scholarly journals Characterization of Bovine Adenovirus Type 3 E1 Proteins and Isolation of E1-Expressing Cell Lines

Virology ◽  
2002 ◽  
Vol 295 (1) ◽  
pp. 108-118 ◽  
Author(s):  
Alberto L. van Olphen ◽  
Suresh K. Tikoo ◽  
Suresh K. Mittal
Keyword(s):  
Cell Lines ◽  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Bovine Adenovirus Type 3 ◽  
Type 3

scholarly journals Replication-Defective Bovine Adenovirus Type 3 as an Expression Vector

1999 ◽  
Vol 73 (11) ◽  
pp. 9137-9144 ◽  
Author(s):  
P. Seshidhar Reddy ◽  
Neeraja Idamakanti ◽  
Yan Chen ◽  
Tyler Whale ◽  
Lorne A. Babiuk ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cell Lines ◽  
Expression Vector ◽  
Adenovirus Type ◽  
Somatic Gene Therapy ◽  
Bovine Adenovirus ◽  
Bovine Retina ◽  
Somatic Gene ◽  
Bovine Adenovirus Type 3 ◽  
Type 3

ABSTRACT Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus type 3 (BAV-3) was developed as an expression vector. Bovine cell lines designated VIDO R2 (HAV-5 E1A/B-transformed fetal bovine retina cell [FBRC] line) and 6.93.9 (Madin-Darby bovine kidney [MDBK] cell line expressing E1 proteins) were developed and found to complement the E1A deletion in BAV-3. Replication-defective BAV-3 with a 1.7-kb deletion removing most of the E1A and E3 regions was constructed. This virus could be grown in VIDO R2 or 6.93.9 cells but not in FBRC or MDBK cells. The results demonstrated that the E1 region of HAV-5 has the capacity to transform bovine retina cells and that the E1A region of HAV-5 can complement that of BAV-3. A replication-defective BAV-3 vector expressing bovine herpesvirus type 1 glycoprotein D from the E1A region was made. A similar replication-defective vector expressing the hemagglutinin-esterase gene of bovine coronavirus from the E3 region was isolated. Although these viruses grew less efficiently than the replication-competent recombinant BAV-3 (E3 deleted), they are suitable for detailed studies with animals to evaluate the safety, duration of foreign gene expression, and ability to induce immune responses. In addition, a replication-competent recombinant BAV-3 expressing green fluorescent protein was constructed and used to evaluate the host range of BAV-3 under cell culture conditions. The development of bovine E1A-complementing cell lines and the generation of replication-defective BAV-3 vectors is a major technical advancement for defining the use of BAV-3 as vector for vaccination against diseases of cattle and somatic gene therapy in humans.

scholarly journals Transcription Mapping and Characterization of 284R and 121R Proteins Produced from Early Region 3 of Bovine Adenovirus Type 3

Virology ◽  
1999 ◽  
Vol 256 (2) ◽  
pp. 351-359 ◽  
Author(s):  
Neeraja Idamakanti ◽  
P.Seshidhar Reddy ◽  
Lorne A. Babiuk ◽  
Suresh K. Tikoo
Keyword(s):  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Early Region ◽  
Bovine Adenovirus Type 3 ◽  
Type 3 ◽  
Early Region 3

scholarly journals Mutational Analysis of Early Region 4 of Bovine Adenovirus Type 3

Virology ◽  
2001 ◽  
Vol 290 (1) ◽  
pp. 153-163 ◽  
Author(s):  
Mohit K. Baxi ◽  
Jill Robertson ◽  
Lorne A. Babiuk ◽  
Suresh K. Tikoo
Keyword(s):  
Mutational Analysis ◽  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Early Region ◽  
Early Region 4 ◽  
Bovine Adenovirus Type 3 ◽  
Type 3

scholarly journals Development of a bovine adenovirus type 3-based expression vector

1995 ◽  
Vol 76 (1) ◽  
pp. 93-102 ◽  
Author(s):  
S. K. Mittal ◽  
L. Prevec ◽  
F. L. Graham ◽  
L. A. Babiuk
Keyword(s):  
Expression Vector ◽  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Bovine Adenovirus Type 3 ◽  
Type 3

scholarly journals Oncogenicity of Bovine Adenovirus Type 3 in Hamsters

Nature ◽  
1966 ◽  
Vol 211 (5044) ◽  
pp. 102-102 ◽  
Author(s):  
J. H. DARBYSHIRE
Keyword(s):  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Bovine Adenovirus Type 3 ◽  
Type 3

scholarly journals Cryo-EM structures of two bovine adenovirus type 3 intermediates

Virology ◽  
2014 ◽  
Vol 450-451 ◽  
pp. 174-181 ◽  
Author(s):  
Lingpeng Cheng ◽  
Xiaoxing Huang ◽  
Xiaomin Li ◽  
Wei Xiong ◽  
Wei Sun ◽  
...  
Keyword(s):  
Adenovirus Type ◽  
Bovine Adenovirus ◽  
Bovine Adenovirus Type 3 ◽  
Type 3
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