Cost-Effective and Uniform 13C- and 15N-Labeling of the 24-kDa N-Terminal Domain of the Escherichia coli Gyrase B by Overexpression in the Photoautotrophic Cyanobacterium Anabaena sp. PCC 7120

2001 ◽  
Vol 23 (1) ◽  
pp. 207-217 ◽  
Author(s):  
Dominique Desplancq ◽  
Bruno Kieffer ◽  
Karsten Schmidt ◽  
Clemens Posten ◽  
Anne Forster ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cost Effective ◽  
Terminal Domain ◽  
Cyanobacterium Anabaena ◽  
15N Labeling ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120

2016 ◽  
Vol 171 (3) ◽  
pp. 1852-1866 ◽  
Author(s):  
Rocío López-Igual ◽  
Adjélé Wilson ◽  
Ryan L. Leverenz ◽  
Matthew R. Melnicki ◽  
Céline Bourcier de Carbon ◽  
...  
Keyword(s):  
Terminal Domain ◽  
Cyanobacterium Anabaena ◽  
Orange Carotenoid Protein ◽  
Anabaena Sp ◽  
Pcc 7120

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Spatio-Temporal Gene Induction Systems in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

2017 ◽  
Vol 59 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Akiyoshi Higo ◽  
Atsuko Isu ◽  
Yuki Fukaya ◽  
Toru Hisabori
Keyword(s):  
Gene Induction ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Spatio Temporal ◽  
Pcc 7120

scholarly journals Manipulation of Pattern of Cell Differentiation in a hetR Mutant of Anabaena sp. PCC 7120 by Overexpressing hetZ Alone or with hetP

Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 60 ◽  
Author(s):  
He Zhang ◽  
Xudong Xu
Keyword(s):  
Cell Differentiation ◽  
Peptide Inhibitor ◽  
Filamentous Cyanobacterium ◽  
Heterocyst Differentiation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

In the filamentous cyanobacterium, Anabaena sp. PCC 7120, single heterocysts differentiate at semi-regular intervals in response to nitrogen stepdown. HetR is a principal regulator of heterocyst differentiation, and hetP and hetZ are two genes that are regulated directly by HetR. In a hetR mutant generated from the IHB (Institute of Hydrobiology) substrain of PCC 7120, heterocyst formation can be restored by moderate expression of hetZ and hetP. The resulting heterocysts are located at terminal positions. We used a tandem promoter, PrbcLPpetE, to express hetZ and hetP strongly in the hetR mutant. Co-expression of hetZ and hetP enabled the hetR mutant to form multiple contiguous heterocysts at both terminal and intercalary positions. Expression of hetZ, alone resulted in terminally located heterocysts, whereas expression of hetP, alone produced enlarged cells in strings. In the absence of HetR, formation of heterocysts was insensitive to the peptide inhibitor, RGSGR.

scholarly journals Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Vol 9 (1) ◽  
pp. 63-75 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn N. Wells ◽  
Arun J. Singh ◽  
Jessie Eiting ◽  
Philip J. Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Heterologous Expression ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120
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