scholarly journals Characterization of Bombyx mori Nucleopolyhedrovirus orf68 Gene That Encodes a Novel Structural Protein of Budded Virus

Virology ◽  
2002 ◽  
Vol 297 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Masashi Iwanaga ◽  
Masaaki Kurihara ◽  
Masahiko Kobayashi ◽  
WonKyung Kang
Keyword(s):  
Bombyx Mori ◽  
Structural Protein ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Budded Virus

Characterization of a Late Expression Gene of Bombyx mori Nucleopolyhedrovirus

2010 ◽  
Vol 65 (7-8) ◽  
pp. 508-518 ◽  
Author(s):  
Yang Zhou ◽  
Keping Chen ◽  
Qin Yao ◽  
Hongxing Shen ◽  
Guiting Liang ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Infected Cell ◽  
Bombyx Mori ◽  
Polyclonal Antiserum ◽  
Late Gene ◽  
Structural Protein ◽  
Immunofluorescence Analysis ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Late Expression

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF5 (Bm5) is a gene present in many lepidopteran nucleopolyhedroviruses (NPVs), but its function is unknown. In this study, Bm5 was characterized. The transcript of Bm5 was detected 12 - 72 h post infection (p.i.). Polyclonal antiserum raised to a His-BM5 fusion protein recognized BM5 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm5 is a late gene. Immunofluorescence analysis by confocal microscopy showed that the BM5 protein is localized primarily in the cytoplasm. Localization of BM5 in budded virion (BV) and occlusion-derived virion (ODV) by Western analyses demonstrated that BM5 is not a structural protein associated with BV or ODV.

Characterization of Bombyx mori Nucleopolyhedrovirus ORF109 that Encodes a 25-kDa Structural Protein of the Occlusion-Derived Virion

2010 ◽  
Vol 61 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Yang Zhou ◽  
Qin Yao ◽  
Hongxing Shen ◽  
Hengchuan Xia ◽  
Feng Lin ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Structural Protein ◽  
Bombyx Mori Nucleopolyhedrovirus

Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus

2012 ◽  
Vol 40 (2) ◽  
pp. 865-873
Author(s):  
Yong Liu ◽  
Feng Yu ◽  
Huiling Wu ◽  
Qing Cao ◽  
Yu Wu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Open Reading Frame ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Reading Frame

Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production

2010 ◽  
Vol 151 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Zhong-Jian Guo ◽  
Li-Hua Qiu ◽  
Shi-Heng An ◽  
Qin Yao ◽  
Enoch Y. Park ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Virus Production ◽  
Open Reading Frame ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Reading Frame ◽  
Budded Virus

scholarly journals Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17

2012 ◽  
Vol 64 (6) ◽  
pp. 711-718 ◽  
Author(s):  
Hongxing Shen ◽  
Yang Zhou ◽  
Wen Zhang ◽  
Bin Nin ◽  
Hua Wang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

Characterization of Bombyx mori parvo-like virus non-structural protein NS1

Virus Genes ◽  
2009 ◽  
Vol 39 (3) ◽  
pp. 396-402 ◽  
Author(s):  
Guohui Li ◽  
Chen Sun ◽  
Junhong Zhang ◽  
Yuanqing He ◽  
Huiqing Chen ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Structural Protein

Characterization of an early gene orf122 from Bombyx mori nucleopolyhedrovirus

2008 ◽  
Vol 36 (3) ◽  
pp. 543-548 ◽  
Author(s):  
Jun-Qing Ge ◽  
Jin-Fang Zhao ◽  
Ya-Ming Shao ◽  
Cai-Hong Tian ◽  
Chuan-Xi Zhang
Keyword(s):  
Bombyx Mori ◽  
Early Gene ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus

BMB Reports ◽  
2006 ◽  
Vol 39 (6) ◽  
pp. 737-742 ◽  
Author(s):  
Meng-Fang Du ◽  
Xin-Ming Yin ◽  
Zhong-Jian Guo ◽  
Liang-Jun Zhu
Keyword(s):  
Bombyx Mori ◽  
Late Gene ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in budded virus production and nucleocapsid assembly

2013 ◽  
Vol 94 (7) ◽  
pp. 1669-1679 ◽  
Author(s):  
Xingwei Xiang ◽  
Yunwang Shen ◽  
Rui Yang ◽  
Lin Chen ◽  
Xiaolong Hu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Virus Production ◽  
Full Length ◽  
Tubular Structures ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Budded Virus ◽  
Conserved Gene ◽  
Fluorescence And Light Microscopy

Bombyx mori nucleopolyhedrovirus (BmNPV) BmP95 is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of BmP95 in virus infection in vitro, a BmP95 deletion virus (vBmP95-De) was generated by homologous recombination in Escherichia coli. Fluorescence and light microscopy and titration analysis indicated that the BmP95 deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of BmP95 did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the BmP95 deletion bacmid, indicating that deletion of BmP95 affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length BmP95 into the polyhedrin locus of the BmP95-knockout bacmid but not the N-terminal domain of BmP95. Together, these results showed that full-length BmP95 is essential for BV production and is required for nucleocapsid assembly.

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