scholarly journals Expression, Purification, and Characterization of the RNA 5′-Triphosphatase Activity of Dengue Virus Type 2 Nonstructural Protein 3

Virology ◽  
2002 ◽  
Vol 299 (1) ◽  
pp. 122-132 ◽  
Author(s):  
Greg Bartelma ◽  
R. Padmanabhan
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Nonstructural Protein ◽  
Purification And Characterization ◽  
Nonstructural Protein 3 ◽  
Dengue Virus Type 2

Establishment and characterization of dengue virus type 2 nonstructural protein 1 specific T cell lines

2010 ◽  
Vol 33 (6) ◽  
pp. e75-e80 ◽  
Author(s):  
Yang Xiao-meng ◽  
Jiang Li-fang ◽  
Tang Yun-xia ◽  
Yin Yue ◽  
Liu Wen-quan ◽  
...  
Keyword(s):  
T Cell ◽  
Dengue Virus ◽  
Cell Lines ◽  
Virus Type ◽  
Nonstructural Protein ◽  
Nonstructural Protein 1 ◽  
T Cell Lines ◽  
Dengue Virus Type 2

Antiviral activity of Faramea bahiensis leaves on dengue virus type-2 and characterization of a new antiviral flavanone glycoside

2017 ◽  
Vol 19 ◽  
pp. 220-225 ◽  
Author(s):  
Adriana C. Nascimento ◽  
Ligia M.M. Valente ◽  
Mário Gomes ◽  
Rodolfo S. Barboza ◽  
Thiago Wolff ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Type ◽  
Flavanone Glycoside ◽  
Dengue Virus Type 2

scholarly journals Genetic and phenotypic characterization of sylvatic dengue virus type 2 strains

Virology ◽  
2008 ◽  
Vol 377 (2) ◽  
pp. 296-307 ◽  
Author(s):  
Nikos Vasilakis ◽  
Eric B. Fokam ◽  
Christopher T. Hanson ◽  
Ethan Weinberg ◽  
Amadou A. Sall ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Phenotypic Characterization ◽  
Dengue Virus Type 2

Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2

2006 ◽  
Vol 139 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Chie Aoki ◽  
Kazuya I.P.J. Hidari ◽  
Saki Itonori ◽  
Akihiro Yamada ◽  
Naonori Takahashi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Identification And Characterization ◽  
Dengue Virus Type 2

scholarly journals Mutagenesis of the Dengue Virus Type 2 NS3 Protein within and outside Helicase Motifs: Effects on Enzyme Activity and Virus Replication

2001 ◽  
Vol 75 (20) ◽  
pp. 9633-9643 ◽  
Author(s):  
Anita E. Matusan ◽  
Melinda J. Pryor ◽  
Andrew D. Davidson ◽  
Peter J. Wright
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Virus Type ◽  
Nonstructural Protein ◽  
Serine Proteinase ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Helicase Activity ◽  
Dengue Virus Type 2

ABSTRACT The protein NS3 of Dengue virus type 2 (DEN-2) is the second largest nonstructural protein specified by the virus and is known to possess multiple enzymatic activities, including a serine proteinase located in the N-terminal region and an NTPase-helicase in the remaining 70% of the protein. The latter region has seven conserved helicase motifs found in all members of the family Flaviviridae. DEN-2 NS3 lacking the proteinase region was synthesized as a fusion protein with glutathione S-transferase in Escherichia coli. The effects of 10 mutations on ATPase and RNA helicase activity were examined. Residues at four sites within enzyme motifs I, II, and VI were substituted, and six sites outside motifs were altered by clustered charged-to-alanine mutagenesis. The mutations were also tested for their effects on virus replication by incorporation into genomic-length cDNA. Two mutations, both in motif I (G198A and K199A) abolished both ATPase and helicase activity. Two further mutations, one in motif VI (R457A,R458A) and the other a clustered charged-to-alanine substitution at R376KNGK380, abolished helicase activity only. No virus was detected for any mutation which prevented helicase activity, demonstrating the requirement of this enzyme for virus replication. The remaining six mutations resulted in various levels of enzyme activities, and four permitted virus replication. For the two nonreplicating viruses encoding clustered changes at R184KR186 and D436GEE439, we propose that the substituted residues are surface located and that the viruses are defective through altered interaction of NS3 with other components of the viral replication complex. Two of the replicating viruses displayed a temperature-sensitive phenotype. One contained a clustered mutation at D334EE336 and grew too poorly for further characterization. However, virus with an M283F substitution in motif II was examined in a temperature shift experiment (33 to 37°C) and showed reduced RNA synthesis at the higher temperature.

scholarly journals Clinical and Epidemiological Characterization of the First Recognized Outbreak of Dengue Virus-Type 2 in Mozambique, 2014

2016 ◽  
Vol 94 (2) ◽  
pp. 413-416 ◽  
Author(s):  
Marilia Massangaie ◽  
Janusz Paweska ◽  
Celia Chirindza ◽  
Jacqueline Weyer ◽  
Eduardo Samo Gudo ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Epidemiological Characterization ◽  
Dengue Virus Type 2

scholarly journals Characterization of Dengue Virus Type 2: New Insights on the 2010 Brazilian Epidemic

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11811 ◽  
Author(s):  
Camila Malta Romano ◽  
Andréia Manso de Matos ◽  
Evaldo Stanislau A. Araújo ◽  
Lucy Santos Villas-Boas ◽  
Wanessa Cardoso da Silva ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Type 2
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