scholarly journals Mutational Analysis of Two Structural Genes of the Temperate Lactococcal Bacteriophage TP901-1 Involved in Tail Length Determination and Baseplate Assembly

Virology ◽  
2000 ◽  
Vol 276 (2) ◽  
pp. 315-328 ◽  
Author(s):  
Margit Pedersen ◽  
Solvej Østergaard ◽  
José Bresciani ◽  
Finn K. Vogensen
Keyword(s):  
Mutational Analysis ◽  
Tail Length ◽  
Structural Genes ◽  
Length Determination

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905 ◽  
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals PASP — a whole-transcriptome poly(A) tail length determination assay for the Illumina platform

2016 ◽  
Author(s):  
Botond Sipos ◽  
Adrian M Stütz ◽  
Greg Slodkowicz ◽  
Tim Massingham ◽  
Jan Korbel ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Tail Length ◽  
Translational Efficiency ◽  
Illumina Platform ◽  
Regulatory Pathways ◽  
Length Determination ◽  
High Consistency ◽  
Post Transcriptional Regulation ◽  
Whole Transcriptome ◽  
Cdna Fragments

AbstractThe poly(A) tail, co-transcriptionally added to most eukaryotic RNAs, plays an important role in post-transcriptional regulation through modulating mRNA stability and translational efficiency. The length of the poly(A) tail is dynamic, decreasing or increasing in response to various stimuli through the action of enzymatic complexes, and changes in tail length are exploited in regulatory pathways implicated in various biological processes.To date, assessment of poly(A) tail length has mostly relied on protocols targeting only a few transcripts. We present PASP (‘poly(A) tailsequencingprotocol’), a whole-transcriptome approach to measure tail lengths — including a computational pipeline implementing all necessary analyses. PASP uses direct Illumina sequencing of cDNA fragments obtained through G-tailing of poly(A)-selected mRNA followed by fragmentation and reverse transcription.Analysis of reads corresponding to spike-in poly(A) tracts of known length indicated that mean tail lengths can be confidently measured, given sufficient coverage. We further explored the utility of our approach by comparing tail lengths estimated from wild type and Δccr4-1/pan2mutant yeasts. The yeast whole-transcriptome tail length distributions showed high consistency between biological replicates, and the expected upward shift in tail lengths in the mutant samples was detected. This suggests that PASP is suitable for the assessment of global polyadenylation status in yeast.The correlation of per-transcript mean tail lengths between biological and technical replicates was low (higher between mutant samples). Both, however, reached high values after filtering for transcripts with greater coverage. We also compare our results with those of other methods. We identify a number of improvements that could be used in future PASP experiments and, based on our results, believe that direct sequencing of poly(A) tails can become the method of choice for studying polyadenylation using the Illumina platform

Tail Length Determination in Bacteriophage T4

Virology ◽  
1994 ◽  
Vol 199 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Natalia K. Abuladze ◽  
Mari Gingery ◽  
Jerry Tsai ◽  
Frederick A. Eiserling
Keyword(s):  
Bacteriophage T4 ◽  
Tail Length ◽  
Length Determination

scholarly journals Nucleotide sequences and mutational analysis of the structural genes for nitrogenase 2 of Azotobacter vinelandii.

1990 ◽  
Vol 172 (6) ◽  
pp. 3400-3408 ◽  
Author(s):  
R D Joerger ◽  
T M Loveless ◽  
R N Pau ◽  
L A Mitchenall ◽  
B H Simon ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Azotobacter Vinelandii ◽  
Nucleotide Sequences ◽  
Structural Genes
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