scholarly journals Accessory Genes in thedarAOperon of Bacteriophage P1 Affect Antirestriction Function, Generalized Transduction, Head Morphogenesis, and Host Cell Lysis

Virology ◽  
1998 ◽  
Vol 251 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Shigeru Iida ◽  
Rosemarie Hiestand-Nauer ◽  
Heinrich Sandmeier ◽  
Hansjörg Lehnherr ◽  
Werner Arber
Keyword(s):  
Host Cell ◽  
Cell Lysis ◽  
Bacteriophage P1 ◽  
Accessory Genes ◽  
Generalized Transduction

scholarly journals RECIPIENT GENE DUPLICATION DURING GENERALIZED TRANSDUCTION

Genetics ◽  
1974 ◽  
Vol 78 (3) ◽  
pp. 809-822
Author(s):  
M Stodolsky
Keyword(s):  
Gene Duplication ◽  
Crossing Over ◽  
Chromosome Fragment ◽  
Bacteriophage P1 ◽  
Recombinant Formation ◽  
Generalized Transduction

ABSTRACT An Hfrl3 ΔProA-lac  deletion recipient, -ΔproA-lac-F-purE+-, has been utilized in a study of the origins of duplications formed during chromosome fragment integration. Among the Pro-Lac+ transductants, some have duplications spanning the F locus. These transductants are, or segregate, strains with F' episomes carrying genes of the duplication. Some of the duplications include purE+, a gene which is not coinherited with lac+ during bacteriophage P1- mediated transduction. Thus recipient genes have been duplicated during recombinant formation. Crossing-over models including replication steps provide a basis for explaining the duplication process.

scholarly journals Genome-wide identification of genetic requirements ofPseudomonas aeruginosaPAO1 for rat cardiomyocyte (H9C2) infection by insertion sequencing

2021 ◽  
Author(s):  
Jothi Ranjani ◽  
Ramamoorthy Sivakumar ◽  
Paramasamy Gunasekaran ◽  
Jeyaprakash Rajendhran
Keyword(s):  
Host Cell ◽  
Drug Targets ◽  
Infectious Agent ◽  
Cell Lysis ◽  
Growth Media ◽  
List Type ◽  
H9c2 Cells ◽  
Secretion Systems ◽  
Chronic Infections ◽  
Adhesion And Invasion

AbstractPseudomonas aeruginosais the major infectious agent among Gram-negative bacteria which causes both acute and chronic infections without any tissue specificity. Infections due toP. aeruginosaare hard to treat, as it entails various strategies like virulence factors synthesis, drug efflux systems & resistance and protein secretion systems during pathogenesis. Despite extensive research inPseudomonaspathogenesis, novel drug targets and potential therapeutic strategies are inevitable. In this study, we investigated the genetic requirements ofP.aeruginosaPAO1 for rat cardiomyocyte (H9C2) infection by insertion sequencing (INSeq). A mutant library comprising ~70,000 mutants of PAO1 was generated and the differentiated form of H9C2 cells (d-H9C2) was infected with the library. The infected d-H9C2 cells were maintained with antibiotic-protection and without any antibiotics in the growth media for 24 h. Subsequently, DNA library for INSeq was prepared, sequenced and fitness analysis was performed. A-One hundred and thirteen mutants were negatively selected in the infection condition with antibiotic-protection, whereas 143 mutants were negatively selected in antibiotic-free condition. Surprisingly, a higher number of mutants showed enriched fitness than the mutants of reduced fitness during the infection. We demonstrated that the genes associated with flagella and T3SS are important for adhesion and invasion of cardiomyocytes, while pili and proteases are conditionally essential during host cell lysis.Take away✓Fitness ofP.aeruginosamutants were analyzed during cardiomyocyte infection✓Genes involve amino acid transport & metabolism and signal transduction are important during intracellular lifestyle✓OMVs play a crucial role during infection and pathogenesis✓Flagella and T3SS are conditionally essential for adhesion and invasion, whereas pili and proteases are conditionally essential during host cell lysis

scholarly journals Lysis Genes of the Bacillus subtilisDefective Prophage PBSX

1998 ◽  
Vol 180 (8) ◽  
pp. 2110-2117 ◽  
Author(s):  
Susanne Krogh ◽  
Steen T. Jørgensen ◽  
Kevin M. Devine
Keyword(s):  
Functional Analysis ◽  
Host Cell ◽  
Expression System ◽  
Cell Lysis ◽  
Lethal Gene ◽  
Gram Negative Bacteria ◽  
Gene Products ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Exported Protein

ABSTRACT Four genes identified within the late operon of PBSX show characteristics expected of a host cell lysis system; they arexepA, encoding an exported protein; xhlA, encoding a putative membrane-associated protein; xhlB, encoding a putative holin; and xlyA, encoding a putative endolysin. In this work, we have assessed the contribution of each gene to host cell lysis by expressing the four genes in different combinations under the control of their natural promoter located on the chromosome of Bacillus subtilis 168. The results show thatxepA is unlikely to be involved in host cell lysis. Expression of both xhlA and xhlB is necessary to effect host cell lysis of B. subtilis. Expression ofxhlB (encoding the putative holin) together withxlyA (encoding the endolysin) cannot effect cell lysis, indicating that the PBSX lysis system differs from those identified in the phages of gram-negative bacteria. Since host cell lysis can be achieved when xlyA is inactivated, it is probable that PBSX encodes a second endolysin activity which also uses XhlA and XhlB for export from the cell. The chromosome-based expression system developed in this study to investigate the functions of the PBSX lysis genes should be a valuable tool for the analysis of other host cell lysis systems and for expression and functional analysis of other lethal gene products in gram-positive bacteria.

Excess haemoglobin digestion by malaria parasites: a strategy to prevent premature host cell lysis

2004 ◽  
Vol 32 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Virgilio L Lew ◽  
Lynn Macdonald ◽  
Hagai Ginsburg ◽  
Miriam Krugliak ◽  
Teresa Tiffert
Keyword(s):  
Host Cell ◽  
Cell Lysis ◽  
Malaria Parasites

Mutation in bacteriophage T3 affecting host cell lysis

Virology ◽  
1978 ◽  
Vol 89 (1) ◽  
pp. 327-329 ◽  
Author(s):  
Jun-Ichi Miyazaki ◽  
Yeikou Ryo ◽  
Hisao Fujisawa ◽  
Teiichi Minagawa
Keyword(s):  
Host Cell ◽  
Cell Lysis

Transduction of Escherichia coli in soil

1988 ◽  
Vol 34 (2) ◽  
pp. 190-193 ◽  
Author(s):  
James J. Germida ◽  
George G. Khachatourians
Keyword(s):  
Escherichia Coli ◽  
Silty Clay ◽  
Bacterial Populations ◽  
Bacteriophage P1 ◽  
E Coli ◽  
Loam Soil ◽  
K 12 ◽  
Generalized Transduction ◽  
Potential Use

Bacteriophage P1-mediated generalized transduction of Escherichia coli K-12 was assessed in nonsterile soil. Auxotrophic recipient cells (thr−leu−thi−rpsL) were incubated in a sandy and a silty clay loam soil, and the transducing phage lysates from prototrophic strains carrying transposon 10 (Tn10) in either purE or aroL regions were added. At intervals, the bacterial populations derived from the soils were plated on selective-differential media to enumerate prototrophic (thr+, leu+, or Tcr) transductants. Of 100 bacterial isolates obtained on the selective-differential media, 58 (14 thr+; 11, leu+; 33 Tcr) were confirmed E. coli transductants. The frequency of transduction in soil was ca. 10−6. These data demonstrate the potential use of bacteriophage P1 to genetically manipulate E. coli in situ.

Mutations in bacteriophage lambda affecting host cell lysis

Virology ◽  
1967 ◽  
Vol 32 (4) ◽  
pp. 553-569 ◽  
Author(s):  
A.W. Harris ◽  
D.W.A. Mount ◽  
C.R. Fuerst ◽  
L. Siminovitch
Keyword(s):  
Host Cell ◽  
Bacteriophage Lambda ◽  
Cell Lysis

scholarly journals Phi X174 E complements lambda S and R dysfunction for host cell lysis.

1993 ◽  
Vol 175 (12) ◽  
pp. 3909-3912 ◽  
Author(s):  
W D Roof ◽  
R Young
Keyword(s):  
Host Cell ◽  
Cell Lysis

Chlamydial infections

2020 ◽  
pp. 1278-1295
Author(s):  
Patrick Horner ◽  
David Mabey ◽  
David Taylor-Robinson ◽  
Magnus Unemo
Keyword(s):  
Chlamydia Trachomatis ◽  
Tract Infection ◽  
16S Rrna ◽  
Host Cell ◽  
Pathogenic Bacteria ◽  
Cell Lysis ◽  
Lymphogranuloma Venereum ◽  
23S Rrna ◽  
Gram Negative ◽  
Rrna Sequences

Chlamydiae are pathogenic bacteria that likely evolved from host-independent, Gram-negative ancestors. Chlamydiae depend on a eukaryotic host cell for their replication which takes place in an inclusion inside the host cell, and for their dispersal, cell lysis, or extrusion subsequently occurs. Although the phylum Chlamydiae (order Chlamydiales) was originally thought to only contain one family, the Chlamydiaceae, a total of nine families are now recognized. The genus Chlamydia remains the most widely studied. The species Chlamydia trachomatis was proposed some decades ago on the basis of 16S rRNA and 23S rRNA sequences, to belong to the genus Chlamydia together with C. muridarum and C. suis. This chapter primarily focuses on the species C. trachomatis, which causes disease of ocular trachoma (serovars A–C), oculo-anogenital tract infection (serovars D–K) and lymphogranuloma venereum (serovars L1–L3). However, infections caused by C. pneumoniae and C. psittaci are also discussed.

New mutations in the S cistron of bacteriophage lambda affecting host cell lysis

Virology ◽  
1969 ◽  
Vol 38 (1) ◽  
pp. 200-202 ◽  
Author(s):  
Allan R. Goldberg ◽  
Martha Howe
Keyword(s):  
Host Cell ◽  
Bacteriophage Lambda ◽  
Cell Lysis ◽  
New Mutations
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