N-Acetylcysteine Effect on the Luminol-dependent Chemiluminescence Pattern of Reactive Oxygen Species Generation by Human Polymorphonuclear Leukocytes

2002 ◽  
Vol 15 (4) ◽  
pp. 385-392 ◽  
Author(s):  
Robert Stolarek ◽  
Piotr Białasiewicz ◽  
Dariusz Nowak
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Polymorphonuclear Leukocytes

Bacteriophage preparation inhibition of reactive oxygen species generation by endotoxin-stimulated polymorphonuclear leukocytes

2008 ◽  
Vol 131 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Ryszard Miedzybrodzki ◽  
Kinga Switala-Jelen ◽  
Wojciech Fortuna ◽  
Beata Weber-Dabrowska ◽  
Anna Przerwa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species

Production of reactive oxygen species by man-made vitreous fibres in human polymorphonuclear leukocytes

1999 ◽  
Vol 18 (6) ◽  
pp. 354-362 ◽  
Author(s):  
Marjo Ruotsalainen ◽  
Maija-Riitta Hirvonen ◽  
Kirsi Luoto ◽  
Kai M. Savolainen
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Cell Activation ◽  
Fibre Length ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Trypan Blue Exclusion ◽  
Human Polymorphonuclear Leukocytes ◽  
Dose Dependent ◽  
Positive Controls

Human polymorphonuclear leukocytes (PMNL) or erythrocytes, isolated from human blood, were exposed to graded doses of asbestos (chrysotile), quartz, or man-made vitreous fibres (MMVF), i.e. refractory ceramic fibres (RCF), glasswool, or rockwool fibres. None of the MMVF affected either the viability of PMNL, as measured by trypan blue exclusion test, or induced haemolysis, whereas the positive controls, quartz and chrysotile, dose-dependently induced haemolysis in PMNL. MMVF did not increase the release of lactate dehydrogenase (LDH) from the PMNL, whereas the positive controls, chrysotile and quartz, induced a marked and dose-dependent release of LDH. When PMNL were exposed to MMVF, some of the fibre types slightly increased the levels of free intracellular calcium ([Ca2+]i)withinthecellsina manner similar to that induced by chrysotile or quartz. All MMVF induced a dose-dependent production of reactive oxygen species (ROS) in PMNL, with RCF-induced production of ROS being the most marked. Production of ROS by MMVF seemed to depend on the availability of extracellular calcium because it could be attenuated with aCa2+ channel blocker, verapamil, or a Ca2+ chelating agent, EGTA. Production of ROS may be a common pathway through which PMNL respond to MMVF-induced cell activation, but alterations of levels of free intracellular Ca2+ do not seem to be an absolute prerequisite for this effect. Fibre length seemed not to be an important factor in affecting the ability of MMVF to induce ROS production in PMNL. However, the balance between different elements in the fibre seemed importantly to affect the biological activity of a fibre.

The Essential Oil of Bergamot Stimulates Reactive Oxygen Species Production in Human Polymorphonuclear Leukocytes

2014 ◽  
Vol 28 (8) ◽  
pp. 1232-1239 ◽  
Author(s):  
Marco Cosentino ◽  
Alessandra Luini ◽  
Raffaella Bombelli ◽  
Maria T. Corasaniti ◽  
Giacinto Bagetta ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Essential Oil ◽  
Polymorphonuclear Leukocytes ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Production ◽  
Human Polymorphonuclear Leukocytes
Sign in / Sign up

Export Citation Format

Share Document