scholarly journals Amphotropic Murine Leukemia Virus Replication in Human Mammary Epithelial Cells and the Formation of Cytomegalovirus–Promoter Recombinants

Virology ◽  
2001 ◽  
Vol 291 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Frank U. Reuss ◽  
Ricarda Heber ◽  
Alexander Ploss ◽  
Bianca Berdel
Keyword(s):  
Epithelial Cells ◽  
Virus Replication ◽  
Murine Leukemia Virus ◽  
Mammary Epithelial Cells ◽  
Leukemia Virus ◽  
Mammary Epithelial ◽  
Cytomegalovirus Promoter ◽  
Amphotropic Murine Leukemia Virus ◽  
Human Mammary Epithelial ◽  
Murine Leukemia

scholarly journals Viral oncogenes accelerate conversion to immortality of cultured conditionally immortal human mammary epithelial cells

Oncogene ◽  
1999 ◽  
Vol 18 (13) ◽  
pp. 2169-2180 ◽  
Author(s):  
James Garbe ◽  
Michelle Wong ◽  
Don Wigington ◽  
Paul Yaswen ◽  
Martha R Stampfer
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial Cells ◽  
Viral Oncogenes ◽  
Human Mammary Epithelial

scholarly journals The ETS Transcription Factor ESE-1 Transforms MCF-12A Human Mammary Epithelial Cells via a Novel Cytoplasmic Mechanism

2004 ◽  
Vol 24 (12) ◽  
pp. 5548-5564 ◽  
Author(s):  
Jason D. Prescott ◽  
Karen S. N. Koto ◽  
Meenakshi Singh ◽  
Arthur Gutierrez-Hartmann
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Epithelial Cells ◽  
Nuclear Localization ◽  
Human Breast Cancer ◽  
Mammary Epithelial Cells ◽  
Cell Transformation ◽  
Mammary Epithelial ◽  
Human Breast ◽  
Human Mammary Epithelial

ABSTRACT Several different transcription factors, including estrogen receptor, progesterone receptor, and ETS family members, have been implicated in human breast cancer, indicating that transcription factor-induced alterations in gene expression underlie mammary cell transformation. ESE-1 is an epithelium-specific ETS transcription factor that contains two distinguishing domains, a serine- and aspartic acid-rich (SAR) domain and an AT hook domain. ESE-1 is abundantly expressed in human breast cancer and trans-activates epithelium-specific gene promoters in transient transfection assays. While it has been presumed that ETS factors transform mammary epithelial cells via their nuclear transcriptional functions, here we show (i) that ESE-1 protein is cytoplasmic in human breast cancer cells; (ii) that stably expressed green fluorescent protein-ESE-1 transforms MCF-12A human mammary epithelial cells; and (iii) that the ESE-1 SAR domain, acting in the cytoplasm, is necessary and sufficient to mediate this transformation. Deletion of transcriptional regulatory or nuclear localization domains does not impair ESE-1-mediated transformation, whereas fusing the simian virus 40 T-antigen nuclear localization signal to various ESE-1 constructs, including the SAR domain alone, inhibits their transforming capacity. Finally, we show that the nuclear localization of ESE-1 protein induces apoptosis in nontransformed mammary epithelial cells via a transcription-dependent mechanism. Together, our studies reveal two distinct ESE-1 functions, apoptosis and transformation, where the ESE-1 transcription activation domain contributes to apoptosis and the SAR domain mediates transformation via a novel nonnuclear, nontranscriptional mechanism. These studies not only describe a unique ETS factor transformation mechanism but also establish a new paradigm for cell transformation in general.

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