Conjugative Transposition

2001 ◽  
pp. 455-456
Author(s):  
G. Churchward
1991 ◽  
Vol 173 (14) ◽  
pp. 4347-4352 ◽  
Author(s):  
M J Storrs ◽  
C Poyart-Salmeron ◽  
P Trieu-Cuot ◽  
P Courvalin

1992 ◽  
Vol 174 (12) ◽  
pp. 4036-4041 ◽  
Author(s):  
F Bringel ◽  
G L Van Alstine ◽  
J R Scott

1999 ◽  
Vol 181 (17) ◽  
pp. 5414-5418 ◽  
Author(s):  
Diana Marra ◽  
Beth Pethel ◽  
Gordon G. Churchward ◽  
June R. Scott

ABSTRACT Excision and formation of a covalently closed circular transposon molecule are required for conjugative transposition of Tn916 but are not the only factors that limit the frequency of conjugative transposition from one host to another. We found that in gram-positive bacteria, an increase in the frequency of excision and circularization of Tn916 caused by expression of integrase (Int) and excisionase (Xis) from a xylose-inducible promoter does not lead to an increase in the frequency of conjugative transposition. We also found that the concentration of Int and Xis in the recipient cell does not limit the frequency of conjugative transposition and that increased excision does not result in increased expression of transfer functions required to mobilize a plasmid containing the Tn916 origin of transfer. We conclude that in gram-positive hosts in which the Tn916 functions Int and Xis are overexpressed, the frequency of conjugative transposition is limited by the availability of transfer functions.


1995 ◽  
Vol 49 (1) ◽  
pp. 367-397 ◽  
Author(s):  
J R Scott ◽  
G G Churchward

1994 ◽  
Vol 11 (6) ◽  
pp. 1099-1108 ◽  
Author(s):  
June R. Scott ◽  
Françoise Bringel ◽  
Diana Marra ◽  
Gaylene Alstine ◽  
Christine K. Rudy

1999 ◽  
Vol 45 (6) ◽  
pp. 530-535 ◽  
Author(s):  
Douglas W Dingman

Interspecies transfer of the conjugative transposons Tn916 and Tn925 into B. popilliae Pj1 occurred using Enterococcus faecalis and Bacillus subtilis CU4049 as transposon donors. Tn916 was stably maintained in B. popilliae Pj1 following growth without selective pressure and was successfully introduced into the plasmid-containing B. popilliae strains NRRL B-2524, Ch1, and KLN4 using E. faecalis CG110. In B. popilliae, expression of the tetracycline resistant determinants on Tn916 and Tn925 provided resistance to 25 μg/mL and 50 μg/mL tetracycline, respectively. An erythromycin resistant determinant, present in Tn916ΔE, was also functional in B. popilliae Pj1 and provided resistance to 1 mg/mL erythromycin. Transfer of Tn916 into E. faecalis, B. subtilis, and between B. popilliae strains was accomplished using a transposon-containing strain of B. popilliae as donor. Efforts to transfer Tn916 between E. coli and B. popilliae were unsuccessful. Key words: Bacillus popilliae, milky disease, Tn916, conjugative transposon.


Sign in / Sign up

Export Citation Format

Share Document