scholarly journals Characterisation of Sri Lankan Cassava Mosaic Virus and Indian Cassava Mosaic Virus: Evidence for Acquisition of a DNA B Component by a Monopartite Begomovirus

Virology ◽  
2002 ◽  
Vol 293 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Keith Saunders ◽  
Nazeera Salim ◽  
Vasant R. Mali ◽  
Varagur G. Malathi ◽  
Rob Briddon ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sri Lankan ◽  
Monopartite Begomovirus ◽  
Indian Cassava Mosaic Virus ◽  
Cassava Mosaic Virus

scholarly journals Emergence of a Latent Indian Cassava Mosaic Virus from Cassava Which Recovered from Infection by a Non-Persistent Sri Lankan Cassava Mosaic Virus

Viruses ◽  
2016 ◽  
Vol 8 (10) ◽  
pp. 264 ◽  
Author(s):  
Chockalingam Karthikeyan ◽  
Basavaprabhu Patil ◽  
Basanta Borah ◽  
Thulasi Resmi ◽  
Silvia Turco ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sri Lankan ◽  
Indian Cassava Mosaic Virus ◽  
Cassava Mosaic Virus

scholarly journals Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Saengsoon Charoenvilaisiri ◽  
Channarong Seepiban ◽  
Mallika Kumpoosiri ◽  
Sombat Rukpratanporn ◽  
Nuchnard Warin ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Mosaic Disease ◽  
Cassava Mosaic Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sri Lankan ◽  
Stem Cuttings ◽  
Field Samples ◽  
Cassava Stem ◽  
Cassava Mosaic Virus

Abstract Background Cassava mosaic disease (CMD) is one of the most devastating viral diseases for cassava production in Africa and Asia. Accurate yet affordable diagnostics are one of the fundamental tools supporting successful CMD management, especially in developing countries. This study aimed to develop an antibody-based immunoassay for the detection of Sri Lankan cassava mosaic virus (SLCMV), the only cassava mosaic begomovirus currently causing CMD outbreaks in Southeast Asia (SEA). Methods Monoclonal antibodies (MAbs) against the recombinant coat protein of SLCMV were generated using hybridoma technology. MAbs were characterized and used to develop a triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) for SLCMV detection in cassava leaves and stems. Assay specificity, sensitivity and efficiency for SLCMV detection was investigated and compared to those of a commercial ELISA test kit and PCR, the gold standard. Results A TAS-ELISA for SLCMV detection was successfully developed using the newly established MAb 29B3 and an in-house polyclonal antibody (PAb) against begomoviruses, PAb PK. The assay was able to detect SLCMV in leaves, green bark from cassava stem tips, and young leaf sprouts from stem cuttings of SLCMV-infected cassava plants without cross-reactivity to those derived from healthy cassava controls. Sensitivity comparison using serial dilutions of SLCMV-infected cassava sap extracts revealed that the assay was 256-fold more sensitive than a commercial TAS-ELISA kit and 64-fold less sensitive than PCR using previously published SLCMV-specific primers. In terms of DNA content, our assay demonstrated a limit of detection of 2.21 to 4.08 × 106 virus copies as determined by quantitative real-time PCR (qPCR). When applied to field samples (n = 490), the TAS-ELISA showed high accuracy (99.6%), specificity (100%), and sensitivity (98.2%) relative to the results obtained by the reference PCR. SLCMV infecting chaya (Cnidoscolus aconitifolius) and coral plant (Jatropha multifida) was also reported for the first time in SEA. Conclusions Our findings suggest that the TAS-ELISA for SLCMV detection developed in this study can serve as an attractive tool for efficient, inexpensive and high-throughput detection of SLCMV and can be applied to CMD screening of cassava stem cuttings, large-scale surveillance, and screening for resistance.

scholarly journals First Report of Sri Lankan cassava mosaic virus Infecting Cassava in Cambodia

Plant Disease ◽  
2016 ◽  
Vol 100 (5) ◽  
pp. 1029-1029 ◽  
Author(s):  
H. L. Wang ◽  
X. Y. Cui ◽  
X. W. Wang ◽  
S. S. Liu ◽  
Z. H. Zhang ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Sri Lankan ◽  
First Report ◽  
Cassava Mosaic Virus

scholarly journals Resistance to Sri Lankan Cassava Mosaic Virus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0120551 ◽  
Author(s):  
Valentine Otang Ntui ◽  
Kynet Kong ◽  
Raham Sher Khan ◽  
Tomoko Igawa ◽  
Gnanaguru Janaky Janavi ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Genetically Engineered ◽  
Sri Lankan ◽  
Cassava Mosaic Virus
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