scholarly journals Assay for Movement of Lepidopteran Transposon IFP2 in Insect Cells Using a Baculovirus Genome as a Target DNA

Virology ◽  
1995 ◽  
Vol 211 (2) ◽  
pp. 397-407 ◽  
Author(s):  
Malcolm J. Fraser ◽  
Lynne Cary ◽  
Kitima Boonvisudhi ◽  
Hwei-Gene Heidi Wang
Keyword(s):  
Insect Cells ◽  
Baculovirus Genome ◽  
Target Dna

Evaluation of the nucleopolyhedrovirus of Anticarsia gemmatalis as a vector for gene therapy in mammals

2020 ◽  
Vol 20 ◽  
Author(s):  
Cintia N. Parsza ◽  
Diego L. Mengual Gómez ◽  
Jorge Alejandro Simonin ◽  
Mariano Nicolás Belaich ◽  
Pablo Daniel Ghiringhelli
Keyword(s):  
Gene Therapy ◽  
Mammalian Cells ◽  
Insect Cells ◽  
Autographa Californica ◽  
Anticarsia Gemmatalis ◽  
Agricultural Pests ◽  
Mammalian Cell Lines ◽  
Delivery Vector ◽  
Wide Range ◽  
Insect Pathogens

Background: Baculoviruses are insect pathogens with important biotechnological applications that transcend their use as biological controllers of agricultural pests. One species, Autographa californica multiple nucleopolhyedrovirus (AcMNPV) has been extensively exploited as a molecular platform to produce recombinant proteins and as a delivery vector for genes in mammals, because it can transduce a wide range of mammalian cells and tissues without replicating or producing progeny. Objective/Method: To investigate if the budded virions of Anticarsia gemmatalis multiple nucleopolhyedrovirus (AgMNPV) species has the same ability, the viral genome was modified by homologous recombination into susceptible insect cells to integrate reporter genes and then it was evaluated on mammalian cell lines in comparative form with respect to equivalent viruses derived from AcMNPV. Besides, the replicative capacity of AgMNPV´s virions in mammals was determined. Results: The experiments carried out showed that the recombinant variant of AgMNPV transduces and support the expression of delivered genes but not replicates in mammalian cells. Conclusion: Consequently, this insect pathogen is proposed as an alternative of non-infectious viruses in humans to explore new approaches in gene therapy and other applications based on the use of mammalian cells.

Beyond DNA-targeting in Cancer Chemotherapy. Emerging Frontiers- A Review

2020 ◽  
Vol 20 ◽  
Author(s):  
S.N Mbugua ◽  
L.W Njenga ◽  
R.A Odhiambo ◽  
S.O Wandiga ◽  
M.O Onani
Keyword(s):  
Superparamagnetic Iron Oxide ◽  
Active Centre ◽  
Dna Targeting ◽  
Advantages And Disadvantages ◽  
Cancer Pathogenesis ◽  
Ongoing Work ◽  
Target Dna ◽  
Anti Cancer ◽  
New Research ◽  
Innovative Drugs

: Modern anticancer drugs target DNA specifically on rapid division of malignant cells. One downside of this approach is that they also target other rapidly dividing healthy cells such as those involved in hair growth leading to serious toxic side effects and hair loss. Therefore, it would be better to develop novel agents that address cellular signalling mechanisms unique to cancerous cells, and new research is now focussing on such approaches. Although the classical chemotherapy area involving DNA as the set target continues to produce important findings, nevertheless, a distinctly discernible emerging trend is the divergence from the cisplatin operation model that uses the metal as the primary active centre of the drug. Many successful anti-cancer drugs present are associated with elevated toxicity levels. Cancers also develop immunity against most therapies and the area of cancer research can therefore be seen as an area with a high unaddressed need. Hence, ongoing work into cancer pathogenesis is important to create accurate preclinical tests which can contribute to the development of innovative drugs to manage and treat cancer. Some of the emergent frontiers utilizing different approaches include nanoparticles delivery, use of quantum dots, metal complexes, tumour ablation, magnetic hypothermia and hyperthermia by use of Superparamagnetic Iron oxide Nanostructures, pathomics and radiomics, laser surgery and exosomes. This review summarizes these new approaches in good detail giving critical views with necessary comparisons. It also delves into what they carry for the future including their advantages and disadvantages.

Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.

1990 ◽  
Vol 64 (10) ◽  
pp. 4799-4807 ◽  
Author(s):  
A Höss ◽  
I Moarefi ◽  
K H Scheidtmann ◽  
L J Cisek ◽  
J L Corden ◽  
...  
Keyword(s):  
Insect Cells ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Baculovirus Vector ◽  
Phosphorylation Pattern

Production of ethanol by cultured insect cells

1995 ◽  
Vol 31 (11) ◽  
pp. 876-879 ◽  
Author(s):  
Masakazu Takahashi ◽  
Yoshiaki Kono ◽  
Kazuhiro Matsushita ◽  
Jun Mitsuhashi
Keyword(s):  
Insect Cells

scholarly journals A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-trisphosphate Receptors

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1031
Author(s):  
Umut Toprak ◽  
Cansu Doğan ◽  
Dwayne Hegedus
Keyword(s):  
Pest Control ◽  
Structural Organization ◽  
Insect Cells ◽  
Expression Patterns ◽  
Regulatory Mechanisms ◽  
Comparative Approach ◽  
Insect Development ◽  
Current Review ◽  
Inositol 1,4,5 Trisphosphate ◽  
Target Sites

Calcium (Ca2+) homeostasis is vital for insect development and metabolism, and the endoplasmic reticulum (ER) is a major intracellular reservoir for Ca2+. The inositol 1,4,5- triphosphate receptor (IP3R) and ryanodine receptor (RyR) are large homotetrameric channels associated with the ER and serve as two major actors in ER-derived Ca2+ supply. Most of the knowledge on these receptors derives from mammalian systems that possess three genes for each receptor. These studies have inspired work on synonymous receptors in insects, which encode a single IP3R and RyR. In the current review, we focus on a fundamental, common question: “why do insect cells possess two Ca2+ channel receptors in the ER?”. Through a comparative approach, this review covers the discovery of RyRs and IP3Rs, examines their structures/functions, the pathways that they interact with, and their potential as target sites in pest control. Although insects RyRs and IP3Rs share structural similarities, they are phylogenetically distinct, have their own structural organization, regulatory mechanisms, and expression patterns, which explains their functional distinction. Nevertheless, both have great potential as target sites in pest control, with RyRs currently being targeted by commercial insecticide, the diamides.

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