A Hybrid Approach to the 3D High Precision Position Measurement and Particle Tracking in Human Cell Nuclei

2002 ◽  
Vol 8 (6) ◽  
pp. 467-473
Author(s):  
A Cavallo
Keyword(s):  
High Precision ◽  
Human Cell ◽  
Particle Tracking ◽  
Hybrid Approach ◽  
Cell Nuclei ◽  
Position Measurement

scholarly journals Parallel, linear, and subnanometric 3D tracking of microparticles with Stereo Darkfield Interferometry

2021 ◽  
Vol 7 (6) ◽  
pp. eabe3902
Author(s):  
Martin Rieu ◽  
Thibault Vieille ◽  
Gaël Radou ◽  
Raphaël Jeanneret ◽  
Nadia Ruiz-Gutierrez ◽  
...  
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Single Molecule ◽  
Particle Tracking ◽  
Focal Plane ◽  
Tracking System ◽  
Three Dimensional ◽  
3D Tracking ◽  
Single Molecule Force Spectroscopy ◽  
A New Technique

While crucial for force spectroscopists and microbiologists, three-dimensional (3D) particle tracking suffers from either poor precision, complex calibration, or the need of expensive hardware, preventing its massive adoption. We introduce a new technique, based on a simple piece of cardboard inserted in the objective focal plane, that enables simple 3D tracking of dilute microparticles while offering subnanometer frame-to-frame precision in all directions. Its linearity alleviates calibration procedures, while the interferometric pattern enhances precision. We illustrate its utility in single-molecule force spectroscopy and single-algae motility analysis. As with any technique based on back focal plane engineering, it may be directly embedded in a commercial objective, providing a means to convert any preexisting optical setup in a 3D tracking system. Thanks to its precision, its simplicity, and its versatility, we envision that the technique has the potential to enhance the spreading of high-precision and high-throughput 3D tracking.

scholarly journals Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy

2005 ◽  
Vol 88 (6) ◽  
pp. 4312-4318 ◽  
Author(s):  
Georg Hildenbrand ◽  
Alexander Rapp ◽  
Udo Spöri ◽  
Christian Wagner ◽  
Christoph Cremer ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Human Cell ◽  
Specific Gene ◽  
Cell Nuclei

scholarly journals Evaluation of Quantitative Approaches for High Precision 3D Single Particle Tracking in Thick Cellular Specimens using Multifocal Plane Microscopy

2012 ◽  
Vol 102 (3) ◽  
pp. 194a
Author(s):  
Ramraj Velmurugan ◽  
Sripad Ram ◽  
Anish V. Abraham ◽  
Jerry Chao ◽  
Andrea Grosso ◽  
...  
Keyword(s):  
High Precision ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Quantitative Approaches

scholarly journals An avidin-based assay for histone debiotinylase activity in human cell nuclei

2007 ◽  
Vol 18 (7) ◽  
pp. 475-481 ◽  
Author(s):  
Yap Ching Chew ◽  
Gautam Sarath ◽  
Janos Zempleni
Keyword(s):  
Human Cell ◽  
Cell Nuclei

A Dynamic Position Measurement Method for Destructive Process of Structures Using Image Processing

2004 ◽  
Author(s):  
Hiroshi Nishizawa ◽  
Satoshi Fujita ◽  
Osamu Furuya
Keyword(s):  
Image Processing ◽  
High Precision ◽  
High Speed ◽  
Spherical Surface ◽  
Three Dimensional ◽  
Measuring System ◽  
Position Measurement ◽  
Surface Emission ◽  
Optical Motion ◽  
Optical Motion Capture

In order to clarify the destruction mechanism of large structures in large seismic movements, a non-contacting displacement measurement system with a three-dimensional dynamic position with high precision is required. We have developed a three-dimensional measuring system with image processing using optical motion capture technology. This system consists of light emitting markers installed on the object structure and plural high speed cameras which obtain images of markers’ movement simultaneously, to measure the dynamic position of the three dimensional spatial coordinates of the markers. In order to measure the dynamic position with high precision, we have ever developed sub-pixel processing method which is able to measure very small displacements of the markers by analyzing the luminance distribution. Moreover, we have developed a new marker of spherical surface emission type which formed the luminance profile to improve furthermore the accuracy in rotational movement. Shaking tests were carried out with this measuring system and the results indicated that this system using new markers had sufficient accuracy within errors of a few millimeters in the structure of a 4 meter cube. Consequently, we have acquired the potential to apply to the measurement to the 3-D Full Scale Earthquake Testing Facility (E-Defense).

scholarly journals High-precision Optical Position Measurement in Indoor Environments

2015 ◽  
Vol 10 (3) ◽  
pp. 24-27 ◽  
Author(s):  
Ralf Vandenhouten ◽  
Ole Wendlandt
Keyword(s):  
High Precision ◽  
Indoor Environments ◽  
Position Measurement ◽  
Optical Position
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