scholarly journals Combined DNA Immunization with the Glycoprotein Gene of Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus Induces Double-Specific Protective Immunity and Nonspecific Response in Rainbow Trout

Virology ◽  
1998 ◽  
Vol 249 (2) ◽  
pp. 297-306 ◽  
Author(s):  
Pierre Boudinot ◽  
Mar Blanco ◽  
Pierre de Kinkelin ◽  
Abdenour Benmansour
Keyword(s):  
Rainbow Trout ◽  
Protective Immunity ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Dna Immunization ◽  
Necrosis Virus ◽  
Glycoprotein Gene ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Hematopoietic Necrosis

Development of a Liquid Chip Technique to Simultaneously Detect Spring Viremia of Carp Virus, Infectious Hematopoietic Necrosis Virus, and Viral Hemorrhagic Septicemia of Salmonids

2017 ◽  
Vol 100 (1) ◽  
pp. 159-164 ◽  
Author(s):  
Guixiang Tong ◽  
Xinxian Wei ◽  
Weili Yin ◽  
Xiaoguang Liao ◽  
Kai Yang ◽  
...  
Keyword(s):  
Reaction System ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Infectious Pancreatic Necrosis Virus ◽  
Sea Bream ◽  
N Gene ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Nervous Necrosis Virus ◽  
Necrosis Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Hematopoietic Necrosis

Abstract A liquid chip technique was developed to detect spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) of salmonids simultaneously. Sequencesof the G gene of SVCV, N gene of IHNV, and G gene of VHSV were used to design SVCV-, IHNV-, and VHSV-specific primers, which were labeled with biotin and subjected to amination modification. They were then coupled with fluorescence-coded microspheres and used for hybridization with reverse-transcription PCR products of SVCV, IHNV, and VHSV. A BD FACSArray was used to detect fluorescencesignal in the reaction system. This assay system had a high sensitivity to SVCV, VHSV, and IHNV, with LODs of 10, 10, and 100 pg/μL, respectively. Moreover, the assay was specific for the detection of SVCV, IHNV, and VHSV and was not susceptible to cross-detection of other viruses, including pike fry rhabdovirus, hirame rhabdovirus, infectious pancreatic necrosis virus, viral nervous necrosis virus, yellowtail ascites virus, grass carp reovirus, red sea bream iridovirus, and koi herpesvirus. The liquid chip assay technique established inthis study provides a novel, convenient, and rapid approach for the detection of SVCV, IHNV, and VHSV.

scholarly journals Limited Interference at the Early Stage of Infection between Two Recombinant Novirhabdoviruses: Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus

2010 ◽  
Vol 84 (19) ◽  
pp. 10038-10050 ◽  
Author(s):  
Stéphane Biacchesi ◽  
Annie Lamoureux ◽  
Emilie Mérour ◽  
Julie Bernard ◽  
Michel Brémont
Keyword(s):  
Early Stage ◽  
Cell Monolayer ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Antiviral Protein ◽  
Necrosis Virus ◽  
Interference Phenomenon ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Hematopoietic Necrosis

ABSTRACT The genome sequence of a hypervirulent novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) French strain 23-75, was determined. Compared to the genome of the prototype Fil3 strain, a number of substitutions, deletions, and insertions were observed. Following the establishment of a plasmid-based minigenome replication assay, recombinant VHSV (rVHSV) was successfully recovered. rVHSV exhibits wild-type-like growth properties in vitro as well as in vivo in rainbow trout. The dispensable role of NV for the novirhabdovirus replication was confirmed by generating rVHSV-ΔNV, in which the NV gene was deleted. This deletion mutant was shown to be as debilitated as that previously described for infectious hematopoietic necrosis virus (IHNV), a distantly related novirhabdovirus (S. Biacchesi, M. I. Thoulouze, M. Bearzotti, Y. X. Yu, and M. Bremont, J. Virol. 74:11247-11253, 2000). Recombinant VHSV and IHNV expressing tdTomato and GFPmax reporter genes, respectively, were generated, demonstrating the potential of these rhabdoviruses to serve as viral vectors. Interestingly, rIHNV-GFPmax could be recovered using the replicative complex proteins of either virus, whereas rVHSV-Tomato could be recovered only by using its own replicative complex, reflecting that the genome signal sequences of VHSV are relatively distant from those of IHNV and do not allow their cross-recognition. Moreover, the use of heterologous protein combinations underlined the importance of strong protein-protein interactions for the formation of a functional ribonucleoprotein complex. The rIHNV-GFPmax and rVHSV-Tomato viruses were used to simultaneously coinfect cell monolayers. It was observed that up to 74% of the cell monolayer was coinfected by both viruses, demonstrating that a limited interference phenomenon exists during the early stage of primary infection, and it was not mediated by a cellular antiviral protein or by some of the viral proteins.

Sign in / Sign up

Export Citation Format

Share Document