scholarly journals Mutational Analysis of the Bovine Respiratory Syncytial Virus Nucleocapsid Protein Using a Minigenome System: Mutations That Affect Encapsidation, RNA Synthesis, and Interaction with the Phosphoprotein

Virology ◽  
2000 ◽  
Vol 270 (1) ◽  
pp. 215-228 ◽  
Author(s):  
Sunil K. Khattar ◽  
Abdul S. Yunus ◽  
Peter L. Collins ◽  
Siba K. Samal
Keyword(s):  
Respiratory Syncytial Virus ◽  
Nucleocapsid Protein ◽  
Rna Synthesis ◽  
Mutational Analysis ◽  
Bovine Respiratory Syncytial Virus ◽  
Syncytial Virus ◽  
Minigenome System

scholarly journals Mapping the domains on the phosphoprotein of bovine respiratory syncytial virus required for N–P and P–L interactions using a minigenome system

2001 ◽  
Vol 82 (4) ◽  
pp. 775-779 ◽  
Author(s):  
Sunil K. Khattar ◽  
Abdul S. Yunus ◽  
Siba K. Samal
Keyword(s):  
Amino Acids ◽  
Respiratory Syncytial Virus ◽  
Binding Sites ◽  
Deletion Mutant ◽  
Bovine Respiratory Syncytial Virus ◽  
Binding Domains ◽  
P Protein ◽  
L Proteins ◽  
Syncytial Virus ◽  
Minigenome System

The interaction of bovine respiratory syncytial virus (BRSV) phosphoprotein (P) with nucleocapsid (N) and large polymerase (L) proteins was investigated using an intracellular BRSV–CAT minigenome replication system. Coimmunoprecipitation assays using P-specific antiserum revealed that the P protein can form complexes with N and L proteins. Deletion mutant analysis of the P protein was performed to identify the regions of P protein that interact with N and L proteins. The results indicate that two independent N-binding sites exist on the P protein: an internal region of 161–180 amino acids and a C-terminal region of 221–241 amino acids. The L-binding site was mapped to a region of P protein encompassing amino acids 121–160. The data suggest that N and L protein binding domains on the P protein do not overlap.

Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein

2006 ◽  
Vol 116 (1-2) ◽  
pp. 30-37 ◽  
Author(s):  
Fernando Rosado Spilki ◽  
Renata Servan Almeida ◽  
Helena Gallichio Domingues ◽  
Regina Celia Freitas D’Arce ◽  
Helena Lage Ferreira ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Homology Modeling ◽  
Phylogenetic Relationships ◽  
Bovine Respiratory Syncytial Virus ◽  
Syncytial Virus ◽  
Molecular Homology ◽  
Virus Isolates
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