Conjugative Transposition

2001 ◽  
pp. 455-456
Author(s):  
G. Churchward
Keyword(s):  
Conjugative Transposition

scholarly journals Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase.

1991 ◽  
Vol 173 (14) ◽  
pp. 4347-4352 ◽  
Author(s):  
M J Storrs ◽  
C Poyart-Salmeron ◽  
P Trieu-Cuot ◽  
P Courvalin
Keyword(s):  
Conjugative Transposition

scholarly journals Conjugative transposition of Tn916: the transposon int gene is required only in the donor.

1992 ◽  
Vol 174 (12) ◽  
pp. 4036-4041 ◽  
Author(s):  
F Bringel ◽  
G L Van Alstine ◽  
J R Scott
Keyword(s):  
Conjugative Transposition

scholarly journals The Frequency of Conjugative Transposition of Tn916 Is Not Determined by the Frequency of Excision

1999 ◽  
Vol 181 (17) ◽  
pp. 5414-5418 ◽  
Author(s):  
Diana Marra ◽  
Beth Pethel ◽  
Gordon G. Churchward ◽  
June R. Scott
Keyword(s):  
Transfer Functions ◽  
Recipient Cell ◽  
Inducible Promoter ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Conjugative Transposition ◽  
Origin Of Transfer

ABSTRACT Excision and formation of a covalently closed circular transposon molecule are required for conjugative transposition of Tn916 but are not the only factors that limit the frequency of conjugative transposition from one host to another. We found that in gram-positive bacteria, an increase in the frequency of excision and circularization of Tn916 caused by expression of integrase (Int) and excisionase (Xis) from a xylose-inducible promoter does not lead to an increase in the frequency of conjugative transposition. We also found that the concentration of Int and Xis in the recipient cell does not limit the frequency of conjugative transposition and that increased excision does not result in increased expression of transfer functions required to mobilize a plasmid containing the Tn916 origin of transfer. We conclude that in gram-positive hosts in which the Tn916 functions Int and Xis are overexpressed, the frequency of conjugative transposition is limited by the availability of transfer functions.

Conjugative Transposition

1995 ◽  
Vol 49 (1) ◽  
pp. 367-397 ◽  
Author(s):  
J R Scott ◽  
G G Churchward
Keyword(s):  
Conjugative Transposition

Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate

1994 ◽  
Vol 11 (6) ◽  
pp. 1099-1108 ◽  
Author(s):  
June R. Scott ◽  
Françoise Bringel ◽  
Diana Marra ◽  
Gaylene Alstine ◽  
Christine K. Rudy
Keyword(s):  
Single Strand ◽  
Conjugative Transfer ◽  
Conjugative Transposition

Conjugative transposition of Tn916 and Tn925 in Bacillus popilliae

1999 ◽  
Vol 45 (6) ◽  
pp. 530-535 ◽  
Author(s):  
Douglas W Dingman
Keyword(s):  
Bacillus Subtilis ◽  
Key Words ◽  
Enterococcus Faecalis ◽  
Selective Pressure ◽  
Conjugative Transposon ◽  
E Coli ◽  
Conjugative Transposons ◽  
Conjugative Transposition ◽  
Bacillus Popilliae

Interspecies transfer of the conjugative transposons Tn916 and Tn925 into B. popilliae Pj1 occurred using Enterococcus faecalis and Bacillus subtilis CU4049 as transposon donors. Tn916 was stably maintained in B. popilliae Pj1 following growth without selective pressure and was successfully introduced into the plasmid-containing B. popilliae strains NRRL B-2524, Ch1, and KLN4 using E. faecalis CG110. In B. popilliae, expression of the tetracycline resistant determinants on Tn916 and Tn925 provided resistance to 25 μg/mL and 50 μg/mL tetracycline, respectively. An erythromycin resistant determinant, present in Tn916ΔE, was also functional in B. popilliae Pj1 and provided resistance to 1 mg/mL erythromycin. Transfer of Tn916 into E. faecalis, B. subtilis, and between B. popilliae strains was accomplished using a transposon-containing strain of B. popilliae as donor. Efforts to transfer Tn916 between E. coli and B. popilliae were unsuccessful. Key words: Bacillus popilliae, milky disease, Tn916, conjugative transposon.

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