scholarly journals Molecular Cloning and Biological Characterization of Full-Length HIV-1 Subtype C from Botswana

Virology ◽  
2000 ◽  
Vol 278 (2) ◽  
pp. 390-399 ◽  
Author(s):  
Thumbi Ndung'u ◽  
Boris Renjifo ◽  
Vladimir A. Novitsky ◽  
Mary Fran McLane ◽  
Sarah Gaolekwe ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Full Length ◽  
Biological Characterization ◽  
Subtype C ◽  
Hiv 1

Molecular Cloning, Expression, and Biological Characterization of an HTLV-II Envelope Glycoprotein: HIV-1 Expression Is Permissive for HTLV-II-Induced Cell Fusion

1993 ◽  
Vol 9 (9) ◽  
pp. 849-860 ◽  
Author(s):  
BIN WANG ◽  
MICHAEL G. AGADJANYAN ◽  
VASANTHA SRIKANTAN ◽  
KENNETH E. UGEN ◽  
WILLIAM HALL ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cell Fusion ◽  
Envelope Glycoprotein ◽  
Biological Characterization ◽  
Hiv 1

scholarly journals Identification, Molecular Cloning, and Analysis of Full-Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Mark B. Stoddard ◽  
Hui Li ◽  
Shuyi Wang ◽  
Mohsan Saeed ◽  
Linda Andrus ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Molecular Cloning ◽  
Acute Phase ◽  
Vaccine Development ◽  
Virus Transmission ◽  
Full Length ◽  
Single Genome ◽  
Hiv 1

ABSTRACTHepatitis C virus (HCV) infection is characterized by persistent replication of a complex mixture of viruses termed a “quasispecies.” Transmission is generally associated with a stringent population bottleneck characterized by infection by limited numbers of “transmitted/founder” (T/F) viruses. Characterization of T/F genomes of human immunodeficiency virus type 1 (HIV-1) has been integral to studies of transmission, immunopathogenesis, and vaccine development. Here, we describe the identification of complete T/F genomes of HCV by single-genome sequencing of plasma viral RNA from acutely infected subjects. A total of 2,739 single-genome-derived amplicons comprising 10,966,507 bp from 18 acute-phase and 11 chronically infected subjects were analyzed. Acute-phase sequences diversified essentially randomly, except for the poly(U/UC) tract, which was subject to polymerase slippage. Fourteen acute-phase subjects were productively infected by more than one genetically distinct virus, permitting assessment of recombination between replicating genomes. No evidence of recombination was found among 1,589 sequences analyzed. Envelope sequences of T/F genomes lacked transmission signatures that could distinguish them from chronic infection viruses. Among chronically infected subjects, higher nucleotide substitution rates were observed in the poly(U/UC) tract than in envelope hypervariable region 1. Fourteen full-length molecular clones with variable poly(U/UC) sequences corresponding to seven genotype 1a, 1b, 3a, and 4a T/F viruses were generated. Like most unadapted HCV clones, T/F genomes did not replicate efficiently in Huh 7.5 cells, indicating that additional cellular factors or viral adaptations are necessary forin vitroreplication. Full-length T/F HCV genomes and their progeny provide unique insights into virus transmission, virus evolution, and virus-host interactions associated with immunopathogenesis.IMPORTANCEHepatitis C virus (HCV) infects 2% to 3% of the world's population and exhibits extraordinary genetic diversity. This diversity is mirrored by HIV-1, where characterization of transmitted/founder (T/F) genomes has been instrumental in studies of virus transmission, immunopathogenesis, and vaccine development. Here, we show that despite major differences in genome organization, replication strategy, and natural history, HCV (like HIV-1) diversifies essentially randomly early in infection, and as a consequence, sequences of actual T/F viruses can be identified. This allowed us to capture by molecular cloning the full-length HCV genomes that are responsible for infecting the first hepatocytes and eliciting the initial immune responses, weeks before these events could be directly analyzed in human subjects. These findings represent an enabling experimental strategy, not only for HCV and HIV-1 research, but also for other RNA viruses of medical importance, including West Nile, chikungunya, dengue, Venezuelan encephalitis, and Ebola viruses.

scholarly journals Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita

2014 ◽  
Vol 13 (4) ◽  
pp. 10994-11003 ◽  
Author(s):  
S.P. Ling ◽  
S.S. Su ◽  
H.M. Zhang ◽  
X.S. Zhang ◽  
X.Y. Liu ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Full Length ◽  
Matricaria Recutita

Expression, purification and characterization of a full-length recombinant HIV-1 Vpu from inclusion bodies

2016 ◽  
Vol 128 ◽  
pp. 109-114 ◽  
Author(s):  
Zikhona Njengele ◽  
Ronel Kleynhans ◽  
Yasien Sayed ◽  
Salerwe Mosebi
Keyword(s):  
Inclusion Bodies ◽  
Full Length ◽  
Purification And Characterization ◽  
Hiv 1

scholarly journals Construction and Characterization of Highly Infectious Full-Length Molecular Clones of a HIV-1 CRF07_BC Isolate from Xinjiang, China

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79177 ◽  
Author(s):  
Zheng Wang ◽  
Kunxue Hong ◽  
Jing Zhang ◽  
Lei Zhang ◽  
Dan Li ◽  
...  
Keyword(s):  
Full Length ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document