (VSV-G) produced an insignificant level of transduction, indicating that foamy viruses could not be pseudotyped with VSV-G to generate high-titer vectors. The availability of stable packaging cell lines represents a step toward the use of an SFV-1 vector delivery system that will allow scaled-up production of vector stocks for gene therapy.

scholarly journals Dual Simian Foamy Virus/Human Immunodeficiency Virus Type 1 Infections in Persons from Côte d’Ivoire

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157709 ◽  
Author(s):  
William M. Switzer ◽  
Shaohua Tang ◽  
HaoQiang Zheng ◽  
Anupama Shankar ◽  
Patrick S. Sprinkle ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Foamy Virus ◽  
Simian Foamy Virus ◽  
Cote D’Ivoire ◽  
Immunodeficiency Virus

Sequence analysis of the simian foamy virus type 1 genome

Gene ◽  
1991 ◽  
Vol 101 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Jean-Jacques Kupiec ◽  
Alan Kay ◽  
Maud Hayat ◽  
Rodica Ravier ◽  
Jorge Périés ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Virus Type ◽  
Foamy Virus ◽  
Simian Foamy Virus

Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1.

1983 ◽  
Vol 47 (1) ◽  
pp. 249-252 ◽  
Author(s):  
A B Benzair ◽  
A Rhodes-Feuillette ◽  
R Emanoil-Ravicovitch ◽  
J Peries
Keyword(s):  
Reverse Transcriptase ◽  
Virus Type ◽  
Foamy Virus ◽  
Rnase H ◽  
Simian Foamy Virus

scholarly journals Purification and Characterization of the Major Envelope Glycoprotein of Simian Foamy Virus Type 1

1985 ◽  
Vol 66 (7) ◽  
pp. 1449-1455 ◽  
Author(s):  
A.-B. Benzair ◽  
A. Rhodes-Feuillette ◽  
J. Lasneret ◽  
R. Emanoil-Ravier ◽  
J. Peries
Keyword(s):  
Virus Type ◽  
Envelope Glycoprotein ◽  
Foamy Virus ◽  
Purification And Characterization ◽  
Simian Foamy Virus

Efficient gene transfer to human squamous cell carcinomas by the herpes simplex virus type 1 amplicon vector

1998 ◽  
Vol 176 (5) ◽  
pp. 404-408 ◽  
Author(s):  
John F. Carew ◽  
Howard Federoff ◽  
Marc Halterman ◽  
Dennis H. Kraus ◽  
Howard Savage ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transfer ◽  
Virus Type ◽  
Squamous Cell ◽  
Herpes Simplex Virus Type ◽  
Squamous Cell Carcinomas ◽  
Efficient Gene ◽  
Simplex Virus

scholarly journals Mutational Analysis of the 5′ Leader Region of Simian Foamy Virus Type 1

Virology ◽  
2000 ◽  
Vol 274 (1) ◽  
pp. 203-212 ◽  
Author(s):  
Jeonghae Park ◽  
Ayalew Mergia
Keyword(s):  
Virus Type ◽  
Mutational Analysis ◽  
Foamy Virus ◽  
Leader Region ◽  
Simian Foamy Virus

scholarly journals Functional domains of the simian foamy virus type 1 transcriptional transactivator (Taf).

1993 ◽  
Vol 67 (8) ◽  
pp. 4598-4604 ◽  
Author(s):  
A Mergia ◽  
L W Renshaw-Gegg ◽  
M W Stout ◽  
R Renne ◽  
O Herchenröeder
Keyword(s):  
Virus Type ◽  
Foamy Virus ◽  
Functional Domains ◽  
Simian Foamy Virus ◽  
Transcriptional Transactivator
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